INFLUENCE ADRENOCORTICOTROPIC HORMONE ANALOG SEMAX ON FUNCTIONAL ACTIVITY OF BLOOD CELLS DURING IMMOBILIZATION STRESS
(Abstract release date: 05/19/16)
EHA Library. Golubeva М. 06/09/16; 135110; PB2210
Dr. Мария Golubeva
Contributions
Contributions
Abstract
Abstract: PB2210
Type: Publication Only
Background
Bokgraund It is known that many disease states are associated with impaired red blood cell membrane. It is the activity of these blood cells associated normal microcirculation.
At present, special attention is given to researchers semax neuropeptide derived from pituitary adrenocorticotropic hormone. The peptide penetrates the blood-brain barrier and has an impact on a number of functions of the central nervous system. Experiments have shown that peptide can affect blood cells, particularly red blood cells. The red blood cells increase the biochemical reactivity and functional only activated platelets. Change erythrocytes platelet biochemistry is very important for understanding the interaction of blood cells in hemostasis.
It was found that the immobilization of the animal's body are also changes of erythrocyte membrane.
Aims
The aim of this work was to determine the effect of Semax on the functional activity of red blood cells and their interaction with platelets and the influence of these processes immobilization stress.
Methods
To reproduce the experimental stress reaction used immobilization stress by fixing the rats on their backs for 60 minutes. In in vitro experiments semax in the concentration range 10-1 - 10 -10 M is added to a pool of platelet-rich plasma (PRP), or PPP and the mixture washed erythrocytes (e) and change in aggregation was determined by the action of ADP. In experiments in vivo semaks administered intravenously prior to or after the immobilization was determined and osmotic fragility of erythrocytes and the platelet aggregation in normal and by animals immobilized.
Results
In in vitro experiments have shown that, when added to semax pool PRP concentration of 10-10M only caused a significant enhancement of platelet aggregation. Adding PRP mixture of washed red blood cells significantly increased platelet aggregation. When added to the mixture PRP + erythrocyte as a peptide, and directivity of physiological saline retained reactions. In vivo experiments demonstrated that intravenous administration of semax at a dose of 1 mg / ml in a physiological standard had no significant effect on platelet aggregation. In the study of changes in the wholesale electricity market it has shown that semaks increases resistance of the membrane of red blood cells, reducing the percentage of haemolysed cells. The prophylactic administration of semax as well as the administration of the peptide on the background of immobilization stress significantly increased the resistance of erythrocytes to hemolysis.
Conclusion
. Our studies have shown that neuropeptide semaks, on the one hand, no significant effect on hemostasis in a physiological condition, and with another - increased stability of the erythrocyte membrane to the stressor exposure.
Session topic: E-poster
Keyword(s): Erythrocyte, Hemolysis, Peptide, Thrombosis
Type: Publication Only
Background
Bokgraund It is known that many disease states are associated with impaired red blood cell membrane. It is the activity of these blood cells associated normal microcirculation.
At present, special attention is given to researchers semax neuropeptide derived from pituitary adrenocorticotropic hormone. The peptide penetrates the blood-brain barrier and has an impact on a number of functions of the central nervous system. Experiments have shown that peptide can affect blood cells, particularly red blood cells. The red blood cells increase the biochemical reactivity and functional only activated platelets. Change erythrocytes platelet biochemistry is very important for understanding the interaction of blood cells in hemostasis.
It was found that the immobilization of the animal's body are also changes of erythrocyte membrane.
Aims
The aim of this work was to determine the effect of Semax on the functional activity of red blood cells and their interaction with platelets and the influence of these processes immobilization stress.
Methods
To reproduce the experimental stress reaction used immobilization stress by fixing the rats on their backs for 60 minutes. In in vitro experiments semax in the concentration range 10-1 - 10 -10 M is added to a pool of platelet-rich plasma (PRP), or PPP and the mixture washed erythrocytes (e) and change in aggregation was determined by the action of ADP. In experiments in vivo semaks administered intravenously prior to or after the immobilization was determined and osmotic fragility of erythrocytes and the platelet aggregation in normal and by animals immobilized.
Results
In in vitro experiments have shown that, when added to semax pool PRP concentration of 10-10M only caused a significant enhancement of platelet aggregation. Adding PRP mixture of washed red blood cells significantly increased platelet aggregation. When added to the mixture PRP + erythrocyte as a peptide, and directivity of physiological saline retained reactions. In vivo experiments demonstrated that intravenous administration of semax at a dose of 1 mg / ml in a physiological standard had no significant effect on platelet aggregation. In the study of changes in the wholesale electricity market it has shown that semaks increases resistance of the membrane of red blood cells, reducing the percentage of haemolysed cells. The prophylactic administration of semax as well as the administration of the peptide on the background of immobilization stress significantly increased the resistance of erythrocytes to hemolysis.
Conclusion
. Our studies have shown that neuropeptide semaks, on the one hand, no significant effect on hemostasis in a physiological condition, and with another - increased stability of the erythrocyte membrane to the stressor exposure.
Session topic: E-poster
Keyword(s): Erythrocyte, Hemolysis, Peptide, Thrombosis
Abstract: PB2210
Type: Publication Only
Background
Bokgraund It is known that many disease states are associated with impaired red blood cell membrane. It is the activity of these blood cells associated normal microcirculation.
At present, special attention is given to researchers semax neuropeptide derived from pituitary adrenocorticotropic hormone. The peptide penetrates the blood-brain barrier and has an impact on a number of functions of the central nervous system. Experiments have shown that peptide can affect blood cells, particularly red blood cells. The red blood cells increase the biochemical reactivity and functional only activated platelets. Change erythrocytes platelet biochemistry is very important for understanding the interaction of blood cells in hemostasis.
It was found that the immobilization of the animal's body are also changes of erythrocyte membrane.
Aims
The aim of this work was to determine the effect of Semax on the functional activity of red blood cells and their interaction with platelets and the influence of these processes immobilization stress.
Methods
To reproduce the experimental stress reaction used immobilization stress by fixing the rats on their backs for 60 minutes. In in vitro experiments semax in the concentration range 10-1 - 10 -10 M is added to a pool of platelet-rich plasma (PRP), or PPP and the mixture washed erythrocytes (e) and change in aggregation was determined by the action of ADP. In experiments in vivo semaks administered intravenously prior to or after the immobilization was determined and osmotic fragility of erythrocytes and the platelet aggregation in normal and by animals immobilized.
Results
In in vitro experiments have shown that, when added to semax pool PRP concentration of 10-10M only caused a significant enhancement of platelet aggregation. Adding PRP mixture of washed red blood cells significantly increased platelet aggregation. When added to the mixture PRP + erythrocyte as a peptide, and directivity of physiological saline retained reactions. In vivo experiments demonstrated that intravenous administration of semax at a dose of 1 mg / ml in a physiological standard had no significant effect on platelet aggregation. In the study of changes in the wholesale electricity market it has shown that semaks increases resistance of the membrane of red blood cells, reducing the percentage of haemolysed cells. The prophylactic administration of semax as well as the administration of the peptide on the background of immobilization stress significantly increased the resistance of erythrocytes to hemolysis.
Conclusion
. Our studies have shown that neuropeptide semaks, on the one hand, no significant effect on hemostasis in a physiological condition, and with another - increased stability of the erythrocyte membrane to the stressor exposure.
Session topic: E-poster
Keyword(s): Erythrocyte, Hemolysis, Peptide, Thrombosis
Type: Publication Only
Background
Bokgraund It is known that many disease states are associated with impaired red blood cell membrane. It is the activity of these blood cells associated normal microcirculation.
At present, special attention is given to researchers semax neuropeptide derived from pituitary adrenocorticotropic hormone. The peptide penetrates the blood-brain barrier and has an impact on a number of functions of the central nervous system. Experiments have shown that peptide can affect blood cells, particularly red blood cells. The red blood cells increase the biochemical reactivity and functional only activated platelets. Change erythrocytes platelet biochemistry is very important for understanding the interaction of blood cells in hemostasis.
It was found that the immobilization of the animal's body are also changes of erythrocyte membrane.
Aims
The aim of this work was to determine the effect of Semax on the functional activity of red blood cells and their interaction with platelets and the influence of these processes immobilization stress.
Methods
To reproduce the experimental stress reaction used immobilization stress by fixing the rats on their backs for 60 minutes. In in vitro experiments semax in the concentration range 10-1 - 10 -10 M is added to a pool of platelet-rich plasma (PRP), or PPP and the mixture washed erythrocytes (e) and change in aggregation was determined by the action of ADP. In experiments in vivo semaks administered intravenously prior to or after the immobilization was determined and osmotic fragility of erythrocytes and the platelet aggregation in normal and by animals immobilized.
Results
In in vitro experiments have shown that, when added to semax pool PRP concentration of 10-10M only caused a significant enhancement of platelet aggregation. Adding PRP mixture of washed red blood cells significantly increased platelet aggregation. When added to the mixture PRP + erythrocyte as a peptide, and directivity of physiological saline retained reactions. In vivo experiments demonstrated that intravenous administration of semax at a dose of 1 mg / ml in a physiological standard had no significant effect on platelet aggregation. In the study of changes in the wholesale electricity market it has shown that semaks increases resistance of the membrane of red blood cells, reducing the percentage of haemolysed cells. The prophylactic administration of semax as well as the administration of the peptide on the background of immobilization stress significantly increased the resistance of erythrocytes to hemolysis.
Conclusion
. Our studies have shown that neuropeptide semaks, on the one hand, no significant effect on hemostasis in a physiological condition, and with another - increased stability of the erythrocyte membrane to the stressor exposure.
Session topic: E-poster
Keyword(s): Erythrocyte, Hemolysis, Peptide, Thrombosis
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