THE RELATIONSHIP BETWEEN BONE MARROW STROMAL FIBROBLASTS, MYELOGRAM PERFORMANCE AND EFFICIENCY OF CHEMOTHERAPY IN CHILDREN WITH ACUTE LEUKEMIA
(Abstract release date: 05/19/16)
EHA Library. Bruslova K. 06/09/16; 134751; PB1851
Prof. Katherine Bruslova
Contributions
Contributions
Abstract
Abstract: PB1851
Type: Publication Only
Background
Tumor microenvironment plays an important role in cancer initiation and progression. The cancer associated fibroblasts impact on resistance development to chemotherapeutic agents in the treatment of acute leukemia (AL) discussed.
Aims
The aim of the study was to determine the relation between colony-forming efficiency of bone marrow stromal fibroblasts, myelogram parameters and effectiveness of chemotherapy (CT) in children with lympho- and myelo- variants of acute leukemia (ALL, AML).
Methods
The study involved 38 children: ALL - 24, AML - 14. Age of children ranged from 6 to 18 years. Treatment was performed by standard protocols. Investigation of patients executed in complete remission phase after full chemotherapy course of primary diagnosis leukemia. Retrospective surveillance was conducted for 15 years. The effectiveness of chemotherapy evaluated by presence/absence of AL relapse. Among patients with ALL the relapse stated in 8 persons, among AML - in 7 children. Efficiency of bone marrow stromal fibroblast colony-forming units (E -CFUf) in vitro, myelogram performance were studied. Spearman correlation coefficient, U- test were used.
Results
E-CFUf not depend on the variant of acute leukemia (Uemp. = 61.5). In patients with AL we found the inverse correlation between the E-CFUf index and the percentage of erythroblasts (Rs = - 0,52) and monocytes (Rs = - 0,45) in the bone marrow. The direct connection between the number of monocytes and erythroblasts in myelogram defined (Rs = + 0,59), probably because of their joint operation in the development of erythroid elements of hematopoiesis. The inverse correlation( Rs = -0,44) between the percentage of monocytes in patients myelogram and AL relapse occurence was found.
Conclusion
The inverse correlation between stromal fibroblasts and erythroblasts as monocytes in the bone marrow of AL children indicates the regulatory role of stromal microenvironment elements in the functioning of hematopoiesis in the development of malignancy. Discovered correlation between relapse development and the number of monocytes in the AL patients bone marrow after end of protocol treatment opens up further possibilities to determine the mechanisms of chemoresistance formation at AL and review the ways to prognose the effectiveness of treatment of such patients.
Session topic: E-poster
Keyword(s): Chemoresistance, Leukemia, Microenvironment, Prognosis
Type: Publication Only
Background
Tumor microenvironment plays an important role in cancer initiation and progression. The cancer associated fibroblasts impact on resistance development to chemotherapeutic agents in the treatment of acute leukemia (AL) discussed.
Aims
The aim of the study was to determine the relation between colony-forming efficiency of bone marrow stromal fibroblasts, myelogram parameters and effectiveness of chemotherapy (CT) in children with lympho- and myelo- variants of acute leukemia (ALL, AML).
Methods
The study involved 38 children: ALL - 24, AML - 14. Age of children ranged from 6 to 18 years. Treatment was performed by standard protocols. Investigation of patients executed in complete remission phase after full chemotherapy course of primary diagnosis leukemia. Retrospective surveillance was conducted for 15 years. The effectiveness of chemotherapy evaluated by presence/absence of AL relapse. Among patients with ALL the relapse stated in 8 persons, among AML - in 7 children. Efficiency of bone marrow stromal fibroblast colony-forming units (E -CFUf) in vitro, myelogram performance were studied. Spearman correlation coefficient, U- test were used.
Results
E-CFUf not depend on the variant of acute leukemia (Uemp. = 61.5). In patients with AL we found the inverse correlation between the E-CFUf index and the percentage of erythroblasts (Rs = - 0,52) and monocytes (Rs = - 0,45) in the bone marrow. The direct connection between the number of monocytes and erythroblasts in myelogram defined (Rs = + 0,59), probably because of their joint operation in the development of erythroid elements of hematopoiesis. The inverse correlation( Rs = -0,44) between the percentage of monocytes in patients myelogram and AL relapse occurence was found.
Conclusion
The inverse correlation between stromal fibroblasts and erythroblasts as monocytes in the bone marrow of AL children indicates the regulatory role of stromal microenvironment elements in the functioning of hematopoiesis in the development of malignancy. Discovered correlation between relapse development and the number of monocytes in the AL patients bone marrow after end of protocol treatment opens up further possibilities to determine the mechanisms of chemoresistance formation at AL and review the ways to prognose the effectiveness of treatment of such patients.
Session topic: E-poster
Keyword(s): Chemoresistance, Leukemia, Microenvironment, Prognosis
Abstract: PB1851
Type: Publication Only
Background
Tumor microenvironment plays an important role in cancer initiation and progression. The cancer associated fibroblasts impact on resistance development to chemotherapeutic agents in the treatment of acute leukemia (AL) discussed.
Aims
The aim of the study was to determine the relation between colony-forming efficiency of bone marrow stromal fibroblasts, myelogram parameters and effectiveness of chemotherapy (CT) in children with lympho- and myelo- variants of acute leukemia (ALL, AML).
Methods
The study involved 38 children: ALL - 24, AML - 14. Age of children ranged from 6 to 18 years. Treatment was performed by standard protocols. Investigation of patients executed in complete remission phase after full chemotherapy course of primary diagnosis leukemia. Retrospective surveillance was conducted for 15 years. The effectiveness of chemotherapy evaluated by presence/absence of AL relapse. Among patients with ALL the relapse stated in 8 persons, among AML - in 7 children. Efficiency of bone marrow stromal fibroblast colony-forming units (E -CFUf) in vitro, myelogram performance were studied. Spearman correlation coefficient, U- test were used.
Results
E-CFUf not depend on the variant of acute leukemia (Uemp. = 61.5). In patients with AL we found the inverse correlation between the E-CFUf index and the percentage of erythroblasts (Rs = - 0,52) and monocytes (Rs = - 0,45) in the bone marrow. The direct connection between the number of monocytes and erythroblasts in myelogram defined (Rs = + 0,59), probably because of their joint operation in the development of erythroid elements of hematopoiesis. The inverse correlation( Rs = -0,44) between the percentage of monocytes in patients myelogram and AL relapse occurence was found.
Conclusion
The inverse correlation between stromal fibroblasts and erythroblasts as monocytes in the bone marrow of AL children indicates the regulatory role of stromal microenvironment elements in the functioning of hematopoiesis in the development of malignancy. Discovered correlation between relapse development and the number of monocytes in the AL patients bone marrow after end of protocol treatment opens up further possibilities to determine the mechanisms of chemoresistance formation at AL and review the ways to prognose the effectiveness of treatment of such patients.
Session topic: E-poster
Keyword(s): Chemoresistance, Leukemia, Microenvironment, Prognosis
Type: Publication Only
Background
Tumor microenvironment plays an important role in cancer initiation and progression. The cancer associated fibroblasts impact on resistance development to chemotherapeutic agents in the treatment of acute leukemia (AL) discussed.
Aims
The aim of the study was to determine the relation between colony-forming efficiency of bone marrow stromal fibroblasts, myelogram parameters and effectiveness of chemotherapy (CT) in children with lympho- and myelo- variants of acute leukemia (ALL, AML).
Methods
The study involved 38 children: ALL - 24, AML - 14. Age of children ranged from 6 to 18 years. Treatment was performed by standard protocols. Investigation of patients executed in complete remission phase after full chemotherapy course of primary diagnosis leukemia. Retrospective surveillance was conducted for 15 years. The effectiveness of chemotherapy evaluated by presence/absence of AL relapse. Among patients with ALL the relapse stated in 8 persons, among AML - in 7 children. Efficiency of bone marrow stromal fibroblast colony-forming units (E -CFUf) in vitro, myelogram performance were studied. Spearman correlation coefficient, U- test were used.
Results
E-CFUf not depend on the variant of acute leukemia (Uemp. = 61.5). In patients with AL we found the inverse correlation between the E-CFUf index and the percentage of erythroblasts (Rs = - 0,52) and monocytes (Rs = - 0,45) in the bone marrow. The direct connection between the number of monocytes and erythroblasts in myelogram defined (Rs = + 0,59), probably because of their joint operation in the development of erythroid elements of hematopoiesis. The inverse correlation( Rs = -0,44) between the percentage of monocytes in patients myelogram and AL relapse occurence was found.
Conclusion
The inverse correlation between stromal fibroblasts and erythroblasts as monocytes in the bone marrow of AL children indicates the regulatory role of stromal microenvironment elements in the functioning of hematopoiesis in the development of malignancy. Discovered correlation between relapse development and the number of monocytes in the AL patients bone marrow after end of protocol treatment opens up further possibilities to determine the mechanisms of chemoresistance formation at AL and review the ways to prognose the effectiveness of treatment of such patients.
Session topic: E-poster
Keyword(s): Chemoresistance, Leukemia, Microenvironment, Prognosis
{{ help_message }}
{{filter}}