SHOULD BLOOD DONORS WITH HARRIS PLATELET SYNDROME BE EXCLUDED FROM PLATELET DONATION?
(Abstract release date: 05/19/16)
EHA Library. Das A. 06/09/16; 135132; PB2232
Dr. Avash Das
Contributions
Contributions
Abstract
Abstract: PB2232
Type: Publication Only
Background
Harris platelet syndrome (HPS) is an autosomal dominant macrothrombocytopenia with mild to moderately severe thrombocytopenia and normal platelet function. HPS has been reported from Indian subcontinent blood donors, especially from the northeastern part of India. Approximately a third of blood donors from state of West Bengal have been found to have HPS.The Indian NACO 2007 guideline recommends one bag of PRC from 350 ml of whole blood should have a platelet count above 3.5 X10 9/l.
Aims
We designed a study to assess whether PRC (platelet rich concentrate) obtained from those with HPS contained adequate platelets as recommended by the Indian guidelines.
Methods
HPS is defined as healthy blood donors having a mean platelet volume (MPV) greater than 12 fL (normal <10 fL) with or without concomitant thrombocytopenia in the absence of any significant past history of bleeding disorder in either themselves or their first degree relatives.In our study, we collected 350 ml of blood from each donor and via Platelet Rich Plasma Separation Method (PRPSM) we separated out PRC and PRBC in separate bags.A CBC was performed at the time of donation and from PRC bag blood collected in EDTA tubes, and analyzed using SYSMEX XP-100.
Results
A total of 28 donors were included. The median age of the donors was 33 years(21-62) and 21 (75%) were male.Pre donation CBC showed a median platelet count of 1.93 ± 0.54 X109/ L (0.99-2.98 X109) and platelet count from PRC showed a median platelet count 1.16 ± 1.77 X 109/ L(0.03-8.25X109) which is much below the national guidelines. It is possible that platelet yield is lower in these donors as during separation, the platelet concentration gradient was influenced by the larger platelet size [1].
Conclusion
This study shows that PRC from HPS donors are not able to keep the international standard and should not be used in the future. Our study is the first one to date to assess the candidacy of those with inherited giant platelet disorders being blood donors. Reference:1. Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author’s Perspective. J Cutan Aesthetic Surg. 2014;7(4):189–97.
Session topic: E-poster
Type: Publication Only
Background
Harris platelet syndrome (HPS) is an autosomal dominant macrothrombocytopenia with mild to moderately severe thrombocytopenia and normal platelet function. HPS has been reported from Indian subcontinent blood donors, especially from the northeastern part of India. Approximately a third of blood donors from state of West Bengal have been found to have HPS.The Indian NACO 2007 guideline recommends one bag of PRC from 350 ml of whole blood should have a platelet count above 3.5 X10 9/l.
Aims
We designed a study to assess whether PRC (platelet rich concentrate) obtained from those with HPS contained adequate platelets as recommended by the Indian guidelines.
Methods
HPS is defined as healthy blood donors having a mean platelet volume (MPV) greater than 12 fL (normal <10 fL) with or without concomitant thrombocytopenia in the absence of any significant past history of bleeding disorder in either themselves or their first degree relatives.In our study, we collected 350 ml of blood from each donor and via Platelet Rich Plasma Separation Method (PRPSM) we separated out PRC and PRBC in separate bags.A CBC was performed at the time of donation and from PRC bag blood collected in EDTA tubes, and analyzed using SYSMEX XP-100.
Results
A total of 28 donors were included. The median age of the donors was 33 years(21-62) and 21 (75%) were male.Pre donation CBC showed a median platelet count of 1.93 ± 0.54 X109/ L (0.99-2.98 X109) and platelet count from PRC showed a median platelet count 1.16 ± 1.77 X 109/ L(0.03-8.25X109) which is much below the national guidelines. It is possible that platelet yield is lower in these donors as during separation, the platelet concentration gradient was influenced by the larger platelet size [1].
| Platelet Indices: | Pre Donation Platelet Indices (n=28) | Platelet indices from PRP (n=28) |
| MPV(Mean) | 13.66 ± 1.99 fl | 10.00 ± 2.02 fl |
| Platelet Count (Median) | 1.93 ± 0.54 X109/L (0.99-2.98 X109/L) | 1.16 ± 1.77 X109/L (0.03-8.25X109/L) |
| PDW(Mean) | 19.80 ± 2.95 | 13.87 ± 4.64 |
| Platelet Biomass(Mean) | 26.91 ± 1.16 | 15.4 ± 9.41 |
Conclusion
This study shows that PRC from HPS donors are not able to keep the international standard and should not be used in the future. Our study is the first one to date to assess the candidacy of those with inherited giant platelet disorders being blood donors. Reference:1. Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author’s Perspective. J Cutan Aesthetic Surg. 2014;7(4):189–97.
Session topic: E-poster
Abstract: PB2232
Type: Publication Only
Background
Harris platelet syndrome (HPS) is an autosomal dominant macrothrombocytopenia with mild to moderately severe thrombocytopenia and normal platelet function. HPS has been reported from Indian subcontinent blood donors, especially from the northeastern part of India. Approximately a third of blood donors from state of West Bengal have been found to have HPS.The Indian NACO 2007 guideline recommends one bag of PRC from 350 ml of whole blood should have a platelet count above 3.5 X10 9/l.
Aims
We designed a study to assess whether PRC (platelet rich concentrate) obtained from those with HPS contained adequate platelets as recommended by the Indian guidelines.
Methods
HPS is defined as healthy blood donors having a mean platelet volume (MPV) greater than 12 fL (normal <10 fL) with or without concomitant thrombocytopenia in the absence of any significant past history of bleeding disorder in either themselves or their first degree relatives.In our study, we collected 350 ml of blood from each donor and via Platelet Rich Plasma Separation Method (PRPSM) we separated out PRC and PRBC in separate bags.A CBC was performed at the time of donation and from PRC bag blood collected in EDTA tubes, and analyzed using SYSMEX XP-100.
Results
A total of 28 donors were included. The median age of the donors was 33 years(21-62) and 21 (75%) were male.Pre donation CBC showed a median platelet count of 1.93 ± 0.54 X109/ L (0.99-2.98 X109) and platelet count from PRC showed a median platelet count 1.16 ± 1.77 X 109/ L(0.03-8.25X109) which is much below the national guidelines. It is possible that platelet yield is lower in these donors as during separation, the platelet concentration gradient was influenced by the larger platelet size [1].
Conclusion
This study shows that PRC from HPS donors are not able to keep the international standard and should not be used in the future. Our study is the first one to date to assess the candidacy of those with inherited giant platelet disorders being blood donors. Reference:1. Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author’s Perspective. J Cutan Aesthetic Surg. 2014;7(4):189–97.
Session topic: E-poster
Type: Publication Only
Background
Harris platelet syndrome (HPS) is an autosomal dominant macrothrombocytopenia with mild to moderately severe thrombocytopenia and normal platelet function. HPS has been reported from Indian subcontinent blood donors, especially from the northeastern part of India. Approximately a third of blood donors from state of West Bengal have been found to have HPS.The Indian NACO 2007 guideline recommends one bag of PRC from 350 ml of whole blood should have a platelet count above 3.5 X10 9/l.
Aims
We designed a study to assess whether PRC (platelet rich concentrate) obtained from those with HPS contained adequate platelets as recommended by the Indian guidelines.
Methods
HPS is defined as healthy blood donors having a mean platelet volume (MPV) greater than 12 fL (normal <10 fL) with or without concomitant thrombocytopenia in the absence of any significant past history of bleeding disorder in either themselves or their first degree relatives.In our study, we collected 350 ml of blood from each donor and via Platelet Rich Plasma Separation Method (PRPSM) we separated out PRC and PRBC in separate bags.A CBC was performed at the time of donation and from PRC bag blood collected in EDTA tubes, and analyzed using SYSMEX XP-100.
Results
A total of 28 donors were included. The median age of the donors was 33 years(21-62) and 21 (75%) were male.Pre donation CBC showed a median platelet count of 1.93 ± 0.54 X109/ L (0.99-2.98 X109) and platelet count from PRC showed a median platelet count 1.16 ± 1.77 X 109/ L(0.03-8.25X109) which is much below the national guidelines. It is possible that platelet yield is lower in these donors as during separation, the platelet concentration gradient was influenced by the larger platelet size [1].
| Platelet Indices: | Pre Donation Platelet Indices (n=28) | Platelet indices from PRP (n=28) |
| MPV(Mean) | 13.66 ± 1.99 fl | 10.00 ± 2.02 fl |
| Platelet Count (Median) | 1.93 ± 0.54 X109/L (0.99-2.98 X109/L) | 1.16 ± 1.77 X109/L (0.03-8.25X109/L) |
| PDW(Mean) | 19.80 ± 2.95 | 13.87 ± 4.64 |
| Platelet Biomass(Mean) | 26.91 ± 1.16 | 15.4 ± 9.41 |
Conclusion
This study shows that PRC from HPS donors are not able to keep the international standard and should not be used in the future. Our study is the first one to date to assess the candidacy of those with inherited giant platelet disorders being blood donors. Reference:1. Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author’s Perspective. J Cutan Aesthetic Surg. 2014;7(4):189–97.
Session topic: E-poster
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