PROGRESS IN THE DIAGNOSIS OF PRIMARY CENTRAL NERVOUS SYSTEM LYMPHOMA
(Abstract release date: 05/19/16)
EHA Library. Debliquis A. 06/09/16; 134949; PB2049
Dr. Agathe Debliquis
Contributions
Contributions
Abstract
Abstract: PB2049
Type: Publication Only
Background
Central nervous system lymphomas are aggressive tumors requiring a prompt diagnosis for successful treatment. Stereotactic brain biopsy remains the standard procedure, but the time needed for histopathology is usually over 2 days.
Aims
During stereotactic brain biopsy, the trocar is rinsed with saline solution and we evaluated the contribution of cytomorphology and flow cytometry of this rinse fluid usually removed.
Methods
Eighteen patients with suspected localized brain lymphoma underwent stereotactic brain biopsy. Brain biopsy tissue sample and/or brain biopsy rinse fluid were analyzed by cytomorphology combined with flow cytometry. Eleven patients had both types of sample, four patients had only brain biopsy tissue sample, and three patients had only brain biopsy rinse fluid. Histopathology was used as a reference.
Results
Histopathology characterized ten diffuse large B-cell lymphomas and eight other diseases: three glioblastomas, one necrosis, one Erdheim-Chester disease, one cerebral vasculitis, one stroke and one anaplastic astrocytoma.Cytomorphology and flow cytometry showed lymphoma cells in nine out of the ten lymphomas. Three cytomorphology or flow cytometry negative results were reported for lymphomas in tissue samples due to low cellularity and biopsy sample conditioning. No lymphomatous cells were found by cytomorphology or flow cytometry in the eight other diseases. Rinse fluid results were consistent with histology in all cases studied (sensitivity and specificity, 100%). All lymphomas characterized by flow cytometry were: CD5−, CD20+, Ki67 high and CD10 was expressed in six out of the nine cases as in histopathology. CD44, CD184, BCL2, and BCL6 showed variable expression.The median time to result was 4.5 days (range, 2–10) for histopathology, while 5 h (range, 3–20) were required for both cytomorphology and flow cytometry.
Conclusion
Brain biopsy rinse fluid alleviates problems of tissue sample distribution compared to tissue sample. Its analysis performs diagnosis of B-cell lymphoma in a few hours and, associated with histopathology, allows a multidisciplinary diagnosis. This study shows that cytomorphology combined with flow cytometry on brain biospy rinse fluid is a new, fast, and useful strategy.
Session topic: E-poster
Keyword(s): CNS lymphoma, Diagnosis, Flow cytometry
Type: Publication Only
Background
Central nervous system lymphomas are aggressive tumors requiring a prompt diagnosis for successful treatment. Stereotactic brain biopsy remains the standard procedure, but the time needed for histopathology is usually over 2 days.
Aims
During stereotactic brain biopsy, the trocar is rinsed with saline solution and we evaluated the contribution of cytomorphology and flow cytometry of this rinse fluid usually removed.
Methods
Eighteen patients with suspected localized brain lymphoma underwent stereotactic brain biopsy. Brain biopsy tissue sample and/or brain biopsy rinse fluid were analyzed by cytomorphology combined with flow cytometry. Eleven patients had both types of sample, four patients had only brain biopsy tissue sample, and three patients had only brain biopsy rinse fluid. Histopathology was used as a reference.
Results
Histopathology characterized ten diffuse large B-cell lymphomas and eight other diseases: three glioblastomas, one necrosis, one Erdheim-Chester disease, one cerebral vasculitis, one stroke and one anaplastic astrocytoma.Cytomorphology and flow cytometry showed lymphoma cells in nine out of the ten lymphomas. Three cytomorphology or flow cytometry negative results were reported for lymphomas in tissue samples due to low cellularity and biopsy sample conditioning. No lymphomatous cells were found by cytomorphology or flow cytometry in the eight other diseases. Rinse fluid results were consistent with histology in all cases studied (sensitivity and specificity, 100%). All lymphomas characterized by flow cytometry were: CD5−, CD20+, Ki67 high and CD10 was expressed in six out of the nine cases as in histopathology. CD44, CD184, BCL2, and BCL6 showed variable expression.The median time to result was 4.5 days (range, 2–10) for histopathology, while 5 h (range, 3–20) were required for both cytomorphology and flow cytometry.
Conclusion
Brain biopsy rinse fluid alleviates problems of tissue sample distribution compared to tissue sample. Its analysis performs diagnosis of B-cell lymphoma in a few hours and, associated with histopathology, allows a multidisciplinary diagnosis. This study shows that cytomorphology combined with flow cytometry on brain biospy rinse fluid is a new, fast, and useful strategy.
Session topic: E-poster
Keyword(s): CNS lymphoma, Diagnosis, Flow cytometry
Abstract: PB2049
Type: Publication Only
Background
Central nervous system lymphomas are aggressive tumors requiring a prompt diagnosis for successful treatment. Stereotactic brain biopsy remains the standard procedure, but the time needed for histopathology is usually over 2 days.
Aims
During stereotactic brain biopsy, the trocar is rinsed with saline solution and we evaluated the contribution of cytomorphology and flow cytometry of this rinse fluid usually removed.
Methods
Eighteen patients with suspected localized brain lymphoma underwent stereotactic brain biopsy. Brain biopsy tissue sample and/or brain biopsy rinse fluid were analyzed by cytomorphology combined with flow cytometry. Eleven patients had both types of sample, four patients had only brain biopsy tissue sample, and three patients had only brain biopsy rinse fluid. Histopathology was used as a reference.
Results
Histopathology characterized ten diffuse large B-cell lymphomas and eight other diseases: three glioblastomas, one necrosis, one Erdheim-Chester disease, one cerebral vasculitis, one stroke and one anaplastic astrocytoma.Cytomorphology and flow cytometry showed lymphoma cells in nine out of the ten lymphomas. Three cytomorphology or flow cytometry negative results were reported for lymphomas in tissue samples due to low cellularity and biopsy sample conditioning. No lymphomatous cells were found by cytomorphology or flow cytometry in the eight other diseases. Rinse fluid results were consistent with histology in all cases studied (sensitivity and specificity, 100%). All lymphomas characterized by flow cytometry were: CD5−, CD20+, Ki67 high and CD10 was expressed in six out of the nine cases as in histopathology. CD44, CD184, BCL2, and BCL6 showed variable expression.The median time to result was 4.5 days (range, 2–10) for histopathology, while 5 h (range, 3–20) were required for both cytomorphology and flow cytometry.
Conclusion
Brain biopsy rinse fluid alleviates problems of tissue sample distribution compared to tissue sample. Its analysis performs diagnosis of B-cell lymphoma in a few hours and, associated with histopathology, allows a multidisciplinary diagnosis. This study shows that cytomorphology combined with flow cytometry on brain biospy rinse fluid is a new, fast, and useful strategy.
Session topic: E-poster
Keyword(s): CNS lymphoma, Diagnosis, Flow cytometry
Type: Publication Only
Background
Central nervous system lymphomas are aggressive tumors requiring a prompt diagnosis for successful treatment. Stereotactic brain biopsy remains the standard procedure, but the time needed for histopathology is usually over 2 days.
Aims
During stereotactic brain biopsy, the trocar is rinsed with saline solution and we evaluated the contribution of cytomorphology and flow cytometry of this rinse fluid usually removed.
Methods
Eighteen patients with suspected localized brain lymphoma underwent stereotactic brain biopsy. Brain biopsy tissue sample and/or brain biopsy rinse fluid were analyzed by cytomorphology combined with flow cytometry. Eleven patients had both types of sample, four patients had only brain biopsy tissue sample, and three patients had only brain biopsy rinse fluid. Histopathology was used as a reference.
Results
Histopathology characterized ten diffuse large B-cell lymphomas and eight other diseases: three glioblastomas, one necrosis, one Erdheim-Chester disease, one cerebral vasculitis, one stroke and one anaplastic astrocytoma.Cytomorphology and flow cytometry showed lymphoma cells in nine out of the ten lymphomas. Three cytomorphology or flow cytometry negative results were reported for lymphomas in tissue samples due to low cellularity and biopsy sample conditioning. No lymphomatous cells were found by cytomorphology or flow cytometry in the eight other diseases. Rinse fluid results were consistent with histology in all cases studied (sensitivity and specificity, 100%). All lymphomas characterized by flow cytometry were: CD5−, CD20+, Ki67 high and CD10 was expressed in six out of the nine cases as in histopathology. CD44, CD184, BCL2, and BCL6 showed variable expression.The median time to result was 4.5 days (range, 2–10) for histopathology, while 5 h (range, 3–20) were required for both cytomorphology and flow cytometry.
Conclusion
Brain biopsy rinse fluid alleviates problems of tissue sample distribution compared to tissue sample. Its analysis performs diagnosis of B-cell lymphoma in a few hours and, associated with histopathology, allows a multidisciplinary diagnosis. This study shows that cytomorphology combined with flow cytometry on brain biospy rinse fluid is a new, fast, and useful strategy.
Session topic: E-poster
Keyword(s): CNS lymphoma, Diagnosis, Flow cytometry
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