EFFECTS OF MICRORNA-21 ON APOPTOSIS BY REGULATING THE EXPRESSION OF PTEN IN DIFFUSE LARGE B-CELL LYMPHOMA
(Abstract release date: 05/19/16)
EHA Library. Fu R. 06/09/16; 134952; PB2052
Dr. Rong Fu
Contributions
Contributions
Abstract
Abstract: PB2052
Type: Publication Only
Background
Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy and the most common subtype of non-Hodgkin’s lymphoma in China. We found that plasma miR-21 level was significantly higher in B-cell lymphoma. However, the exact contribution of miR-21 in DLBCL remains unknown.
Aims
To determine the function and mechanism of miR-21 in DLBCL.
Methods
miR-21 and phosphatase and tensin homolog (PTEN) expressions were examined through real-time PCR and immunohistochemical methods. Moreover, the effects of antisense oligonucleotide (ASO) targeting miR-21 (ASO-21) were observed in DLCBL cell line.
Results
Results showed that miR-21 expressions in cell line and tissues of patients were significantly higher than those in normal controls, which were inversely correlated with PTEN expression. MiR-21 expression was significantly higher in stage III/IV patients than in stage I/II patients. After downregulating the miR-21 expression, apoptosis of DLBCL cells increased and PTEN protein was upregulated.
Conclusion
These results suggested that miR-21 affects apoptosis of lymphoma cells by regulating the expression of PTEN in DLBCL, which may be associated with increased poor prognosis for DLBCL patients and represents a useful approach for DLBCL treatment.
Session topic: E-poster
Keyword(s): DLBCL, PTEN
Type: Publication Only
Background
Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy and the most common subtype of non-Hodgkin’s lymphoma in China. We found that plasma miR-21 level was significantly higher in B-cell lymphoma. However, the exact contribution of miR-21 in DLBCL remains unknown.
Aims
To determine the function and mechanism of miR-21 in DLBCL.
Methods
miR-21 and phosphatase and tensin homolog (PTEN) expressions were examined through real-time PCR and immunohistochemical methods. Moreover, the effects of antisense oligonucleotide (ASO) targeting miR-21 (ASO-21) were observed in DLCBL cell line.
Results
Results showed that miR-21 expressions in cell line and tissues of patients were significantly higher than those in normal controls, which were inversely correlated with PTEN expression. MiR-21 expression was significantly higher in stage III/IV patients than in stage I/II patients. After downregulating the miR-21 expression, apoptosis of DLBCL cells increased and PTEN protein was upregulated.
Conclusion
These results suggested that miR-21 affects apoptosis of lymphoma cells by regulating the expression of PTEN in DLBCL, which may be associated with increased poor prognosis for DLBCL patients and represents a useful approach for DLBCL treatment.
Session topic: E-poster
Keyword(s): DLBCL, PTEN
Abstract: PB2052
Type: Publication Only
Background
Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy and the most common subtype of non-Hodgkin’s lymphoma in China. We found that plasma miR-21 level was significantly higher in B-cell lymphoma. However, the exact contribution of miR-21 in DLBCL remains unknown.
Aims
To determine the function and mechanism of miR-21 in DLBCL.
Methods
miR-21 and phosphatase and tensin homolog (PTEN) expressions were examined through real-time PCR and immunohistochemical methods. Moreover, the effects of antisense oligonucleotide (ASO) targeting miR-21 (ASO-21) were observed in DLCBL cell line.
Results
Results showed that miR-21 expressions in cell line and tissues of patients were significantly higher than those in normal controls, which were inversely correlated with PTEN expression. MiR-21 expression was significantly higher in stage III/IV patients than in stage I/II patients. After downregulating the miR-21 expression, apoptosis of DLBCL cells increased and PTEN protein was upregulated.
Conclusion
These results suggested that miR-21 affects apoptosis of lymphoma cells by regulating the expression of PTEN in DLBCL, which may be associated with increased poor prognosis for DLBCL patients and represents a useful approach for DLBCL treatment.
Session topic: E-poster
Keyword(s): DLBCL, PTEN
Type: Publication Only
Background
Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy and the most common subtype of non-Hodgkin’s lymphoma in China. We found that plasma miR-21 level was significantly higher in B-cell lymphoma. However, the exact contribution of miR-21 in DLBCL remains unknown.
Aims
To determine the function and mechanism of miR-21 in DLBCL.
Methods
miR-21 and phosphatase and tensin homolog (PTEN) expressions were examined through real-time PCR and immunohistochemical methods. Moreover, the effects of antisense oligonucleotide (ASO) targeting miR-21 (ASO-21) were observed in DLCBL cell line.
Results
Results showed that miR-21 expressions in cell line and tissues of patients were significantly higher than those in normal controls, which were inversely correlated with PTEN expression. MiR-21 expression was significantly higher in stage III/IV patients than in stage I/II patients. After downregulating the miR-21 expression, apoptosis of DLBCL cells increased and PTEN protein was upregulated.
Conclusion
These results suggested that miR-21 affects apoptosis of lymphoma cells by regulating the expression of PTEN in DLBCL, which may be associated with increased poor prognosis for DLBCL patients and represents a useful approach for DLBCL treatment.
Session topic: E-poster
Keyword(s): DLBCL, PTEN
{{ help_message }}
{{filter}}