PERFORMANCE EVALUATION OF RETICULOCYTE COUNT ADOPTING NEW CALIBRATOR XN CAL ON THE SYSMEX XN SERIES.
(Abstract release date: 05/19/16)
EHA Library. Kim H. 06/09/16; 135023; PB2123
Prof. Dr. Hye Ryoun Kim
Contributions
Contributions
Abstract
Abstract: PB2123
Type: Publication Only
Background
Reticulocyte counts have been known as an accurate index of erythropoietic activity. Currently, automated hematology analyzer could measure the reticulocyte count with increased precision and accuracy. The newly introduced Sysmex XN (Sysmex Corporation, Kobe, Japan) has been introduced calibration of reticulocyte count that was not applied by the previous hematology analyzer XE series.
Aims
to clarify the effect of calibration, we evaluated the performance of reticulocyte counting with the Sysmex XN and XE series.
Methods
The XN-9000 was calibrated with new calibrator XN CAL and total 40 blood samples were analyzed to evaluate accuracy of reticulocyte count. The results from the XN-9000 and previous hematology analyzer (XE-2100) were compared with those obtained by manual reticulocyte count as reference method. The precision profiles of the XN-9000 were also evaluated with quality control materials according to Clinical & Laboratory Standards Institute (CLSI) document EP05-A3.
Results
There were perfectly matched results between the XN-9000 and reference method, however the XE-2100 consistently gave significantly lower values compared with reference method. In precision evaluation, the XN-9000 showed impressively improved precision profiles than those of the XE-2100.
Conclusion
Our study has shown that correlation between the two instruments are good, but the bias of reticulocyte count significantly exists in the XE-2100, thus the effort of calibrating reticulocyte is critical to minimize instrument-to-instrument difference. By calibrating reticulocyte count, the XN series could provide more accurate results of reticulocyte count, as well as improved precision.
Session topic: E-poster
Keyword(s): Automation, Quality control, Reticulocyte
Type: Publication Only
Background
Reticulocyte counts have been known as an accurate index of erythropoietic activity. Currently, automated hematology analyzer could measure the reticulocyte count with increased precision and accuracy. The newly introduced Sysmex XN (Sysmex Corporation, Kobe, Japan) has been introduced calibration of reticulocyte count that was not applied by the previous hematology analyzer XE series.
Aims
to clarify the effect of calibration, we evaluated the performance of reticulocyte counting with the Sysmex XN and XE series.
Methods
The XN-9000 was calibrated with new calibrator XN CAL and total 40 blood samples were analyzed to evaluate accuracy of reticulocyte count. The results from the XN-9000 and previous hematology analyzer (XE-2100) were compared with those obtained by manual reticulocyte count as reference method. The precision profiles of the XN-9000 were also evaluated with quality control materials according to Clinical & Laboratory Standards Institute (CLSI) document EP05-A3.
Results
There were perfectly matched results between the XN-9000 and reference method, however the XE-2100 consistently gave significantly lower values compared with reference method. In precision evaluation, the XN-9000 showed impressively improved precision profiles than those of the XE-2100.
Conclusion
Our study has shown that correlation between the two instruments are good, but the bias of reticulocyte count significantly exists in the XE-2100, thus the effort of calibrating reticulocyte is critical to minimize instrument-to-instrument difference. By calibrating reticulocyte count, the XN series could provide more accurate results of reticulocyte count, as well as improved precision.
Session topic: E-poster
Keyword(s): Automation, Quality control, Reticulocyte
Abstract: PB2123
Type: Publication Only
Background
Reticulocyte counts have been known as an accurate index of erythropoietic activity. Currently, automated hematology analyzer could measure the reticulocyte count with increased precision and accuracy. The newly introduced Sysmex XN (Sysmex Corporation, Kobe, Japan) has been introduced calibration of reticulocyte count that was not applied by the previous hematology analyzer XE series.
Aims
to clarify the effect of calibration, we evaluated the performance of reticulocyte counting with the Sysmex XN and XE series.
Methods
The XN-9000 was calibrated with new calibrator XN CAL and total 40 blood samples were analyzed to evaluate accuracy of reticulocyte count. The results from the XN-9000 and previous hematology analyzer (XE-2100) were compared with those obtained by manual reticulocyte count as reference method. The precision profiles of the XN-9000 were also evaluated with quality control materials according to Clinical & Laboratory Standards Institute (CLSI) document EP05-A3.
Results
There were perfectly matched results between the XN-9000 and reference method, however the XE-2100 consistently gave significantly lower values compared with reference method. In precision evaluation, the XN-9000 showed impressively improved precision profiles than those of the XE-2100.
Conclusion
Our study has shown that correlation between the two instruments are good, but the bias of reticulocyte count significantly exists in the XE-2100, thus the effort of calibrating reticulocyte is critical to minimize instrument-to-instrument difference. By calibrating reticulocyte count, the XN series could provide more accurate results of reticulocyte count, as well as improved precision.
Session topic: E-poster
Keyword(s): Automation, Quality control, Reticulocyte
Type: Publication Only
Background
Reticulocyte counts have been known as an accurate index of erythropoietic activity. Currently, automated hematology analyzer could measure the reticulocyte count with increased precision and accuracy. The newly introduced Sysmex XN (Sysmex Corporation, Kobe, Japan) has been introduced calibration of reticulocyte count that was not applied by the previous hematology analyzer XE series.
Aims
to clarify the effect of calibration, we evaluated the performance of reticulocyte counting with the Sysmex XN and XE series.
Methods
The XN-9000 was calibrated with new calibrator XN CAL and total 40 blood samples were analyzed to evaluate accuracy of reticulocyte count. The results from the XN-9000 and previous hematology analyzer (XE-2100) were compared with those obtained by manual reticulocyte count as reference method. The precision profiles of the XN-9000 were also evaluated with quality control materials according to Clinical & Laboratory Standards Institute (CLSI) document EP05-A3.
Results
There were perfectly matched results between the XN-9000 and reference method, however the XE-2100 consistently gave significantly lower values compared with reference method. In precision evaluation, the XN-9000 showed impressively improved precision profiles than those of the XE-2100.
Conclusion
Our study has shown that correlation between the two instruments are good, but the bias of reticulocyte count significantly exists in the XE-2100, thus the effort of calibrating reticulocyte is critical to minimize instrument-to-instrument difference. By calibrating reticulocyte count, the XN series could provide more accurate results of reticulocyte count, as well as improved precision.
Session topic: E-poster
Keyword(s): Automation, Quality control, Reticulocyte
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