RARE RECURRENT CHROMOSOMAL ABNORMALITIES IN CLL DETECTED BY CONVENTIONAL CYTOGENETICS AFTER STIMULATION WITH CPG-ODN & IL-2 AND FISH
(Abstract release date: 05/19/16)
EHA Library. Stoklasova M. 06/09/16; 134669; PB1769
Martina Stoklasova
Contributions
Contributions
Abstract
Abstract: PB1769
Type: Publication Only
Background
Characteristic chromosomal abberations (i.e. 11q, 13q, 17p, and +12) play crucial role in chronic lymphocytic leukaemia (CLL) as important idenpendent prognostic factors. Combination of FISH and conventional chromosome banding (CBA) after stimulation with CpG oligonucleotides (CpG-ODN) and interleukin-2 (IL-2) allowed to capture increased number of chromosomal anormalities. We focused on rare recurrent chromosomal aberrations (RCA) that can provide new prognostic information in CLL.
Aims
Metaphase cytogenetic and molecular-cytogenetic analyses were performed in 766 CLL-patients from 2008 to 2015 (293 female, 473 male, median age at the time of analysis: 67 years) to evaluate the incidence of RCA.
Methods
CBA was performed on peripheral blood or bone marrow samples cultured in medium with stimulants. FISH was performed on unstimulated cells for detection of trisomy 12, del(11q), del(13q), del(17p). RCA were confirmed by interphase FISH (I-FISH), multicolor FISH or multicolor banding.
Results
A total of 714/766 (93,2 %) cases were succesfully stimulated for metaphase analysis. Chromosomal aberrations were detected in 600/714 (84,0%) of patients by combining both methods. RCA were detected in 144 cases ( 144/714, 20,2 %) and they were as follows: 3,5 % rearrangement of IGH (translocations partners 2, 4, 8, 9, 11, 12, 13, 16 and 19), 3,4 % gain of 2p, 3,4 % del(6q), 2,5 % del(14q), 1,7 % gain of 8q (MYC gene), 1,3 % del(8p), 1,3 % +18, 1,1 % +19, 0,6 % del(5q), 0,6 % t(18;22), 0,4 % gain of 12q, 0,4 % +21 and 0,1 % t(2;18).
Conclusion
Cytogenetic results are highly relevant in defining the prognosis of CLL patients. I-FISH as gold standard for detection of important chromosomal aberrations and CBA after stimulation with cocktail of CpG-ODN and IL-2 are efficient tools (methods) in routine diagnostic (analysis) of chromosomal aberrations. The importance of RCA in CLL will be discussed in the poster.
Session topic: E-poster
Keyword(s): Chromosomal abnormality
Type: Publication Only
Background
Characteristic chromosomal abberations (i.e. 11q, 13q, 17p, and +12) play crucial role in chronic lymphocytic leukaemia (CLL) as important idenpendent prognostic factors. Combination of FISH and conventional chromosome banding (CBA) after stimulation with CpG oligonucleotides (CpG-ODN) and interleukin-2 (IL-2) allowed to capture increased number of chromosomal anormalities. We focused on rare recurrent chromosomal aberrations (RCA) that can provide new prognostic information in CLL.
Aims
Metaphase cytogenetic and molecular-cytogenetic analyses were performed in 766 CLL-patients from 2008 to 2015 (293 female, 473 male, median age at the time of analysis: 67 years) to evaluate the incidence of RCA.
Methods
CBA was performed on peripheral blood or bone marrow samples cultured in medium with stimulants. FISH was performed on unstimulated cells for detection of trisomy 12, del(11q), del(13q), del(17p). RCA were confirmed by interphase FISH (I-FISH), multicolor FISH or multicolor banding.
Results
A total of 714/766 (93,2 %) cases were succesfully stimulated for metaphase analysis. Chromosomal aberrations were detected in 600/714 (84,0%) of patients by combining both methods. RCA were detected in 144 cases ( 144/714, 20,2 %) and they were as follows: 3,5 % rearrangement of IGH (translocations partners 2, 4, 8, 9, 11, 12, 13, 16 and 19), 3,4 % gain of 2p, 3,4 % del(6q), 2,5 % del(14q), 1,7 % gain of 8q (MYC gene), 1,3 % del(8p), 1,3 % +18, 1,1 % +19, 0,6 % del(5q), 0,6 % t(18;22), 0,4 % gain of 12q, 0,4 % +21 and 0,1 % t(2;18).
Conclusion
Cytogenetic results are highly relevant in defining the prognosis of CLL patients. I-FISH as gold standard for detection of important chromosomal aberrations and CBA after stimulation with cocktail of CpG-ODN and IL-2 are efficient tools (methods) in routine diagnostic (analysis) of chromosomal aberrations. The importance of RCA in CLL will be discussed in the poster.
Session topic: E-poster
Keyword(s): Chromosomal abnormality
Abstract: PB1769
Type: Publication Only
Background
Characteristic chromosomal abberations (i.e. 11q, 13q, 17p, and +12) play crucial role in chronic lymphocytic leukaemia (CLL) as important idenpendent prognostic factors. Combination of FISH and conventional chromosome banding (CBA) after stimulation with CpG oligonucleotides (CpG-ODN) and interleukin-2 (IL-2) allowed to capture increased number of chromosomal anormalities. We focused on rare recurrent chromosomal aberrations (RCA) that can provide new prognostic information in CLL.
Aims
Metaphase cytogenetic and molecular-cytogenetic analyses were performed in 766 CLL-patients from 2008 to 2015 (293 female, 473 male, median age at the time of analysis: 67 years) to evaluate the incidence of RCA.
Methods
CBA was performed on peripheral blood or bone marrow samples cultured in medium with stimulants. FISH was performed on unstimulated cells for detection of trisomy 12, del(11q), del(13q), del(17p). RCA were confirmed by interphase FISH (I-FISH), multicolor FISH or multicolor banding.
Results
A total of 714/766 (93,2 %) cases were succesfully stimulated for metaphase analysis. Chromosomal aberrations were detected in 600/714 (84,0%) of patients by combining both methods. RCA were detected in 144 cases ( 144/714, 20,2 %) and they were as follows: 3,5 % rearrangement of IGH (translocations partners 2, 4, 8, 9, 11, 12, 13, 16 and 19), 3,4 % gain of 2p, 3,4 % del(6q), 2,5 % del(14q), 1,7 % gain of 8q (MYC gene), 1,3 % del(8p), 1,3 % +18, 1,1 % +19, 0,6 % del(5q), 0,6 % t(18;22), 0,4 % gain of 12q, 0,4 % +21 and 0,1 % t(2;18).
Conclusion
Cytogenetic results are highly relevant in defining the prognosis of CLL patients. I-FISH as gold standard for detection of important chromosomal aberrations and CBA after stimulation with cocktail of CpG-ODN and IL-2 are efficient tools (methods) in routine diagnostic (analysis) of chromosomal aberrations. The importance of RCA in CLL will be discussed in the poster.
Session topic: E-poster
Keyword(s): Chromosomal abnormality
Type: Publication Only
Background
Characteristic chromosomal abberations (i.e. 11q, 13q, 17p, and +12) play crucial role in chronic lymphocytic leukaemia (CLL) as important idenpendent prognostic factors. Combination of FISH and conventional chromosome banding (CBA) after stimulation with CpG oligonucleotides (CpG-ODN) and interleukin-2 (IL-2) allowed to capture increased number of chromosomal anormalities. We focused on rare recurrent chromosomal aberrations (RCA) that can provide new prognostic information in CLL.
Aims
Metaphase cytogenetic and molecular-cytogenetic analyses were performed in 766 CLL-patients from 2008 to 2015 (293 female, 473 male, median age at the time of analysis: 67 years) to evaluate the incidence of RCA.
Methods
CBA was performed on peripheral blood or bone marrow samples cultured in medium with stimulants. FISH was performed on unstimulated cells for detection of trisomy 12, del(11q), del(13q), del(17p). RCA were confirmed by interphase FISH (I-FISH), multicolor FISH or multicolor banding.
Results
A total of 714/766 (93,2 %) cases were succesfully stimulated for metaphase analysis. Chromosomal aberrations were detected in 600/714 (84,0%) of patients by combining both methods. RCA were detected in 144 cases ( 144/714, 20,2 %) and they were as follows: 3,5 % rearrangement of IGH (translocations partners 2, 4, 8, 9, 11, 12, 13, 16 and 19), 3,4 % gain of 2p, 3,4 % del(6q), 2,5 % del(14q), 1,7 % gain of 8q (MYC gene), 1,3 % del(8p), 1,3 % +18, 1,1 % +19, 0,6 % del(5q), 0,6 % t(18;22), 0,4 % gain of 12q, 0,4 % +21 and 0,1 % t(2;18).
Conclusion
Cytogenetic results are highly relevant in defining the prognosis of CLL patients. I-FISH as gold standard for detection of important chromosomal aberrations and CBA after stimulation with cocktail of CpG-ODN and IL-2 are efficient tools (methods) in routine diagnostic (analysis) of chromosomal aberrations. The importance of RCA in CLL will be discussed in the poster.
Session topic: E-poster
Keyword(s): Chromosomal abnormality
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