Contributions
Abstract: P04
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
Apheresis and storage of platelet concentrates (PCs) affected by the platelets activation and total functional capacity of these cells. We assume that after transfusion the prevalence of platelets with changed activity lead to worse quality of blood clot in vivo. The aim was the in vitro study of platelet-dependent clot properties as a function of storage time.
Methods
Fifty single-donor apheresis PCs were divided in two groups: group 1 - platelets were remained in autologous plasma (PCs-P; n=26); group 2 – platelets were resuspended in platelet additive solution (PAS) which substituted up to 70 vol% of autoplasma (PCs-PAS; n=24). Storage conditions were equal. PCs samples were analyzed by modified thromboelastography, and by aggregometry, and for platelets count, pH, lactate, glucose, and other platelets parameters. The testing were carried out in the day of proceeding, after 24 hours, and at 3rd and 5th days of storage. Dates were present as median (95% CI). Statistical differences were calculated using Mann-Whitney test (p<0,05), besides regression analysis was performed.
Results
Between PCs-P and PCs-PAS no significantly differences had for platelets count. From the day of producing to the 5th days of storage glucose decreased in PCs-P from 18,3 mmol/L to 9,4 mmol/L (-48.6%), and in PSc-PAS from 5,2 mmol/L to 1,3 mmol/L (-52%), and lactate concentration had the increase in PCs-P from 2,7 mmol/L to 16,4 mmol/L (6-fold up), and in PCs-PAS from 1,4 mmol/L to 9,6 mmol/L (6,9-fold up). However pH was almost unchanged that indicated buffer conditions were good in both types of PCs. During the storage platelets aggregability and adhesion had worsened independently PCs type. Platelet aggregation decreased in PCs-P from the day of producing to the 5th days of storage ADP-induced by 44%, collagen-induced by 29,5%, ristomycin-induced by 40,4%. In PCs-PAS platelet aggregation decrease in response to ADP, collagen, ristomycin was 44%, 30%, 26%, respectively. We found that clot demonstrated gradual reduction of elasticity and deformability in both PCs groups (in PCs-P: Angle -30%, МА -9%, G -24%; in PCs-PAS: Angle -19%, МА -13%, G -29%). According to regression analysis in PCs-P platelets lost their meaning for clot properties from the third storage day, in PCs-PAS activated platelets had no impact to clot properties during full storage time.
Conclusion
Irrespective of the proceeding method platelets viability was saved during the first five days of storage. Platelets apheresis and storage are accompanied by aggregation-and- adhesion activity depression. It could be speculated that storage impairs platelets granules secretion and thromboxane A2 synthesis, and cell-cell interaction. We found total decline of clot quality including low elasticity and impaired deformability during of storage time. We assume that clot properties are forming at the day of proceeding. Therefore we suppose that effect PCs transfusion is related to successful of platelets activity recovery in vivo.
Abstract: P04
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
Apheresis and storage of platelet concentrates (PCs) affected by the platelets activation and total functional capacity of these cells. We assume that after transfusion the prevalence of platelets with changed activity lead to worse quality of blood clot in vivo. The aim was the in vitro study of platelet-dependent clot properties as a function of storage time.
Methods
Fifty single-donor apheresis PCs were divided in two groups: group 1 - platelets were remained in autologous plasma (PCs-P; n=26); group 2 – platelets were resuspended in platelet additive solution (PAS) which substituted up to 70 vol% of autoplasma (PCs-PAS; n=24). Storage conditions were equal. PCs samples were analyzed by modified thromboelastography, and by aggregometry, and for platelets count, pH, lactate, glucose, and other platelets parameters. The testing were carried out in the day of proceeding, after 24 hours, and at 3rd and 5th days of storage. Dates were present as median (95% CI). Statistical differences were calculated using Mann-Whitney test (p<0,05), besides regression analysis was performed.
Results
Between PCs-P and PCs-PAS no significantly differences had for platelets count. From the day of producing to the 5th days of storage glucose decreased in PCs-P from 18,3 mmol/L to 9,4 mmol/L (-48.6%), and in PSc-PAS from 5,2 mmol/L to 1,3 mmol/L (-52%), and lactate concentration had the increase in PCs-P from 2,7 mmol/L to 16,4 mmol/L (6-fold up), and in PCs-PAS from 1,4 mmol/L to 9,6 mmol/L (6,9-fold up). However pH was almost unchanged that indicated buffer conditions were good in both types of PCs. During the storage platelets aggregability and adhesion had worsened independently PCs type. Platelet aggregation decreased in PCs-P from the day of producing to the 5th days of storage ADP-induced by 44%, collagen-induced by 29,5%, ristomycin-induced by 40,4%. In PCs-PAS platelet aggregation decrease in response to ADP, collagen, ristomycin was 44%, 30%, 26%, respectively. We found that clot demonstrated gradual reduction of elasticity and deformability in both PCs groups (in PCs-P: Angle -30%, МА -9%, G -24%; in PCs-PAS: Angle -19%, МА -13%, G -29%). According to regression analysis in PCs-P platelets lost their meaning for clot properties from the third storage day, in PCs-PAS activated platelets had no impact to clot properties during full storage time.
Conclusion
Irrespective of the proceeding method platelets viability was saved during the first five days of storage. Platelets apheresis and storage are accompanied by aggregation-and- adhesion activity depression. It could be speculated that storage impairs platelets granules secretion and thromboxane A2 synthesis, and cell-cell interaction. We found total decline of clot quality including low elasticity and impaired deformability during of storage time. We assume that clot properties are forming at the day of proceeding. Therefore we suppose that effect PCs transfusion is related to successful of platelets activity recovery in vivo.