THE EFFECT OF MIR-146A OVEREXPRESSION ON THE PROLIFERATION AND APOPTOSIS OF MULTIPLE MYELOMA CELL LINE
(Abstract release date: 05/19/16)
EHA Library. Kazemzadeh S. 06/09/16; 134847; PB1947
Ms. Shima Kazemzadeh
Contributions
Contributions
Abstract
Abstract: PB1947
Type: Publication Only
Background
After lymphoma, multiple myeloma (MM) is the second most common hematological malignancy worldwide contributing 1% of all cancers and nearly 2% of cancer mortalities. Deregulation of microRNAs has been implicated in the pathogenesis of multiple myeloma. miR-146a is one of the most important microRNAs in tumor initiation and progression which has a dual effect and act as a tumor promoter or a tumor suppressor.
Aims
The present study aimed to evaluate the functional effect of miR-146a in multiple myeloma cells.
Methods
The expression of miR-146a was examined in myeloma cell line, L363 by qRT-PCR. The effect of overexpression of miR-146a on proliferation and apoptosis of myeloma cells was further evaluated by lentiviral-based delivery method.
Results
According to our results, the expression of miR-146a significantly increased in cells transducted with transfer vector compered to non-transducted cells (P-value=0.001). Transfection of miR-146a resulted in significant decrease in tumor cell proliferation (P-value=0.001) and significant increase in apoptosis of multiple myeloma cells (P-value=0.004).
Conclusion
According to previous studies, miR-146a has a dual effect in cancers. Our result suggested that miR-146a acts as a tumor suppressor in MM. It seems that miR-146a regulates an miR-146-NF-κB negative feedback regulation loop in myeloma cells by inhibiting the expression of IRAK1 and TRAF6 and consequently prevents tumor cell proliferation and enhanced apoptosis.
Session topic: E-poster
Keyword(s): Apoptosis, Multiple myeloma, Proliferation, Transfection
Type: Publication Only
Background
After lymphoma, multiple myeloma (MM) is the second most common hematological malignancy worldwide contributing 1% of all cancers and nearly 2% of cancer mortalities. Deregulation of microRNAs has been implicated in the pathogenesis of multiple myeloma. miR-146a is one of the most important microRNAs in tumor initiation and progression which has a dual effect and act as a tumor promoter or a tumor suppressor.
Aims
The present study aimed to evaluate the functional effect of miR-146a in multiple myeloma cells.
Methods
The expression of miR-146a was examined in myeloma cell line, L363 by qRT-PCR. The effect of overexpression of miR-146a on proliferation and apoptosis of myeloma cells was further evaluated by lentiviral-based delivery method.
Results
According to our results, the expression of miR-146a significantly increased in cells transducted with transfer vector compered to non-transducted cells (P-value=0.001). Transfection of miR-146a resulted in significant decrease in tumor cell proliferation (P-value=0.001) and significant increase in apoptosis of multiple myeloma cells (P-value=0.004).
Conclusion
According to previous studies, miR-146a has a dual effect in cancers. Our result suggested that miR-146a acts as a tumor suppressor in MM. It seems that miR-146a regulates an miR-146-NF-κB negative feedback regulation loop in myeloma cells by inhibiting the expression of IRAK1 and TRAF6 and consequently prevents tumor cell proliferation and enhanced apoptosis.
Session topic: E-poster
Keyword(s): Apoptosis, Multiple myeloma, Proliferation, Transfection
Abstract: PB1947
Type: Publication Only
Background
After lymphoma, multiple myeloma (MM) is the second most common hematological malignancy worldwide contributing 1% of all cancers and nearly 2% of cancer mortalities. Deregulation of microRNAs has been implicated in the pathogenesis of multiple myeloma. miR-146a is one of the most important microRNAs in tumor initiation and progression which has a dual effect and act as a tumor promoter or a tumor suppressor.
Aims
The present study aimed to evaluate the functional effect of miR-146a in multiple myeloma cells.
Methods
The expression of miR-146a was examined in myeloma cell line, L363 by qRT-PCR. The effect of overexpression of miR-146a on proliferation and apoptosis of myeloma cells was further evaluated by lentiviral-based delivery method.
Results
According to our results, the expression of miR-146a significantly increased in cells transducted with transfer vector compered to non-transducted cells (P-value=0.001). Transfection of miR-146a resulted in significant decrease in tumor cell proliferation (P-value=0.001) and significant increase in apoptosis of multiple myeloma cells (P-value=0.004).
Conclusion
According to previous studies, miR-146a has a dual effect in cancers. Our result suggested that miR-146a acts as a tumor suppressor in MM. It seems that miR-146a regulates an miR-146-NF-κB negative feedback regulation loop in myeloma cells by inhibiting the expression of IRAK1 and TRAF6 and consequently prevents tumor cell proliferation and enhanced apoptosis.
Session topic: E-poster
Keyword(s): Apoptosis, Multiple myeloma, Proliferation, Transfection
Type: Publication Only
Background
After lymphoma, multiple myeloma (MM) is the second most common hematological malignancy worldwide contributing 1% of all cancers and nearly 2% of cancer mortalities. Deregulation of microRNAs has been implicated in the pathogenesis of multiple myeloma. miR-146a is one of the most important microRNAs in tumor initiation and progression which has a dual effect and act as a tumor promoter or a tumor suppressor.
Aims
The present study aimed to evaluate the functional effect of miR-146a in multiple myeloma cells.
Methods
The expression of miR-146a was examined in myeloma cell line, L363 by qRT-PCR. The effect of overexpression of miR-146a on proliferation and apoptosis of myeloma cells was further evaluated by lentiviral-based delivery method.
Results
According to our results, the expression of miR-146a significantly increased in cells transducted with transfer vector compered to non-transducted cells (P-value=0.001). Transfection of miR-146a resulted in significant decrease in tumor cell proliferation (P-value=0.001) and significant increase in apoptosis of multiple myeloma cells (P-value=0.004).
Conclusion
According to previous studies, miR-146a has a dual effect in cancers. Our result suggested that miR-146a acts as a tumor suppressor in MM. It seems that miR-146a regulates an miR-146-NF-κB negative feedback regulation loop in myeloma cells by inhibiting the expression of IRAK1 and TRAF6 and consequently prevents tumor cell proliferation and enhanced apoptosis.
Session topic: E-poster
Keyword(s): Apoptosis, Multiple myeloma, Proliferation, Transfection
{{ help_message }}
{{filter}}