CD39 EXPRESSION ON CD4 T INFILTRATING LYMHOCYTES IN THE TUMOR MICROENVIRONMENT OF LYMPHOMAS
(Abstract release date: 05/19/16)
EHA Library. Di Gaetano R. 06/09/16; 134945; PB2045
Dr. Rosa Di Gaetano
Contributions
Contributions
Abstract
Abstract: PB2045
Type: Publication Only
Background
In tumor microenvironment (TME) a variety of mechanisms favor a tumor-protection, one of them is the accumulation of extracellular adenosine (ADO) by up-regulating activity of ectoenzime CD39. The generation of ADO from ATP metabolism is recognized as a mechanism for immunosuppressive function of regulatoty T cell (Treg), a component of the CD4+ T lymphocytes expressing variable functional molecules and involved in tumor escape
Aims
It has been well-documented the increase of Treg in various solid tumor and the higher CD39 expression in some types of human cancer. We have evaluated the CD39 and the CD26 (an adenosine deaminase) on CD4+T infiltrating lymphocytes in lymph node with lymphoma to verify the expression of two markers and whether they could be an attractive features for analysis of TME of a non Hodgkin lymphoma (NHL)
Methods
We analyzed by flow cytometry (FC) immunophenotyping non-neoplastic (10) and B-NHL (48) lymph node samples. and we measured the CD26 and CD39 on CD4+ T lymphocytes in all tissues. Among B-NHL were 14 diffuse large B cell lymphoma (DLBCL) and 3 relapsed
Results
Compared to non-neoplastic samples, CD4+T cells of NHL showed statistically significant difference on CD26 (67% vs 39.5%; p < 0.001) and on CD39 (6% vs 34%; p < 0.001). In NHL, compared to other entities the value of CD26 on DLBCL (39.5% vs 34%) and on the relapsed (39.5% vs 29%) was not significantly different; while CD39 on both groups, DLBCL (34% vs 54%; p <0.005) and relapsed (34% vs 74%; p <0.001), resulted significantly increased.
Conclusion
Although the regulatory activity of immune system can employ a diversity of molecular mechanisms, the presence of enzymatically-competent CD39 on surface of infiltrating CD4+T suggest that the CD39 pathway can be a distinct mechanism utilized by Treg in TME of lymphomas. Furthermore, the tendency to the reduction (heightened in the relapsed) of CD26, connectable to a lower activity of adenosine deaminase, beside the up-regulated expression of CD39, could underlies an increased accumulation of extracellular ADO and its important role in Treg-mediated immune response suppression. Data of DLBCL, resembling the relapsed, tend to differentiate from other entities for a more suppressive functional profile. Together, these simple and fast assays obtained by FC support the finding that ATP-ectonucleotidase-adenosine system may have a role in mediating Treg activity also in lymphomas suggesting that CD39 represents a possible target for cancer immunotherapy.
Session topic: E-poster
Keyword(s): Lymphoma, Microenvironment, T regulatory cells
Type: Publication Only
Background
In tumor microenvironment (TME) a variety of mechanisms favor a tumor-protection, one of them is the accumulation of extracellular adenosine (ADO) by up-regulating activity of ectoenzime CD39. The generation of ADO from ATP metabolism is recognized as a mechanism for immunosuppressive function of regulatoty T cell (Treg), a component of the CD4+ T lymphocytes expressing variable functional molecules and involved in tumor escape
Aims
It has been well-documented the increase of Treg in various solid tumor and the higher CD39 expression in some types of human cancer. We have evaluated the CD39 and the CD26 (an adenosine deaminase) on CD4+T infiltrating lymphocytes in lymph node with lymphoma to verify the expression of two markers and whether they could be an attractive features for analysis of TME of a non Hodgkin lymphoma (NHL)
Methods
We analyzed by flow cytometry (FC) immunophenotyping non-neoplastic (10) and B-NHL (48) lymph node samples. and we measured the CD26 and CD39 on CD4+ T lymphocytes in all tissues. Among B-NHL were 14 diffuse large B cell lymphoma (DLBCL) and 3 relapsed
Results
Compared to non-neoplastic samples, CD4+T cells of NHL showed statistically significant difference on CD26 (67% vs 39.5%; p < 0.001) and on CD39 (6% vs 34%; p < 0.001). In NHL, compared to other entities the value of CD26 on DLBCL (39.5% vs 34%) and on the relapsed (39.5% vs 29%) was not significantly different; while CD39 on both groups, DLBCL (34% vs 54%; p <0.005) and relapsed (34% vs 74%; p <0.001), resulted significantly increased.
Conclusion
Although the regulatory activity of immune system can employ a diversity of molecular mechanisms, the presence of enzymatically-competent CD39 on surface of infiltrating CD4+T suggest that the CD39 pathway can be a distinct mechanism utilized by Treg in TME of lymphomas. Furthermore, the tendency to the reduction (heightened in the relapsed) of CD26, connectable to a lower activity of adenosine deaminase, beside the up-regulated expression of CD39, could underlies an increased accumulation of extracellular ADO and its important role in Treg-mediated immune response suppression. Data of DLBCL, resembling the relapsed, tend to differentiate from other entities for a more suppressive functional profile. Together, these simple and fast assays obtained by FC support the finding that ATP-ectonucleotidase-adenosine system may have a role in mediating Treg activity also in lymphomas suggesting that CD39 represents a possible target for cancer immunotherapy.
Session topic: E-poster
Keyword(s): Lymphoma, Microenvironment, T regulatory cells
Abstract: PB2045
Type: Publication Only
Background
In tumor microenvironment (TME) a variety of mechanisms favor a tumor-protection, one of them is the accumulation of extracellular adenosine (ADO) by up-regulating activity of ectoenzime CD39. The generation of ADO from ATP metabolism is recognized as a mechanism for immunosuppressive function of regulatoty T cell (Treg), a component of the CD4+ T lymphocytes expressing variable functional molecules and involved in tumor escape
Aims
It has been well-documented the increase of Treg in various solid tumor and the higher CD39 expression in some types of human cancer. We have evaluated the CD39 and the CD26 (an adenosine deaminase) on CD4+T infiltrating lymphocytes in lymph node with lymphoma to verify the expression of two markers and whether they could be an attractive features for analysis of TME of a non Hodgkin lymphoma (NHL)
Methods
We analyzed by flow cytometry (FC) immunophenotyping non-neoplastic (10) and B-NHL (48) lymph node samples. and we measured the CD26 and CD39 on CD4+ T lymphocytes in all tissues. Among B-NHL were 14 diffuse large B cell lymphoma (DLBCL) and 3 relapsed
Results
Compared to non-neoplastic samples, CD4+T cells of NHL showed statistically significant difference on CD26 (67% vs 39.5%; p < 0.001) and on CD39 (6% vs 34%; p < 0.001). In NHL, compared to other entities the value of CD26 on DLBCL (39.5% vs 34%) and on the relapsed (39.5% vs 29%) was not significantly different; while CD39 on both groups, DLBCL (34% vs 54%; p <0.005) and relapsed (34% vs 74%; p <0.001), resulted significantly increased.
Conclusion
Although the regulatory activity of immune system can employ a diversity of molecular mechanisms, the presence of enzymatically-competent CD39 on surface of infiltrating CD4+T suggest that the CD39 pathway can be a distinct mechanism utilized by Treg in TME of lymphomas. Furthermore, the tendency to the reduction (heightened in the relapsed) of CD26, connectable to a lower activity of adenosine deaminase, beside the up-regulated expression of CD39, could underlies an increased accumulation of extracellular ADO and its important role in Treg-mediated immune response suppression. Data of DLBCL, resembling the relapsed, tend to differentiate from other entities for a more suppressive functional profile. Together, these simple and fast assays obtained by FC support the finding that ATP-ectonucleotidase-adenosine system may have a role in mediating Treg activity also in lymphomas suggesting that CD39 represents a possible target for cancer immunotherapy.
Session topic: E-poster
Keyword(s): Lymphoma, Microenvironment, T regulatory cells
Type: Publication Only
Background
In tumor microenvironment (TME) a variety of mechanisms favor a tumor-protection, one of them is the accumulation of extracellular adenosine (ADO) by up-regulating activity of ectoenzime CD39. The generation of ADO from ATP metabolism is recognized as a mechanism for immunosuppressive function of regulatoty T cell (Treg), a component of the CD4+ T lymphocytes expressing variable functional molecules and involved in tumor escape
Aims
It has been well-documented the increase of Treg in various solid tumor and the higher CD39 expression in some types of human cancer. We have evaluated the CD39 and the CD26 (an adenosine deaminase) on CD4+T infiltrating lymphocytes in lymph node with lymphoma to verify the expression of two markers and whether they could be an attractive features for analysis of TME of a non Hodgkin lymphoma (NHL)
Methods
We analyzed by flow cytometry (FC) immunophenotyping non-neoplastic (10) and B-NHL (48) lymph node samples. and we measured the CD26 and CD39 on CD4+ T lymphocytes in all tissues. Among B-NHL were 14 diffuse large B cell lymphoma (DLBCL) and 3 relapsed
Results
Compared to non-neoplastic samples, CD4+T cells of NHL showed statistically significant difference on CD26 (67% vs 39.5%; p < 0.001) and on CD39 (6% vs 34%; p < 0.001). In NHL, compared to other entities the value of CD26 on DLBCL (39.5% vs 34%) and on the relapsed (39.5% vs 29%) was not significantly different; while CD39 on both groups, DLBCL (34% vs 54%; p <0.005) and relapsed (34% vs 74%; p <0.001), resulted significantly increased.
Conclusion
Although the regulatory activity of immune system can employ a diversity of molecular mechanisms, the presence of enzymatically-competent CD39 on surface of infiltrating CD4+T suggest that the CD39 pathway can be a distinct mechanism utilized by Treg in TME of lymphomas. Furthermore, the tendency to the reduction (heightened in the relapsed) of CD26, connectable to a lower activity of adenosine deaminase, beside the up-regulated expression of CD39, could underlies an increased accumulation of extracellular ADO and its important role in Treg-mediated immune response suppression. Data of DLBCL, resembling the relapsed, tend to differentiate from other entities for a more suppressive functional profile. Together, these simple and fast assays obtained by FC support the finding that ATP-ectonucleotidase-adenosine system may have a role in mediating Treg activity also in lymphomas suggesting that CD39 represents a possible target for cancer immunotherapy.
Session topic: E-poster
Keyword(s): Lymphoma, Microenvironment, T regulatory cells
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