THE EFFECT OF MESENCHYMAL STEM CELLS DERIVED FROM DIFFERENT SOURCES IN COMBINATION WITH HAPLOIDENTICAL HSCT IN THE TREATMENT OF BONE MARROW TYPE RADIATION SICKNESS
(Abstract release date: 05/19/16)
EHA Library. ZHANG C. 06/09/16; 135372; LB2261
Prof. Cheng ZHANG
Contributions
Contributions
Abstract
Abstract: LB2261
Type: Eposter Presentation
Background
Mesenchymal stem cells (MSCs) in the microenvironment can come from different origins and exhibit a diversity of properties. However, source-dependent and donor-dependent differences in MSCs properties, including their clinical application are still largely disputed.
Aims
To investigate the effect of the transplantation of mesenchymal stem cells (MSC) derived from different sources in combination with hematopoietic stem cells (HSC) in the treatment of bone marrow type radiation sickness.
Methods
100 mice (CB6F1(H-2b×d)) were hybrids of C57BL/6 (H-2b) and BABL/C (H-2d), 8 weeks old, weight 18g-22g,and were divided into 5 groups after 8.5Gy total body irradiation (TBI) of 60Coγray, according to a completely randomized design, irradiation group (n=20), HSC transplantation group (n=20), human umbilical cord blood MSCs (HucbMSCs) and HSC cotransplantation group (n=20), bone marrow MSCs (BMMSCs) and HSC cotransplantation group (n=20), dermal MSCs (DMSCs) and HSC cotransplantation group (n=20). The clinical manifestation of mice were observed after transplantation. The survival rate, pathological changes of BM, and other indicators were tested.
Results
Hair back, decreased activity, diarrhea and weight loss appeared at three days after irradiation. At the 11th day, all mice in irradiation group died while the amount of eating and drinking increased and body weight began to recover in the transplantation group. The clinical manifestation score of GVHD in cotransplantation group was lower than that in single transplantation. At 28th day after irradiation, the survival rate of mice in HucbMSCs group was 95%,which were 85%, 80% and 70% in BMMSCs group, DMSC group and transplantation group respectively. The hematopoietic reconstitution taperoffed in cotransplantation group, transplantation group, HucbMSCs group, BMMSCs group, and DMSCs group. The lowest value of hemogram were appear at 3-7d of WBC, 7-10d of PLT, and 10-14d of RBC after transplantation. The pathological change and the colony formation ability of BM were consistent with the change in hemogram.
Conclusion
MSCs could promote hematopoietic reconstitution and reduce GVHD effect in bone marrow type radiation sickness. HucbMSCs has better application prospect than others in bone marrow type radiation sickness.
Session topic: E-poster
Keyword(s): Hematopoietic cell transplantation, Mesenchymal stem cell, Myelodysplasia
Type: Eposter Presentation
Background
Mesenchymal stem cells (MSCs) in the microenvironment can come from different origins and exhibit a diversity of properties. However, source-dependent and donor-dependent differences in MSCs properties, including their clinical application are still largely disputed.
Aims
To investigate the effect of the transplantation of mesenchymal stem cells (MSC) derived from different sources in combination with hematopoietic stem cells (HSC) in the treatment of bone marrow type radiation sickness.
Methods
100 mice (CB6F1(H-2b×d)) were hybrids of C57BL/6 (H-2b) and BABL/C (H-2d), 8 weeks old, weight 18g-22g,and were divided into 5 groups after 8.5Gy total body irradiation (TBI) of 60Coγray, according to a completely randomized design, irradiation group (n=20), HSC transplantation group (n=20), human umbilical cord blood MSCs (HucbMSCs) and HSC cotransplantation group (n=20), bone marrow MSCs (BMMSCs) and HSC cotransplantation group (n=20), dermal MSCs (DMSCs) and HSC cotransplantation group (n=20). The clinical manifestation of mice were observed after transplantation. The survival rate, pathological changes of BM, and other indicators were tested.
Results
Hair back, decreased activity, diarrhea and weight loss appeared at three days after irradiation. At the 11th day, all mice in irradiation group died while the amount of eating and drinking increased and body weight began to recover in the transplantation group. The clinical manifestation score of GVHD in cotransplantation group was lower than that in single transplantation. At 28th day after irradiation, the survival rate of mice in HucbMSCs group was 95%,which were 85%, 80% and 70% in BMMSCs group, DMSC group and transplantation group respectively. The hematopoietic reconstitution taperoffed in cotransplantation group, transplantation group, HucbMSCs group, BMMSCs group, and DMSCs group. The lowest value of hemogram were appear at 3-7d of WBC, 7-10d of PLT, and 10-14d of RBC after transplantation. The pathological change and the colony formation ability of BM were consistent with the change in hemogram.
Conclusion
MSCs could promote hematopoietic reconstitution and reduce GVHD effect in bone marrow type radiation sickness. HucbMSCs has better application prospect than others in bone marrow type radiation sickness.
Session topic: E-poster
Keyword(s): Hematopoietic cell transplantation, Mesenchymal stem cell, Myelodysplasia
Abstract: LB2261
Type: Eposter Presentation
Background
Mesenchymal stem cells (MSCs) in the microenvironment can come from different origins and exhibit a diversity of properties. However, source-dependent and donor-dependent differences in MSCs properties, including their clinical application are still largely disputed.
Aims
To investigate the effect of the transplantation of mesenchymal stem cells (MSC) derived from different sources in combination with hematopoietic stem cells (HSC) in the treatment of bone marrow type radiation sickness.
Methods
100 mice (CB6F1(H-2b×d)) were hybrids of C57BL/6 (H-2b) and BABL/C (H-2d), 8 weeks old, weight 18g-22g,and were divided into 5 groups after 8.5Gy total body irradiation (TBI) of 60Coγray, according to a completely randomized design, irradiation group (n=20), HSC transplantation group (n=20), human umbilical cord blood MSCs (HucbMSCs) and HSC cotransplantation group (n=20), bone marrow MSCs (BMMSCs) and HSC cotransplantation group (n=20), dermal MSCs (DMSCs) and HSC cotransplantation group (n=20). The clinical manifestation of mice were observed after transplantation. The survival rate, pathological changes of BM, and other indicators were tested.
Results
Hair back, decreased activity, diarrhea and weight loss appeared at three days after irradiation. At the 11th day, all mice in irradiation group died while the amount of eating and drinking increased and body weight began to recover in the transplantation group. The clinical manifestation score of GVHD in cotransplantation group was lower than that in single transplantation. At 28th day after irradiation, the survival rate of mice in HucbMSCs group was 95%,which were 85%, 80% and 70% in BMMSCs group, DMSC group and transplantation group respectively. The hematopoietic reconstitution taperoffed in cotransplantation group, transplantation group, HucbMSCs group, BMMSCs group, and DMSCs group. The lowest value of hemogram were appear at 3-7d of WBC, 7-10d of PLT, and 10-14d of RBC after transplantation. The pathological change and the colony formation ability of BM were consistent with the change in hemogram.
Conclusion
MSCs could promote hematopoietic reconstitution and reduce GVHD effect in bone marrow type radiation sickness. HucbMSCs has better application prospect than others in bone marrow type radiation sickness.
Session topic: E-poster
Keyword(s): Hematopoietic cell transplantation, Mesenchymal stem cell, Myelodysplasia
Type: Eposter Presentation
Background
Mesenchymal stem cells (MSCs) in the microenvironment can come from different origins and exhibit a diversity of properties. However, source-dependent and donor-dependent differences in MSCs properties, including their clinical application are still largely disputed.
Aims
To investigate the effect of the transplantation of mesenchymal stem cells (MSC) derived from different sources in combination with hematopoietic stem cells (HSC) in the treatment of bone marrow type radiation sickness.
Methods
100 mice (CB6F1(H-2b×d)) were hybrids of C57BL/6 (H-2b) and BABL/C (H-2d), 8 weeks old, weight 18g-22g,and were divided into 5 groups after 8.5Gy total body irradiation (TBI) of 60Coγray, according to a completely randomized design, irradiation group (n=20), HSC transplantation group (n=20), human umbilical cord blood MSCs (HucbMSCs) and HSC cotransplantation group (n=20), bone marrow MSCs (BMMSCs) and HSC cotransplantation group (n=20), dermal MSCs (DMSCs) and HSC cotransplantation group (n=20). The clinical manifestation of mice were observed after transplantation. The survival rate, pathological changes of BM, and other indicators were tested.
Results
Hair back, decreased activity, diarrhea and weight loss appeared at three days after irradiation. At the 11th day, all mice in irradiation group died while the amount of eating and drinking increased and body weight began to recover in the transplantation group. The clinical manifestation score of GVHD in cotransplantation group was lower than that in single transplantation. At 28th day after irradiation, the survival rate of mice in HucbMSCs group was 95%,which were 85%, 80% and 70% in BMMSCs group, DMSC group and transplantation group respectively. The hematopoietic reconstitution taperoffed in cotransplantation group, transplantation group, HucbMSCs group, BMMSCs group, and DMSCs group. The lowest value of hemogram were appear at 3-7d of WBC, 7-10d of PLT, and 10-14d of RBC after transplantation. The pathological change and the colony formation ability of BM were consistent with the change in hemogram.
Conclusion
MSCs could promote hematopoietic reconstitution and reduce GVHD effect in bone marrow type radiation sickness. HucbMSCs has better application prospect than others in bone marrow type radiation sickness.
Session topic: E-poster
Keyword(s): Hematopoietic cell transplantation, Mesenchymal stem cell, Myelodysplasia
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