THE INFLUENCE OF HAPLOTYPE HLA-C(C1/X,C2/X) IN EVOLUTION AFTER HSCT
(Abstract release date: 05/19/16)
EHA Library. Bratu D. 06/09/16; 135093; PB2193
Dr. Denisa Bratu
Contributions
Contributions
Abstract
Abstract: PB2193
Type: Publication Only
Background
Haplotypes of patients with acute leukemia like ligands or not for inhibitories and activatories donors KIR allele in HSCT are subjects of studies in a few specialised clinics ; it is an evidence that haplotypes can be protective or not against postHSCT complication,easier to try to demonstrate at patients with genoidentical donors.
Aims
Haplotype HLA-C(C1/X,C2/X),homozygot and heterozygot variants(HLA-C1/C1,HLA-C1/C2,HLA-C2/C2) seems to have influence at patients with acute leukemia after HSCT.
Methods
Eighteen pairs patients-donors are evaluated :patients with acute leukemia,lymphoblastic and non-lymphoblastic and their genoidentics donors.One patient have HLA-C1/C1 haplotype,seven HLA-C1/C2,ten HLA-C2/C2 .Following the impact of inhibitory KIR2DL1, like ligand for C2,KIR2DL2,KIR2DL3 for C1, activatory KIR2DS1 for C2,KIR2DS4 for C1,on survival and complication development,we proved the protective effect of presence of HLA-C2/X haplotype,HLA-C1/X,respectively.The source of HSCT was PBSC.The method used was PCR-SSP (Innotrain DIAGNOSTIK GMBH,Dynal BIOTECH PEL-FREEZE )The complications like graft versus host disease acute and chronic,relapse,TMA and the recovery with leucocytes and thrombocytes are followed.
Results
Presence of HLA-C1 haplotype is protective in presence of KIR2DS4(activatory allele) with statistical significance for thrombocyte recovery but no influence against aGVHD and relapse; in presence of KIR2DL2,KIR2DL3(inhibitory allele) against relapse,TMA,a/cGVHD, leucocytes and thrombocytes recovery,without statistical significance.HLA-C2, in presence of KIR2DS1,and also in presence of KIR2DL1 protective against TMA and relapse,cGVHD,also leucocyte and thrombocytes recovery,without statistical significance,except aGVHD (no influence)and observation for absence HLA-C2 with protective effect against relapse(„missing ligand”).
Conclusion
Presence of HLA-C1/X,HLA-C2/X4 improve survival and offer minor protection against most complication at patients with acute leukemia and related donors with 100% allele match,in presence of both types of KIR alleles .
Session topic: E-poster
Keyword(s): HLA, HSCT, KIR
Type: Publication Only
Background
Haplotypes of patients with acute leukemia like ligands or not for inhibitories and activatories donors KIR allele in HSCT are subjects of studies in a few specialised clinics ; it is an evidence that haplotypes can be protective or not against postHSCT complication,easier to try to demonstrate at patients with genoidentical donors.
Aims
Haplotype HLA-C(C1/X,C2/X),homozygot and heterozygot variants(HLA-C1/C1,HLA-C1/C2,HLA-C2/C2) seems to have influence at patients with acute leukemia after HSCT.
Methods
Eighteen pairs patients-donors are evaluated :patients with acute leukemia,lymphoblastic and non-lymphoblastic and their genoidentics donors.One patient have HLA-C1/C1 haplotype,seven HLA-C1/C2,ten HLA-C2/C2 .Following the impact of inhibitory KIR2DL1, like ligand for C2,KIR2DL2,KIR2DL3 for C1, activatory KIR2DS1 for C2,KIR2DS4 for C1,on survival and complication development,we proved the protective effect of presence of HLA-C2/X haplotype,HLA-C1/X,respectively.The source of HSCT was PBSC.The method used was PCR-SSP (Innotrain DIAGNOSTIK GMBH,Dynal BIOTECH PEL-FREEZE )The complications like graft versus host disease acute and chronic,relapse,TMA and the recovery with leucocytes and thrombocytes are followed.
Results
Presence of HLA-C1 haplotype is protective in presence of KIR2DS4(activatory allele) with statistical significance for thrombocyte recovery but no influence against aGVHD and relapse; in presence of KIR2DL2,KIR2DL3(inhibitory allele) against relapse,TMA,a/cGVHD, leucocytes and thrombocytes recovery,without statistical significance.HLA-C2, in presence of KIR2DS1,and also in presence of KIR2DL1 protective against TMA and relapse,cGVHD,also leucocyte and thrombocytes recovery,without statistical significance,except aGVHD (no influence)and observation for absence HLA-C2 with protective effect against relapse(„missing ligand”).
Conclusion
Presence of HLA-C1/X,HLA-C2/X4 improve survival and offer minor protection against most complication at patients with acute leukemia and related donors with 100% allele match,in presence of both types of KIR alleles .
Session topic: E-poster
Keyword(s): HLA, HSCT, KIR
Abstract: PB2193
Type: Publication Only
Background
Haplotypes of patients with acute leukemia like ligands or not for inhibitories and activatories donors KIR allele in HSCT are subjects of studies in a few specialised clinics ; it is an evidence that haplotypes can be protective or not against postHSCT complication,easier to try to demonstrate at patients with genoidentical donors.
Aims
Haplotype HLA-C(C1/X,C2/X),homozygot and heterozygot variants(HLA-C1/C1,HLA-C1/C2,HLA-C2/C2) seems to have influence at patients with acute leukemia after HSCT.
Methods
Eighteen pairs patients-donors are evaluated :patients with acute leukemia,lymphoblastic and non-lymphoblastic and their genoidentics donors.One patient have HLA-C1/C1 haplotype,seven HLA-C1/C2,ten HLA-C2/C2 .Following the impact of inhibitory KIR2DL1, like ligand for C2,KIR2DL2,KIR2DL3 for C1, activatory KIR2DS1 for C2,KIR2DS4 for C1,on survival and complication development,we proved the protective effect of presence of HLA-C2/X haplotype,HLA-C1/X,respectively.The source of HSCT was PBSC.The method used was PCR-SSP (Innotrain DIAGNOSTIK GMBH,Dynal BIOTECH PEL-FREEZE )The complications like graft versus host disease acute and chronic,relapse,TMA and the recovery with leucocytes and thrombocytes are followed.
Results
Presence of HLA-C1 haplotype is protective in presence of KIR2DS4(activatory allele) with statistical significance for thrombocyte recovery but no influence against aGVHD and relapse; in presence of KIR2DL2,KIR2DL3(inhibitory allele) against relapse,TMA,a/cGVHD, leucocytes and thrombocytes recovery,without statistical significance.HLA-C2, in presence of KIR2DS1,and also in presence of KIR2DL1 protective against TMA and relapse,cGVHD,also leucocyte and thrombocytes recovery,without statistical significance,except aGVHD (no influence)and observation for absence HLA-C2 with protective effect against relapse(„missing ligand”).
Conclusion
Presence of HLA-C1/X,HLA-C2/X4 improve survival and offer minor protection against most complication at patients with acute leukemia and related donors with 100% allele match,in presence of both types of KIR alleles .
Session topic: E-poster
Keyword(s): HLA, HSCT, KIR
Type: Publication Only
Background
Haplotypes of patients with acute leukemia like ligands or not for inhibitories and activatories donors KIR allele in HSCT are subjects of studies in a few specialised clinics ; it is an evidence that haplotypes can be protective or not against postHSCT complication,easier to try to demonstrate at patients with genoidentical donors.
Aims
Haplotype HLA-C(C1/X,C2/X),homozygot and heterozygot variants(HLA-C1/C1,HLA-C1/C2,HLA-C2/C2) seems to have influence at patients with acute leukemia after HSCT.
Methods
Eighteen pairs patients-donors are evaluated :patients with acute leukemia,lymphoblastic and non-lymphoblastic and their genoidentics donors.One patient have HLA-C1/C1 haplotype,seven HLA-C1/C2,ten HLA-C2/C2 .Following the impact of inhibitory KIR2DL1, like ligand for C2,KIR2DL2,KIR2DL3 for C1, activatory KIR2DS1 for C2,KIR2DS4 for C1,on survival and complication development,we proved the protective effect of presence of HLA-C2/X haplotype,HLA-C1/X,respectively.The source of HSCT was PBSC.The method used was PCR-SSP (Innotrain DIAGNOSTIK GMBH,Dynal BIOTECH PEL-FREEZE )The complications like graft versus host disease acute and chronic,relapse,TMA and the recovery with leucocytes and thrombocytes are followed.
Results
Presence of HLA-C1 haplotype is protective in presence of KIR2DS4(activatory allele) with statistical significance for thrombocyte recovery but no influence against aGVHD and relapse; in presence of KIR2DL2,KIR2DL3(inhibitory allele) against relapse,TMA,a/cGVHD, leucocytes and thrombocytes recovery,without statistical significance.HLA-C2, in presence of KIR2DS1,and also in presence of KIR2DL1 protective against TMA and relapse,cGVHD,also leucocyte and thrombocytes recovery,without statistical significance,except aGVHD (no influence)and observation for absence HLA-C2 with protective effect against relapse(„missing ligand”).
Conclusion
Presence of HLA-C1/X,HLA-C2/X4 improve survival and offer minor protection against most complication at patients with acute leukemia and related donors with 100% allele match,in presence of both types of KIR alleles .
Session topic: E-poster
Keyword(s): HLA, HSCT, KIR
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