COMPARISON BETWEEN AUTOMATED AND MANUAL INTERPHASE FLUORESCENT IN SITU HYBRIDIZATION (FISH) ANALYSIS OF T(9;22)(Q43;Q11.2)
(Abstract release date: 05/19/16)
EHA Library. Chaker H. 06/09/16; 134715; PB1815
Dr. Hend Chaker
Contributions
Contributions
Abstract
Abstract: PB1815
Type: Publication Only
Background
Metafer 4 or Metacyte is an automatic interphase FISH scoring system offered by MetaSystems. The scanning system contains a motorized microscope and a computer that achieves microscope control and real time image analysis.
Aims
Around the world, many laboratories have adopted Automated FISH scoring for interphase FISH analysis, however few have reported their experience. In our laboratory, we have validated automated FISH analysis for a number of probes, including BCR/ABL ES Dual Color Translocation (Abbott). Detection of t(9;22)(q34;q22) generating the BCR-ABL gene fusion by FISH is a tool available for clinical diagnosis and treatment monitoring of Chronic Myeloid Leukemia (CML).
Methods
Six different pools of leukemic blood cell suspensions were used as negative controls and 55 CML patient samples were analyzed by automatic FISH and manual analysis.
Results
A Cut off value of 0.8µm was set as maximum distance between signals for fusion count and 1.2µm as minimum spot distance for red and green spot count. Compared to manual read, the scored nuclei are categorized in a higher number of signal patterns with automated FISH analysis. In addition, the number of nuclei per category is different between the two methods. However, there was a strong correlation between automated and manual interpretation results. Only one case out of the 55 cases analyzed was discordant (1.8%), with results above normal reference range by Automated analysis, but within the normal range by Manual analysis.
Conclusion
Our scoring and interpretation criteria, as well as the advantages of the automated approach will be discussed.
Session topic: E-poster
Keyword(s): BCR-ABL, Chromosomal translocation, FISH
Type: Publication Only
Background
Metafer 4 or Metacyte is an automatic interphase FISH scoring system offered by MetaSystems. The scanning system contains a motorized microscope and a computer that achieves microscope control and real time image analysis.
Aims
Around the world, many laboratories have adopted Automated FISH scoring for interphase FISH analysis, however few have reported their experience. In our laboratory, we have validated automated FISH analysis for a number of probes, including BCR/ABL ES Dual Color Translocation (Abbott). Detection of t(9;22)(q34;q22) generating the BCR-ABL gene fusion by FISH is a tool available for clinical diagnosis and treatment monitoring of Chronic Myeloid Leukemia (CML).
Methods
Six different pools of leukemic blood cell suspensions were used as negative controls and 55 CML patient samples were analyzed by automatic FISH and manual analysis.
Results
A Cut off value of 0.8µm was set as maximum distance between signals for fusion count and 1.2µm as minimum spot distance for red and green spot count. Compared to manual read, the scored nuclei are categorized in a higher number of signal patterns with automated FISH analysis. In addition, the number of nuclei per category is different between the two methods. However, there was a strong correlation between automated and manual interpretation results. Only one case out of the 55 cases analyzed was discordant (1.8%), with results above normal reference range by Automated analysis, but within the normal range by Manual analysis.
Conclusion
Our scoring and interpretation criteria, as well as the advantages of the automated approach will be discussed.
Session topic: E-poster
Keyword(s): BCR-ABL, Chromosomal translocation, FISH
Abstract: PB1815
Type: Publication Only
Background
Metafer 4 or Metacyte is an automatic interphase FISH scoring system offered by MetaSystems. The scanning system contains a motorized microscope and a computer that achieves microscope control and real time image analysis.
Aims
Around the world, many laboratories have adopted Automated FISH scoring for interphase FISH analysis, however few have reported their experience. In our laboratory, we have validated automated FISH analysis for a number of probes, including BCR/ABL ES Dual Color Translocation (Abbott). Detection of t(9;22)(q34;q22) generating the BCR-ABL gene fusion by FISH is a tool available for clinical diagnosis and treatment monitoring of Chronic Myeloid Leukemia (CML).
Methods
Six different pools of leukemic blood cell suspensions were used as negative controls and 55 CML patient samples were analyzed by automatic FISH and manual analysis.
Results
A Cut off value of 0.8µm was set as maximum distance between signals for fusion count and 1.2µm as minimum spot distance for red and green spot count. Compared to manual read, the scored nuclei are categorized in a higher number of signal patterns with automated FISH analysis. In addition, the number of nuclei per category is different between the two methods. However, there was a strong correlation between automated and manual interpretation results. Only one case out of the 55 cases analyzed was discordant (1.8%), with results above normal reference range by Automated analysis, but within the normal range by Manual analysis.
Conclusion
Our scoring and interpretation criteria, as well as the advantages of the automated approach will be discussed.
Session topic: E-poster
Keyword(s): BCR-ABL, Chromosomal translocation, FISH
Type: Publication Only
Background
Metafer 4 or Metacyte is an automatic interphase FISH scoring system offered by MetaSystems. The scanning system contains a motorized microscope and a computer that achieves microscope control and real time image analysis.
Aims
Around the world, many laboratories have adopted Automated FISH scoring for interphase FISH analysis, however few have reported their experience. In our laboratory, we have validated automated FISH analysis for a number of probes, including BCR/ABL ES Dual Color Translocation (Abbott). Detection of t(9;22)(q34;q22) generating the BCR-ABL gene fusion by FISH is a tool available for clinical diagnosis and treatment monitoring of Chronic Myeloid Leukemia (CML).
Methods
Six different pools of leukemic blood cell suspensions were used as negative controls and 55 CML patient samples were analyzed by automatic FISH and manual analysis.
Results
A Cut off value of 0.8µm was set as maximum distance between signals for fusion count and 1.2µm as minimum spot distance for red and green spot count. Compared to manual read, the scored nuclei are categorized in a higher number of signal patterns with automated FISH analysis. In addition, the number of nuclei per category is different between the two methods. However, there was a strong correlation between automated and manual interpretation results. Only one case out of the 55 cases analyzed was discordant (1.8%), with results above normal reference range by Automated analysis, but within the normal range by Manual analysis.
Conclusion
Our scoring and interpretation criteria, as well as the advantages of the automated approach will be discussed.
Session topic: E-poster
Keyword(s): BCR-ABL, Chromosomal translocation, FISH
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