HUMAN UMBILICAL CORD BLOOD STROMAL CELLS PROMOTE HEMATOPOIETIC RECONSTITUTION AND REDUCE GVHD BY AFFECTING THE TH CELL SUBSETS IN HAPLOIDENTICAL-HSCT
(Abstract release date: 05/19/16)
EHA Library. ZHANG C. 06/09/16; 135368; LB2257
Prof. Cheng ZHANG
Contributions
Contributions
Abstract
Abstract: LB2257
Type: Eposter Presentation
Background
Microenviroment palys very important role in the regulation of graft-versus-host desease (GVHD).
Aims
To investigate the effect of human cord blood–derived stromal cells (hUCBDSCs) combined with haploidentical-hematopoietic stem cell transplantation (haplo-HSCT) on GVHD and hematopoietic reconstitution of severe bone marrow radiation disease.
Methods
The hybrids F1 generation mice were taken 8.0Gy of 60Coγ ray total body irradiation (TBI). Haploidentical-HSCT were taken after TBI. Grouping: mBMSC (mouse bone marrow-derived stromal cells) + HSC(Hematopoietic stem cells) group, hUCBDSCs + HSC group, HSC group and the control group (saline group). Peripheral blood were taken at 0,3,7,10,14,21,28d days respectively after transplantation to count WBC, RBC, Hb, PLT. Bone marrow, liver, spleen, intestine and skin were collected and paraffin-embedded after transplantation at each point to observe pathologic changes . The changes of body weight, postural, activity, hair, toilet and survival were observed after transplantation at each point. Cell colony culture were taken at 7,14,21d after transplantation. IL-2, IL-4, IL-10, IL-12, IL-17, IL-21, IL-22, IL- 23, IL-33, TGF-β, IFN-α and IFN-γ in serum were detected by ELISA at 0,7,14,21d after transplantation.
Results
All mice in control group died at 9d to 11d after TBI. The hemogram began to recover at 7d in hUCBDSCs + HSC group and mBMSC + HSC group after transplantation which was earlier than that in HSC group （10d）. Cell colony of CFU-E, CFU-GM, CFU-GEMM and CFU-G in hUCBDSCs + HSC group was more than mBMSC + HSC group (p <0.05). And mBMSC + HSC group was more than HSC group (p <0.05). The death rate of hUCBDSCs + HSC group and mBMSC + HSC group were (20.00±5.00)% and (21.67±12.58)% which were lower than (38.33±7.64) % in HSC group (p <0.05). The GVHD of hUCBDSCs + HSC group was not obviously which was better than HSC group. There were no significant differences of IL-12, IL-17 and IL-23 in each group. IL-2, IFN-γ expression were increased after transplantation, which is higher in HSC group than hUCBDSCs group(p <0.05); IL-10 expression in the peripheral blood were decreased in each group after transplantation. The recovery of IL-10 in hUCBDSCs + HSC group was faster than HSC group (p <0.05). TGF-β expression were increased faster in hUCBDSCs + HSC group than the other two groups(p <0.05) which was the same as TNF -α.
Conclusion
HUCBDSCs promote hematopoietic reconstitution and reduce the incidence of GVHD in severe bone marrow radiation disease in vivo. The main mechanism of this may be associated with the decrease of Th1 cells and increase of Th2 cells expression.
Session topic: E-poster
Keyword(s): Microenvironment, Stromal cell
Type: Eposter Presentation
Background
Microenviroment palys very important role in the regulation of graft-versus-host desease (GVHD).
Aims
To investigate the effect of human cord blood–derived stromal cells (hUCBDSCs) combined with haploidentical-hematopoietic stem cell transplantation (haplo-HSCT) on GVHD and hematopoietic reconstitution of severe bone marrow radiation disease.
Methods
The hybrids F1 generation mice were taken 8.0Gy of 60Coγ ray total body irradiation (TBI). Haploidentical-HSCT were taken after TBI. Grouping: mBMSC (mouse bone marrow-derived stromal cells) + HSC(Hematopoietic stem cells) group, hUCBDSCs + HSC group, HSC group and the control group (saline group). Peripheral blood were taken at 0,3,7,10,14,21,28d days respectively after transplantation to count WBC, RBC, Hb, PLT. Bone marrow, liver, spleen, intestine and skin were collected and paraffin-embedded after transplantation at each point to observe pathologic changes . The changes of body weight, postural, activity, hair, toilet and survival were observed after transplantation at each point. Cell colony culture were taken at 7,14,21d after transplantation. IL-2, IL-4, IL-10, IL-12, IL-17, IL-21, IL-22, IL- 23, IL-33, TGF-β, IFN-α and IFN-γ in serum were detected by ELISA at 0,7,14,21d after transplantation.
Results
All mice in control group died at 9d to 11d after TBI. The hemogram began to recover at 7d in hUCBDSCs + HSC group and mBMSC + HSC group after transplantation which was earlier than that in HSC group （10d）. Cell colony of CFU-E, CFU-GM, CFU-GEMM and CFU-G in hUCBDSCs + HSC group was more than mBMSC + HSC group (p <0.05). And mBMSC + HSC group was more than HSC group (p <0.05). The death rate of hUCBDSCs + HSC group and mBMSC + HSC group were (20.00±5.00)% and (21.67±12.58)% which were lower than (38.33±7.64) % in HSC group (p <0.05). The GVHD of hUCBDSCs + HSC group was not obviously which was better than HSC group. There were no significant differences of IL-12, IL-17 and IL-23 in each group. IL-2, IFN-γ expression were increased after transplantation, which is higher in HSC group than hUCBDSCs group(p <0.05); IL-10 expression in the peripheral blood were decreased in each group after transplantation. The recovery of IL-10 in hUCBDSCs + HSC group was faster than HSC group (p <0.05). TGF-β expression were increased faster in hUCBDSCs + HSC group than the other two groups(p <0.05) which was the same as TNF -α.
Conclusion
HUCBDSCs promote hematopoietic reconstitution and reduce the incidence of GVHD in severe bone marrow radiation disease in vivo. The main mechanism of this may be associated with the decrease of Th1 cells and increase of Th2 cells expression.
Session topic: E-poster
Keyword(s): Microenvironment, Stromal cell
Abstract: LB2257
Type: Eposter Presentation
Background
Microenviroment palys very important role in the regulation of graft-versus-host desease (GVHD).
Aims
To investigate the effect of human cord blood–derived stromal cells (hUCBDSCs) combined with haploidentical-hematopoietic stem cell transplantation (haplo-HSCT) on GVHD and hematopoietic reconstitution of severe bone marrow radiation disease.
Methods
The hybrids F1 generation mice were taken 8.0Gy of 60Coγ ray total body irradiation (TBI). Haploidentical-HSCT were taken after TBI. Grouping: mBMSC (mouse bone marrow-derived stromal cells) + HSC(Hematopoietic stem cells) group, hUCBDSCs + HSC group, HSC group and the control group (saline group). Peripheral blood were taken at 0,3,7,10,14,21,28d days respectively after transplantation to count WBC, RBC, Hb, PLT. Bone marrow, liver, spleen, intestine and skin were collected and paraffin-embedded after transplantation at each point to observe pathologic changes . The changes of body weight, postural, activity, hair, toilet and survival were observed after transplantation at each point. Cell colony culture were taken at 7,14,21d after transplantation. IL-2, IL-4, IL-10, IL-12, IL-17, IL-21, IL-22, IL- 23, IL-33, TGF-β, IFN-α and IFN-γ in serum were detected by ELISA at 0,7,14,21d after transplantation.
Results
All mice in control group died at 9d to 11d after TBI. The hemogram began to recover at 7d in hUCBDSCs + HSC group and mBMSC + HSC group after transplantation which was earlier than that in HSC group （10d）. Cell colony of CFU-E, CFU-GM, CFU-GEMM and CFU-G in hUCBDSCs + HSC group was more than mBMSC + HSC group (p <0.05). And mBMSC + HSC group was more than HSC group (p <0.05). The death rate of hUCBDSCs + HSC group and mBMSC + HSC group were (20.00±5.00)% and (21.67±12.58)% which were lower than (38.33±7.64) % in HSC group (p <0.05). The GVHD of hUCBDSCs + HSC group was not obviously which was better than HSC group. There were no significant differences of IL-12, IL-17 and IL-23 in each group. IL-2, IFN-γ expression were increased after transplantation, which is higher in HSC group than hUCBDSCs group(p <0.05); IL-10 expression in the peripheral blood were decreased in each group after transplantation. The recovery of IL-10 in hUCBDSCs + HSC group was faster than HSC group (p <0.05). TGF-β expression were increased faster in hUCBDSCs + HSC group than the other two groups(p <0.05) which was the same as TNF -α.
Conclusion
HUCBDSCs promote hematopoietic reconstitution and reduce the incidence of GVHD in severe bone marrow radiation disease in vivo. The main mechanism of this may be associated with the decrease of Th1 cells and increase of Th2 cells expression.
Session topic: E-poster
Keyword(s): Microenvironment, Stromal cell
Type: Eposter Presentation
Background
Microenviroment palys very important role in the regulation of graft-versus-host desease (GVHD).
Aims
To investigate the effect of human cord blood–derived stromal cells (hUCBDSCs) combined with haploidentical-hematopoietic stem cell transplantation (haplo-HSCT) on GVHD and hematopoietic reconstitution of severe bone marrow radiation disease.
Methods
The hybrids F1 generation mice were taken 8.0Gy of 60Coγ ray total body irradiation (TBI). Haploidentical-HSCT were taken after TBI. Grouping: mBMSC (mouse bone marrow-derived stromal cells) + HSC(Hematopoietic stem cells) group, hUCBDSCs + HSC group, HSC group and the control group (saline group). Peripheral blood were taken at 0,3,7,10,14,21,28d days respectively after transplantation to count WBC, RBC, Hb, PLT. Bone marrow, liver, spleen, intestine and skin were collected and paraffin-embedded after transplantation at each point to observe pathologic changes . The changes of body weight, postural, activity, hair, toilet and survival were observed after transplantation at each point. Cell colony culture were taken at 7,14,21d after transplantation. IL-2, IL-4, IL-10, IL-12, IL-17, IL-21, IL-22, IL- 23, IL-33, TGF-β, IFN-α and IFN-γ in serum were detected by ELISA at 0,7,14,21d after transplantation.
Results
All mice in control group died at 9d to 11d after TBI. The hemogram began to recover at 7d in hUCBDSCs + HSC group and mBMSC + HSC group after transplantation which was earlier than that in HSC group （10d）. Cell colony of CFU-E, CFU-GM, CFU-GEMM and CFU-G in hUCBDSCs + HSC group was more than mBMSC + HSC group (p <0.05). And mBMSC + HSC group was more than HSC group (p <0.05). The death rate of hUCBDSCs + HSC group and mBMSC + HSC group were (20.00±5.00)% and (21.67±12.58)% which were lower than (38.33±7.64) % in HSC group (p <0.05). The GVHD of hUCBDSCs + HSC group was not obviously which was better than HSC group. There were no significant differences of IL-12, IL-17 and IL-23 in each group. IL-2, IFN-γ expression were increased after transplantation, which is higher in HSC group than hUCBDSCs group(p <0.05); IL-10 expression in the peripheral blood were decreased in each group after transplantation. The recovery of IL-10 in hUCBDSCs + HSC group was faster than HSC group (p <0.05). TGF-β expression were increased faster in hUCBDSCs + HSC group than the other two groups(p <0.05) which was the same as TNF -α.
Conclusion
HUCBDSCs promote hematopoietic reconstitution and reduce the incidence of GVHD in severe bone marrow radiation disease in vivo. The main mechanism of this may be associated with the decrease of Th1 cells and increase of Th2 cells expression.
Session topic: E-poster
Keyword(s): Microenvironment, Stromal cell
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