PROSPECTIVE COMPARATIVE STUDY BETWEEN CYTOLOGY COUPLED TO THE FLOW CYTOMETRY BY FINE NEEDLE ASPIRATION AND HISTOLOGY IN THE DIAGNOSIS AND CLASSIFICATION OF LARGE B CELL LYMPHOMA
(Abstract release date: 05/19/16)
EHA Library. Mohamed Rafik A. 06/09/16; 134953; PB2053
Dr. ABBADI Mohamed Rafik
Contributions
Contributions
Abstract
Abstract: PB2053
Type: Publication Only
Background
Lymphoma B cells can be studied by flow cytometry (FCM) using fine needle aspiration (FNA) on suspensions of lymph node or extra nodal samples. The cytological analysis and the use of special immunophenotypic markers allows orientation of diagnosis.
Aims
Importance of the FCM coupled to morphology in ambulatory diagnosis of B-cell lymphoma.
Methods
Cases of NHL that were diagnosed primarily by FNA at our instituation between january 2010 and december 2015. 180 samples were studied (140 lymph nodes and 40 extra nodal samples). 134 samples from the 121 evaluable patients referred for suspicion of lymphoma were studied: these patients were, a cytological study and a fine needle aspiration coupled with FCM. 10 antibodies were tested. Large B cell lymphoma was diagnosed in 37 patients (28%) of 33 lymph nodes, 2 pleural fluids and 2 subcutaneous masses. Histological examination with immunohistochemistry were performed in 31 patients : 23 lymph nodes, 1 bone marrow, 2 tonsillectomies, 2 vaginal, 1 skin, 1 bronchial and 1 nasal mass.
Results
In our study the mean age was 60 years, with a male predominance (Ratio =1.8).- On the cytological analysis: all smears were monomorphic in most cases, with large cells appearance, regular nucleus and basophile cytoplasm.- On CMF analysis, the diagnosis was de novo in 31 patients and at relapse in 6 patients. lymph node involvement in 33 cases (89%), extra nodel in 4 cases (11%).The markers (CD20, CD22, CD79b) positive in 100% of cases; CD5 positive in 45% with low expression. CD23 and CD10 were negatives in 90% of cases. Isotype restriction was lambda in 83%.-Histological analysis: 26 cases Diffuse Large B-cell lymphoma (DLBCL), 3 cases B small-cell NHL, 1 case reactive hyperplasia (RH) and 1 case adenocarcinoma. Immunohistochemistry: CD20 was positive in all DLBCL.The correlation between the FCM and histology in the diagnosis of de novo large B cells NHL was 84%.
Conclusion
FCM enhances diagnostic ability of FNA cytology, playing a crucial role in a rapid and accurate differential diagnosis between RH, B-NHL and T-NHL. In addition, immunophenotyping of FNA samples contributes to a more precise sub classification of B-NHL when combined with histopathology.
Session topic: E-poster
Keyword(s): B cell lymphoma
Type: Publication Only
Background
Lymphoma B cells can be studied by flow cytometry (FCM) using fine needle aspiration (FNA) on suspensions of lymph node or extra nodal samples. The cytological analysis and the use of special immunophenotypic markers allows orientation of diagnosis.
Aims
Importance of the FCM coupled to morphology in ambulatory diagnosis of B-cell lymphoma.
Methods
Cases of NHL that were diagnosed primarily by FNA at our instituation between january 2010 and december 2015. 180 samples were studied (140 lymph nodes and 40 extra nodal samples). 134 samples from the 121 evaluable patients referred for suspicion of lymphoma were studied: these patients were, a cytological study and a fine needle aspiration coupled with FCM. 10 antibodies were tested. Large B cell lymphoma was diagnosed in 37 patients (28%) of 33 lymph nodes, 2 pleural fluids and 2 subcutaneous masses. Histological examination with immunohistochemistry were performed in 31 patients : 23 lymph nodes, 1 bone marrow, 2 tonsillectomies, 2 vaginal, 1 skin, 1 bronchial and 1 nasal mass.
Results
In our study the mean age was 60 years, with a male predominance (Ratio =1.8).- On the cytological analysis: all smears were monomorphic in most cases, with large cells appearance, regular nucleus and basophile cytoplasm.- On CMF analysis, the diagnosis was de novo in 31 patients and at relapse in 6 patients. lymph node involvement in 33 cases (89%), extra nodel in 4 cases (11%).The markers (CD20, CD22, CD79b) positive in 100% of cases; CD5 positive in 45% with low expression. CD23 and CD10 were negatives in 90% of cases. Isotype restriction was lambda in 83%.-Histological analysis: 26 cases Diffuse Large B-cell lymphoma (DLBCL), 3 cases B small-cell NHL, 1 case reactive hyperplasia (RH) and 1 case adenocarcinoma. Immunohistochemistry: CD20 was positive in all DLBCL.The correlation between the FCM and histology in the diagnosis of de novo large B cells NHL was 84%.
Conclusion
FCM enhances diagnostic ability of FNA cytology, playing a crucial role in a rapid and accurate differential diagnosis between RH, B-NHL and T-NHL. In addition, immunophenotyping of FNA samples contributes to a more precise sub classification of B-NHL when combined with histopathology.
Session topic: E-poster
Keyword(s): B cell lymphoma
Abstract: PB2053
Type: Publication Only
Background
Lymphoma B cells can be studied by flow cytometry (FCM) using fine needle aspiration (FNA) on suspensions of lymph node or extra nodal samples. The cytological analysis and the use of special immunophenotypic markers allows orientation of diagnosis.
Aims
Importance of the FCM coupled to morphology in ambulatory diagnosis of B-cell lymphoma.
Methods
Cases of NHL that were diagnosed primarily by FNA at our instituation between january 2010 and december 2015. 180 samples were studied (140 lymph nodes and 40 extra nodal samples). 134 samples from the 121 evaluable patients referred for suspicion of lymphoma were studied: these patients were, a cytological study and a fine needle aspiration coupled with FCM. 10 antibodies were tested. Large B cell lymphoma was diagnosed in 37 patients (28%) of 33 lymph nodes, 2 pleural fluids and 2 subcutaneous masses. Histological examination with immunohistochemistry were performed in 31 patients : 23 lymph nodes, 1 bone marrow, 2 tonsillectomies, 2 vaginal, 1 skin, 1 bronchial and 1 nasal mass.
Results
In our study the mean age was 60 years, with a male predominance (Ratio =1.8).- On the cytological analysis: all smears were monomorphic in most cases, with large cells appearance, regular nucleus and basophile cytoplasm.- On CMF analysis, the diagnosis was de novo in 31 patients and at relapse in 6 patients. lymph node involvement in 33 cases (89%), extra nodel in 4 cases (11%).The markers (CD20, CD22, CD79b) positive in 100% of cases; CD5 positive in 45% with low expression. CD23 and CD10 were negatives in 90% of cases. Isotype restriction was lambda in 83%.-Histological analysis: 26 cases Diffuse Large B-cell lymphoma (DLBCL), 3 cases B small-cell NHL, 1 case reactive hyperplasia (RH) and 1 case adenocarcinoma. Immunohistochemistry: CD20 was positive in all DLBCL.The correlation between the FCM and histology in the diagnosis of de novo large B cells NHL was 84%.
Conclusion
FCM enhances diagnostic ability of FNA cytology, playing a crucial role in a rapid and accurate differential diagnosis between RH, B-NHL and T-NHL. In addition, immunophenotyping of FNA samples contributes to a more precise sub classification of B-NHL when combined with histopathology.
Session topic: E-poster
Keyword(s): B cell lymphoma
Type: Publication Only
Background
Lymphoma B cells can be studied by flow cytometry (FCM) using fine needle aspiration (FNA) on suspensions of lymph node or extra nodal samples. The cytological analysis and the use of special immunophenotypic markers allows orientation of diagnosis.
Aims
Importance of the FCM coupled to morphology in ambulatory diagnosis of B-cell lymphoma.
Methods
Cases of NHL that were diagnosed primarily by FNA at our instituation between january 2010 and december 2015. 180 samples were studied (140 lymph nodes and 40 extra nodal samples). 134 samples from the 121 evaluable patients referred for suspicion of lymphoma were studied: these patients were, a cytological study and a fine needle aspiration coupled with FCM. 10 antibodies were tested. Large B cell lymphoma was diagnosed in 37 patients (28%) of 33 lymph nodes, 2 pleural fluids and 2 subcutaneous masses. Histological examination with immunohistochemistry were performed in 31 patients : 23 lymph nodes, 1 bone marrow, 2 tonsillectomies, 2 vaginal, 1 skin, 1 bronchial and 1 nasal mass.
Results
In our study the mean age was 60 years, with a male predominance (Ratio =1.8).- On the cytological analysis: all smears were monomorphic in most cases, with large cells appearance, regular nucleus and basophile cytoplasm.- On CMF analysis, the diagnosis was de novo in 31 patients and at relapse in 6 patients. lymph node involvement in 33 cases (89%), extra nodel in 4 cases (11%).The markers (CD20, CD22, CD79b) positive in 100% of cases; CD5 positive in 45% with low expression. CD23 and CD10 were negatives in 90% of cases. Isotype restriction was lambda in 83%.-Histological analysis: 26 cases Diffuse Large B-cell lymphoma (DLBCL), 3 cases B small-cell NHL, 1 case reactive hyperplasia (RH) and 1 case adenocarcinoma. Immunohistochemistry: CD20 was positive in all DLBCL.The correlation between the FCM and histology in the diagnosis of de novo large B cells NHL was 84%.
Conclusion
FCM enhances diagnostic ability of FNA cytology, playing a crucial role in a rapid and accurate differential diagnosis between RH, B-NHL and T-NHL. In addition, immunophenotyping of FNA samples contributes to a more precise sub classification of B-NHL when combined with histopathology.
Session topic: E-poster
Keyword(s): B cell lymphoma
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