A NEW PROTOCOL FOR OBTAINING PLATELET RICH IN GROWTH FACTORS (PRP). A DESCRIPTIVE STUDY IN 15 PATIENTS AND COMPARISON WITH RESULTS PUBLISHED IN LITERATURE
(Abstract release date: 05/19/16)
EHA Library. Alcaraz Rubio J. 06/09/16; 135127; PB2227
Dr. Jesus Alcaraz Rubio
Contributions
Contributions
Abstract
Abstract: PB2227
Type: Publication Only
Background
The diversity of procedures for obtaining platelet and plasmatic growth factors, the absence of control in most of them and the growing field of clinical application, makes them necessary methods adequately structured, documented, controlled and tested, playable by any author. The present series of clinical cases aims to introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Aims
introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Methods
15 caucasian patients were selected, 8 male and 7 female with age range between 35 and 65, healthy haematologically. The procedure for obtaining the PRP, consisted of a single centrifugation of the blood sample for 30 minutes at 3500 rpm in a angular shaft of 16 tubes centrifuge serie (CEMCON 2) and micropipetting the protein fraction rich in platelet and plasmatic growth factors and cell through open technique under aseptic conditions in horizontal laminar flow hood Grade A at a temperature of 22 ° C, with the use of leuco-platelet or Buffy-coat layer (PRP rich in leukocytes).
Results
No correlation between the amount of concentrated platelets and the amount of growth factors finally obtained was observed. The protocol set forth concentrated levels of platelets and leukocytes approximately 3 to 5 times higher than baseline levels with a predominance of mononuclear. Levels of growth factors from 7-10 times greater than the patient's baseline levels, with little variation in them. The growth factor levels were stable in the blood of each patient within 24 h of treatment between 7 and 9 times higher compared to the previous baseline. Compared with other procedures discussed in the literature; This method achieves concentration between 1.5 and 3 times more platelets in the final product, with a purification of growth factors overall type VEGF and TGF-B clearly superior.
Conclusion
the technique disclosed is more effective since concentrate achieves greater amount of platelets and growth factors and efficient since it maintains a serum protein in these stable sera of patients after 24 hours of administration thereof.
Session topic: E-poster
Keyword(s): Cell processing, Growth factor, Platelet reactivity
Type: Publication Only
Background
The diversity of procedures for obtaining platelet and plasmatic growth factors, the absence of control in most of them and the growing field of clinical application, makes them necessary methods adequately structured, documented, controlled and tested, playable by any author. The present series of clinical cases aims to introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Aims
introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Methods
15 caucasian patients were selected, 8 male and 7 female with age range between 35 and 65, healthy haematologically. The procedure for obtaining the PRP, consisted of a single centrifugation of the blood sample for 30 minutes at 3500 rpm in a angular shaft of 16 tubes centrifuge serie (CEMCON 2) and micropipetting the protein fraction rich in platelet and plasmatic growth factors and cell through open technique under aseptic conditions in horizontal laminar flow hood Grade A at a temperature of 22 ° C, with the use of leuco-platelet or Buffy-coat layer (PRP rich in leukocytes).
Results
No correlation between the amount of concentrated platelets and the amount of growth factors finally obtained was observed. The protocol set forth concentrated levels of platelets and leukocytes approximately 3 to 5 times higher than baseline levels with a predominance of mononuclear. Levels of growth factors from 7-10 times greater than the patient's baseline levels, with little variation in them. The growth factor levels were stable in the blood of each patient within 24 h of treatment between 7 and 9 times higher compared to the previous baseline. Compared with other procedures discussed in the literature; This method achieves concentration between 1.5 and 3 times more platelets in the final product, with a purification of growth factors overall type VEGF and TGF-B clearly superior.
Conclusion
the technique disclosed is more effective since concentrate achieves greater amount of platelets and growth factors and efficient since it maintains a serum protein in these stable sera of patients after 24 hours of administration thereof.
Session topic: E-poster
Keyword(s): Cell processing, Growth factor, Platelet reactivity
Abstract: PB2227
Type: Publication Only
Background
The diversity of procedures for obtaining platelet and plasmatic growth factors, the absence of control in most of them and the growing field of clinical application, makes them necessary methods adequately structured, documented, controlled and tested, playable by any author. The present series of clinical cases aims to introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Aims
introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Methods
15 caucasian patients were selected, 8 male and 7 female with age range between 35 and 65, healthy haematologically. The procedure for obtaining the PRP, consisted of a single centrifugation of the blood sample for 30 minutes at 3500 rpm in a angular shaft of 16 tubes centrifuge serie (CEMCON 2) and micropipetting the protein fraction rich in platelet and plasmatic growth factors and cell through open technique under aseptic conditions in horizontal laminar flow hood Grade A at a temperature of 22 ° C, with the use of leuco-platelet or Buffy-coat layer (PRP rich in leukocytes).
Results
No correlation between the amount of concentrated platelets and the amount of growth factors finally obtained was observed. The protocol set forth concentrated levels of platelets and leukocytes approximately 3 to 5 times higher than baseline levels with a predominance of mononuclear. Levels of growth factors from 7-10 times greater than the patient's baseline levels, with little variation in them. The growth factor levels were stable in the blood of each patient within 24 h of treatment between 7 and 9 times higher compared to the previous baseline. Compared with other procedures discussed in the literature; This method achieves concentration between 1.5 and 3 times more platelets in the final product, with a purification of growth factors overall type VEGF and TGF-B clearly superior.
Conclusion
the technique disclosed is more effective since concentrate achieves greater amount of platelets and growth factors and efficient since it maintains a serum protein in these stable sera of patients after 24 hours of administration thereof.
Session topic: E-poster
Keyword(s): Cell processing, Growth factor, Platelet reactivity
Type: Publication Only
Background
The diversity of procedures for obtaining platelet and plasmatic growth factors, the absence of control in most of them and the growing field of clinical application, makes them necessary methods adequately structured, documented, controlled and tested, playable by any author. The present series of clinical cases aims to introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Aims
introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Methods
15 caucasian patients were selected, 8 male and 7 female with age range between 35 and 65, healthy haematologically. The procedure for obtaining the PRP, consisted of a single centrifugation of the blood sample for 30 minutes at 3500 rpm in a angular shaft of 16 tubes centrifuge serie (CEMCON 2) and micropipetting the protein fraction rich in platelet and plasmatic growth factors and cell through open technique under aseptic conditions in horizontal laminar flow hood Grade A at a temperature of 22 ° C, with the use of leuco-platelet or Buffy-coat layer (PRP rich in leukocytes).
Results
No correlation between the amount of concentrated platelets and the amount of growth factors finally obtained was observed. The protocol set forth concentrated levels of platelets and leukocytes approximately 3 to 5 times higher than baseline levels with a predominance of mononuclear. Levels of growth factors from 7-10 times greater than the patient's baseline levels, with little variation in them. The growth factor levels were stable in the blood of each patient within 24 h of treatment between 7 and 9 times higher compared to the previous baseline. Compared with other procedures discussed in the literature; This method achieves concentration between 1.5 and 3 times more platelets in the final product, with a purification of growth factors overall type VEGF and TGF-B clearly superior.
Conclusion
the technique disclosed is more effective since concentrate achieves greater amount of platelets and growth factors and efficient since it maintains a serum protein in these stable sera of patients after 24 hours of administration thereof.
Session topic: E-poster
Keyword(s): Cell processing, Growth factor, Platelet reactivity
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