INVESTIGATION OF RELATIONSHIP BETWEEN NEUTROPENIA AND SERUM CHITOTRIOSIDASE AND NEUTROPHIL GELATINASE RELATED LIPOCALIN ENZYME ACTIVITY IN CHILDREN UNDERGOING CHEMOTHERAPY
(Abstract release date: 05/19/16)
EHA Library. Gursel O. 06/09/16; 134787; PB1887
Assoc. Prof. Orhan Gursel
Contributions
Contributions
Abstract
Abstract: PB1887
Type: Publication Only
Background
Human chitotriosidase (ChT) is a member of the chitinase family and it is synthesized and secreted by activated macrophages and neutrophilic granulocyte progenitors. Neutrophil gelatinase related lipocalin (NGAL) is situated in secondary granules of granulocytes and is specific to neutrophils.
Aims
The aim of this study was to investigate the relationship between neutropenia and serum ChT and NGAL levels in patients with hematologic malignancy and hospitalized because of febrile neutropenia.
Methods
This study included 27 children between 1-15 years old, who have hematologic malignancies and whose chemotherapies were still ongoing and were known not having a disease that leads to macrophage activation. Complete blood count (CBC),serum levels of c-reactive protein (CRP), lactate dehydrogenase (LDH), ChT and NGAL were determined before the initiation of chemotherapy while the patients were not neutropenic. The same tests were studied also in the severe neutropenic period and after the recovery of neutropenia. Serum ChT and NGAL levels of the patients during these periods were compared. Whether there is a correlation between the number of neutrophils and these enzyme levels were also investigated.
Results
Total granulocyte count (TGC), CRP, LDH, NGAL and ChT levels were statistically significant different between the periods of severe neutropenia, improving neutropenia and recovered neutropenia (p:0,0001). There was a statistically significant positive correlation between ChT and TGC levels during the period of severe neutropenia (p:0.034, Pearson’s r: 0,418). There was not a statistically significant relationship between NGAL and TGC levels during the period of severe neutropenia.
Conclusion
The results of our study exhibiting a statistically significant difference, between serum ChT and NGAL levels, during the periods of severe neutropenia, improving neutropenia and recovered neutropenia, supports the data suggesting that these enzymes are secreted by neutrophils. Especially, statistically significant positive correlation between ChT and TGC levels suggests that levels of this enzyme can be used as a marker to determine the period of recovery from neutropenia. Further studies are needed to investigate the relationship between febrile neutropenia prognosis and NGAL and Cht levels.
Session topic: E-poster
Keyword(s): Chemotherapy, Children, Neutropenia
Type: Publication Only
Background
Human chitotriosidase (ChT) is a member of the chitinase family and it is synthesized and secreted by activated macrophages and neutrophilic granulocyte progenitors. Neutrophil gelatinase related lipocalin (NGAL) is situated in secondary granules of granulocytes and is specific to neutrophils.
Aims
The aim of this study was to investigate the relationship between neutropenia and serum ChT and NGAL levels in patients with hematologic malignancy and hospitalized because of febrile neutropenia.
Methods
This study included 27 children between 1-15 years old, who have hematologic malignancies and whose chemotherapies were still ongoing and were known not having a disease that leads to macrophage activation. Complete blood count (CBC),serum levels of c-reactive protein (CRP), lactate dehydrogenase (LDH), ChT and NGAL were determined before the initiation of chemotherapy while the patients were not neutropenic. The same tests were studied also in the severe neutropenic period and after the recovery of neutropenia. Serum ChT and NGAL levels of the patients during these periods were compared. Whether there is a correlation between the number of neutrophils and these enzyme levels were also investigated.
Results
Total granulocyte count (TGC), CRP, LDH, NGAL and ChT levels were statistically significant different between the periods of severe neutropenia, improving neutropenia and recovered neutropenia (p:0,0001). There was a statistically significant positive correlation between ChT and TGC levels during the period of severe neutropenia (p:0.034, Pearson’s r: 0,418). There was not a statistically significant relationship between NGAL and TGC levels during the period of severe neutropenia.
Conclusion
The results of our study exhibiting a statistically significant difference, between serum ChT and NGAL levels, during the periods of severe neutropenia, improving neutropenia and recovered neutropenia, supports the data suggesting that these enzymes are secreted by neutrophils. Especially, statistically significant positive correlation between ChT and TGC levels suggests that levels of this enzyme can be used as a marker to determine the period of recovery from neutropenia. Further studies are needed to investigate the relationship between febrile neutropenia prognosis and NGAL and Cht levels.
Session topic: E-poster
Keyword(s): Chemotherapy, Children, Neutropenia
Abstract: PB1887
Type: Publication Only
Background
Human chitotriosidase (ChT) is a member of the chitinase family and it is synthesized and secreted by activated macrophages and neutrophilic granulocyte progenitors. Neutrophil gelatinase related lipocalin (NGAL) is situated in secondary granules of granulocytes and is specific to neutrophils.
Aims
The aim of this study was to investigate the relationship between neutropenia and serum ChT and NGAL levels in patients with hematologic malignancy and hospitalized because of febrile neutropenia.
Methods
This study included 27 children between 1-15 years old, who have hematologic malignancies and whose chemotherapies were still ongoing and were known not having a disease that leads to macrophage activation. Complete blood count (CBC),serum levels of c-reactive protein (CRP), lactate dehydrogenase (LDH), ChT and NGAL were determined before the initiation of chemotherapy while the patients were not neutropenic. The same tests were studied also in the severe neutropenic period and after the recovery of neutropenia. Serum ChT and NGAL levels of the patients during these periods were compared. Whether there is a correlation between the number of neutrophils and these enzyme levels were also investigated.
Results
Total granulocyte count (TGC), CRP, LDH, NGAL and ChT levels were statistically significant different between the periods of severe neutropenia, improving neutropenia and recovered neutropenia (p:0,0001). There was a statistically significant positive correlation between ChT and TGC levels during the period of severe neutropenia (p:0.034, Pearson’s r: 0,418). There was not a statistically significant relationship between NGAL and TGC levels during the period of severe neutropenia.
Conclusion
The results of our study exhibiting a statistically significant difference, between serum ChT and NGAL levels, during the periods of severe neutropenia, improving neutropenia and recovered neutropenia, supports the data suggesting that these enzymes are secreted by neutrophils. Especially, statistically significant positive correlation between ChT and TGC levels suggests that levels of this enzyme can be used as a marker to determine the period of recovery from neutropenia. Further studies are needed to investigate the relationship between febrile neutropenia prognosis and NGAL and Cht levels.
Session topic: E-poster
Keyword(s): Chemotherapy, Children, Neutropenia
Type: Publication Only
Background
Human chitotriosidase (ChT) is a member of the chitinase family and it is synthesized and secreted by activated macrophages and neutrophilic granulocyte progenitors. Neutrophil gelatinase related lipocalin (NGAL) is situated in secondary granules of granulocytes and is specific to neutrophils.
Aims
The aim of this study was to investigate the relationship between neutropenia and serum ChT and NGAL levels in patients with hematologic malignancy and hospitalized because of febrile neutropenia.
Methods
This study included 27 children between 1-15 years old, who have hematologic malignancies and whose chemotherapies were still ongoing and were known not having a disease that leads to macrophage activation. Complete blood count (CBC),serum levels of c-reactive protein (CRP), lactate dehydrogenase (LDH), ChT and NGAL were determined before the initiation of chemotherapy while the patients were not neutropenic. The same tests were studied also in the severe neutropenic period and after the recovery of neutropenia. Serum ChT and NGAL levels of the patients during these periods were compared. Whether there is a correlation between the number of neutrophils and these enzyme levels were also investigated.
Results
Total granulocyte count (TGC), CRP, LDH, NGAL and ChT levels were statistically significant different between the periods of severe neutropenia, improving neutropenia and recovered neutropenia (p:0,0001). There was a statistically significant positive correlation between ChT and TGC levels during the period of severe neutropenia (p:0.034, Pearson’s r: 0,418). There was not a statistically significant relationship between NGAL and TGC levels during the period of severe neutropenia.
Conclusion
The results of our study exhibiting a statistically significant difference, between serum ChT and NGAL levels, during the periods of severe neutropenia, improving neutropenia and recovered neutropenia, supports the data suggesting that these enzymes are secreted by neutrophils. Especially, statistically significant positive correlation between ChT and TGC levels suggests that levels of this enzyme can be used as a marker to determine the period of recovery from neutropenia. Further studies are needed to investigate the relationship between febrile neutropenia prognosis and NGAL and Cht levels.
Session topic: E-poster
Keyword(s): Chemotherapy, Children, Neutropenia
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