IL-1R AND IL-1RA IN THE DEVELOPMENT OF MULTIPLE MYELOMA
(Abstract release date: 05/19/16)
EHA Library. Pavlova A. 06/09/16; 134842; PB1942
Ms. Anastasia Pavlova
Contributions
Contributions
Abstract
Abstract: PB1942
Type: Publication Only
Background
Various cytokines are involved in the pathogenesis of Multiple myeloma (MM). They play a significant role in the development of tumor clone. It is known that single nucleotide polymorphisms (SNP) in the gene regulatory regions can influence on cytokines production which can effect on the development of the disease.
Aims
The aim of this study was to identify SNP genes of IL-1R (pst11970C/T) and IL-1RA (mspa111100T/C) associated with the development of MM among the residents of the North-West region of Russia as well as determining the severity of bone tissue damage.
Methods
We analyzed 50 MM patients which were divided into two groups depending on the detected changes in the bones (median age: 69.7 ± 8.6): 1st group (22 patients) - with severe osteolytic bone lesions (III stage of the Durie-Salmon staging system); 2nd group (28 patients) – with manifestations of osteoporosis and isolated pockets of lysis (II stage of of the Durie-Salmon staging system). The diagnosis of symptomatic MM was verified based on the criteria of the International Working Group on myeloma. The most frequent type of myeloma that was observed in patients was G type (80%). The frequencies of myeloma A, as well as disease of light chains were about 20%. The control group consisted of 50 healthy unrelated Caucasoid blood donors (median age: 51.2±6.9). All analyzed people were from St.-Petersburg, Russia. Genomic DNA was extracted from the peripheral blood and gene genotyping was performed by use of PCR-SSP (Cytokine genotyping Kit, Invitrogen). Allele frequencies and expected Hardy Weinberg equilibrium (HWE) for each SNP were determined. P values less than 0.05 were considered statistically significant.
Results
Based on the SNP analyses we found that in general cohort of patients with MM some genotype frequencies differed from the control group. For instance, genotypes of IL-1RTT and IL-1RATT were varied in control group and group of patients 0.33 vs. 0.16 and 0.28 vs. 0.46 respectively (р≥0.05). However, genotype IL-1RTT in MM patients with severe bone lesions (1st gr.) occurs more often than in group of patients with symptoms of osteoporosis (2nd gr.): 0.33 vs. 0.06 respectively (p≤0.05). Although, genotype IL-1RCT in the 2nd gr. was registered frequently compare to 1st gr. and control group- 0.50 vs. 0.33 and 0.33 respectively (р≥0.05). However, IL-1RATT genotype frequency was less common in healthy people (0.28) compare to patients with MM (0.45 in 1st gr., 0.47 in 2nd gr.; p≤0.05). Finally, IL-1RACT was higher in control group (0.50) compare to patients with severe osteolytic bone lesions (0.33) and patients with symptoms of osteoporosis (0.40), р≥0.05.
Conclusion
Thus, our results allow to describe some genotypes as markers associated with the development of MM (IL-1RATT). In addition, it can be assumed that the genotype IL-1RTT associated with the development of severe osteolytic bone lesions in multiple myeloma in turn genotype IL-1RCT as an immunogenetic marker of a lighter form of bone disease manifested with osteoporosis and isolated pockets of lysis.
Session topic: E-poster
Keyword(s): Cytokine, Multiple myeloma
Type: Publication Only
Background
Various cytokines are involved in the pathogenesis of Multiple myeloma (MM). They play a significant role in the development of tumor clone. It is known that single nucleotide polymorphisms (SNP) in the gene regulatory regions can influence on cytokines production which can effect on the development of the disease.
Aims
The aim of this study was to identify SNP genes of IL-1R (pst11970C/T) and IL-1RA (mspa111100T/C) associated with the development of MM among the residents of the North-West region of Russia as well as determining the severity of bone tissue damage.
Methods
We analyzed 50 MM patients which were divided into two groups depending on the detected changes in the bones (median age: 69.7 ± 8.6): 1st group (22 patients) - with severe osteolytic bone lesions (III stage of the Durie-Salmon staging system); 2nd group (28 patients) – with manifestations of osteoporosis and isolated pockets of lysis (II stage of of the Durie-Salmon staging system). The diagnosis of symptomatic MM was verified based on the criteria of the International Working Group on myeloma. The most frequent type of myeloma that was observed in patients was G type (80%). The frequencies of myeloma A, as well as disease of light chains were about 20%. The control group consisted of 50 healthy unrelated Caucasoid blood donors (median age: 51.2±6.9). All analyzed people were from St.-Petersburg, Russia. Genomic DNA was extracted from the peripheral blood and gene genotyping was performed by use of PCR-SSP (Cytokine genotyping Kit, Invitrogen). Allele frequencies and expected Hardy Weinberg equilibrium (HWE) for each SNP were determined. P values less than 0.05 were considered statistically significant.
Results
Based on the SNP analyses we found that in general cohort of patients with MM some genotype frequencies differed from the control group. For instance, genotypes of IL-1RTT and IL-1RATT were varied in control group and group of patients 0.33 vs. 0.16 and 0.28 vs. 0.46 respectively (р≥0.05). However, genotype IL-1RTT in MM patients with severe bone lesions (1st gr.) occurs more often than in group of patients with symptoms of osteoporosis (2nd gr.): 0.33 vs. 0.06 respectively (p≤0.05). Although, genotype IL-1RCT in the 2nd gr. was registered frequently compare to 1st gr. and control group- 0.50 vs. 0.33 and 0.33 respectively (р≥0.05). However, IL-1RATT genotype frequency was less common in healthy people (0.28) compare to patients with MM (0.45 in 1st gr., 0.47 in 2nd gr.; p≤0.05). Finally, IL-1RACT was higher in control group (0.50) compare to patients with severe osteolytic bone lesions (0.33) and patients with symptoms of osteoporosis (0.40), р≥0.05.
Conclusion
Thus, our results allow to describe some genotypes as markers associated with the development of MM (IL-1RATT). In addition, it can be assumed that the genotype IL-1RTT associated with the development of severe osteolytic bone lesions in multiple myeloma in turn genotype IL-1RCT as an immunogenetic marker of a lighter form of bone disease manifested with osteoporosis and isolated pockets of lysis.
Session topic: E-poster
Keyword(s): Cytokine, Multiple myeloma
Abstract: PB1942
Type: Publication Only
Background
Various cytokines are involved in the pathogenesis of Multiple myeloma (MM). They play a significant role in the development of tumor clone. It is known that single nucleotide polymorphisms (SNP) in the gene regulatory regions can influence on cytokines production which can effect on the development of the disease.
Aims
The aim of this study was to identify SNP genes of IL-1R (pst11970C/T) and IL-1RA (mspa111100T/C) associated with the development of MM among the residents of the North-West region of Russia as well as determining the severity of bone tissue damage.
Methods
We analyzed 50 MM patients which were divided into two groups depending on the detected changes in the bones (median age: 69.7 ± 8.6): 1st group (22 patients) - with severe osteolytic bone lesions (III stage of the Durie-Salmon staging system); 2nd group (28 patients) – with manifestations of osteoporosis and isolated pockets of lysis (II stage of of the Durie-Salmon staging system). The diagnosis of symptomatic MM was verified based on the criteria of the International Working Group on myeloma. The most frequent type of myeloma that was observed in patients was G type (80%). The frequencies of myeloma A, as well as disease of light chains were about 20%. The control group consisted of 50 healthy unrelated Caucasoid blood donors (median age: 51.2±6.9). All analyzed people were from St.-Petersburg, Russia. Genomic DNA was extracted from the peripheral blood and gene genotyping was performed by use of PCR-SSP (Cytokine genotyping Kit, Invitrogen). Allele frequencies and expected Hardy Weinberg equilibrium (HWE) for each SNP were determined. P values less than 0.05 were considered statistically significant.
Results
Based on the SNP analyses we found that in general cohort of patients with MM some genotype frequencies differed from the control group. For instance, genotypes of IL-1RTT and IL-1RATT were varied in control group and group of patients 0.33 vs. 0.16 and 0.28 vs. 0.46 respectively (р≥0.05). However, genotype IL-1RTT in MM patients with severe bone lesions (1st gr.) occurs more often than in group of patients with symptoms of osteoporosis (2nd gr.): 0.33 vs. 0.06 respectively (p≤0.05). Although, genotype IL-1RCT in the 2nd gr. was registered frequently compare to 1st gr. and control group- 0.50 vs. 0.33 and 0.33 respectively (р≥0.05). However, IL-1RATT genotype frequency was less common in healthy people (0.28) compare to patients with MM (0.45 in 1st gr., 0.47 in 2nd gr.; p≤0.05). Finally, IL-1RACT was higher in control group (0.50) compare to patients with severe osteolytic bone lesions (0.33) and patients with symptoms of osteoporosis (0.40), р≥0.05.
Conclusion
Thus, our results allow to describe some genotypes as markers associated with the development of MM (IL-1RATT). In addition, it can be assumed that the genotype IL-1RTT associated with the development of severe osteolytic bone lesions in multiple myeloma in turn genotype IL-1RCT as an immunogenetic marker of a lighter form of bone disease manifested with osteoporosis and isolated pockets of lysis.
Session topic: E-poster
Keyword(s): Cytokine, Multiple myeloma
Type: Publication Only
Background
Various cytokines are involved in the pathogenesis of Multiple myeloma (MM). They play a significant role in the development of tumor clone. It is known that single nucleotide polymorphisms (SNP) in the gene regulatory regions can influence on cytokines production which can effect on the development of the disease.
Aims
The aim of this study was to identify SNP genes of IL-1R (pst11970C/T) and IL-1RA (mspa111100T/C) associated with the development of MM among the residents of the North-West region of Russia as well as determining the severity of bone tissue damage.
Methods
We analyzed 50 MM patients which were divided into two groups depending on the detected changes in the bones (median age: 69.7 ± 8.6): 1st group (22 patients) - with severe osteolytic bone lesions (III stage of the Durie-Salmon staging system); 2nd group (28 patients) – with manifestations of osteoporosis and isolated pockets of lysis (II stage of of the Durie-Salmon staging system). The diagnosis of symptomatic MM was verified based on the criteria of the International Working Group on myeloma. The most frequent type of myeloma that was observed in patients was G type (80%). The frequencies of myeloma A, as well as disease of light chains were about 20%. The control group consisted of 50 healthy unrelated Caucasoid blood donors (median age: 51.2±6.9). All analyzed people were from St.-Petersburg, Russia. Genomic DNA was extracted from the peripheral blood and gene genotyping was performed by use of PCR-SSP (Cytokine genotyping Kit, Invitrogen). Allele frequencies and expected Hardy Weinberg equilibrium (HWE) for each SNP were determined. P values less than 0.05 were considered statistically significant.
Results
Based on the SNP analyses we found that in general cohort of patients with MM some genotype frequencies differed from the control group. For instance, genotypes of IL-1RTT and IL-1RATT were varied in control group and group of patients 0.33 vs. 0.16 and 0.28 vs. 0.46 respectively (р≥0.05). However, genotype IL-1RTT in MM patients with severe bone lesions (1st gr.) occurs more often than in group of patients with symptoms of osteoporosis (2nd gr.): 0.33 vs. 0.06 respectively (p≤0.05). Although, genotype IL-1RCT in the 2nd gr. was registered frequently compare to 1st gr. and control group- 0.50 vs. 0.33 and 0.33 respectively (р≥0.05). However, IL-1RATT genotype frequency was less common in healthy people (0.28) compare to patients with MM (0.45 in 1st gr., 0.47 in 2nd gr.; p≤0.05). Finally, IL-1RACT was higher in control group (0.50) compare to patients with severe osteolytic bone lesions (0.33) and patients with symptoms of osteoporosis (0.40), р≥0.05.
Conclusion
Thus, our results allow to describe some genotypes as markers associated with the development of MM (IL-1RATT). In addition, it can be assumed that the genotype IL-1RTT associated with the development of severe osteolytic bone lesions in multiple myeloma in turn genotype IL-1RCT as an immunogenetic marker of a lighter form of bone disease manifested with osteoporosis and isolated pockets of lysis.
Session topic: E-poster
Keyword(s): Cytokine, Multiple myeloma
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