THE POTENTIAL EFFECT OF CATHEPSIN B ON THE CLINICAL COURSE OF CHILDHOOD ALL
(Abstract release date: 05/19/16)
EHA Library. Zalewska-Szewczyk B. 06/09/16; 134514; PB1614
Dr. Beata Zalewska-Szewczyk
Contributions
Contributions
Abstract
Abstract: PB1614
Type: Publication Only
Background
Cathepsins belong to the ubiquitous family of lysosomal cysteine proteases responsible for the conversion and degradation of intracellular proteins. In many types of cancer increased overexpression of cathepsin B (CTSB) was observed, in some solid tumors, overexpression of cathepsin B and L is considered a negative prognostic factor. Published pilot data showing a relationship between an increased expression of cathepsin B and L (CTSB, CTSL) and a worse outcome in acute myeloblastic leukemia.
Aims
The aim of this study was to evaluate the potential significance of CTSB expression in childhood ALL.
Methods
CTSB expression was evaluated in bone marrow cells collected at the time of diagnosis in 32 children aged 1.4 to 17.9 years, mean 7.16, 17 boys with newly diagnosed acute lymphoblastic leukemia who were treated at a single center according the protocol ALL IC 2002. In the bone marrow samples taken at the diagnosis RNA was isolated, in a further step of reverse transcription reactions were carried out using a commercially available kit (Applied Biosystems, USA). Determination of the expression was performed by real-time PCR.
Results
For the further analysis the expression above the upper quartile was defined as high. It was observed that the children with high CTSB expression were diagnosed at a younger age (median 6.51 vs 2.41 years, p=0.047). There was no correlation between the level of expression of CTSB in leukemia cells and WBC at baseline (p=0.31). The CTSB expression level in leukemia cells had no effect on the activity of L-asparaginase after the first dose (median of 89.42 IU/L vs 93 IU/L, p=0.226), early response (status bone marrow 15th day of induction, p=0.256), the response to steroid therapy (p = 0.53) and the status of the CNS (p = 0.37).
Conclusion
In summary, this pilot study may suggest the potential importance of CTSB in acute lymphoblastic leukemia in children. Further analysis in a larger study group is needed.
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Children
Type: Publication Only
Background
Cathepsins belong to the ubiquitous family of lysosomal cysteine proteases responsible for the conversion and degradation of intracellular proteins. In many types of cancer increased overexpression of cathepsin B (CTSB) was observed, in some solid tumors, overexpression of cathepsin B and L is considered a negative prognostic factor. Published pilot data showing a relationship between an increased expression of cathepsin B and L (CTSB, CTSL) and a worse outcome in acute myeloblastic leukemia.
Aims
The aim of this study was to evaluate the potential significance of CTSB expression in childhood ALL.
Methods
CTSB expression was evaluated in bone marrow cells collected at the time of diagnosis in 32 children aged 1.4 to 17.9 years, mean 7.16, 17 boys with newly diagnosed acute lymphoblastic leukemia who were treated at a single center according the protocol ALL IC 2002. In the bone marrow samples taken at the diagnosis RNA was isolated, in a further step of reverse transcription reactions were carried out using a commercially available kit (Applied Biosystems, USA). Determination of the expression was performed by real-time PCR.
Results
For the further analysis the expression above the upper quartile was defined as high. It was observed that the children with high CTSB expression were diagnosed at a younger age (median 6.51 vs 2.41 years, p=0.047). There was no correlation between the level of expression of CTSB in leukemia cells and WBC at baseline (p=0.31). The CTSB expression level in leukemia cells had no effect on the activity of L-asparaginase after the first dose (median of 89.42 IU/L vs 93 IU/L, p=0.226), early response (status bone marrow 15th day of induction, p=0.256), the response to steroid therapy (p = 0.53) and the status of the CNS (p = 0.37).
Conclusion
In summary, this pilot study may suggest the potential importance of CTSB in acute lymphoblastic leukemia in children. Further analysis in a larger study group is needed.
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Children
Abstract: PB1614
Type: Publication Only
Background
Cathepsins belong to the ubiquitous family of lysosomal cysteine proteases responsible for the conversion and degradation of intracellular proteins. In many types of cancer increased overexpression of cathepsin B (CTSB) was observed, in some solid tumors, overexpression of cathepsin B and L is considered a negative prognostic factor. Published pilot data showing a relationship between an increased expression of cathepsin B and L (CTSB, CTSL) and a worse outcome in acute myeloblastic leukemia.
Aims
The aim of this study was to evaluate the potential significance of CTSB expression in childhood ALL.
Methods
CTSB expression was evaluated in bone marrow cells collected at the time of diagnosis in 32 children aged 1.4 to 17.9 years, mean 7.16, 17 boys with newly diagnosed acute lymphoblastic leukemia who were treated at a single center according the protocol ALL IC 2002. In the bone marrow samples taken at the diagnosis RNA was isolated, in a further step of reverse transcription reactions were carried out using a commercially available kit (Applied Biosystems, USA). Determination of the expression was performed by real-time PCR.
Results
For the further analysis the expression above the upper quartile was defined as high. It was observed that the children with high CTSB expression were diagnosed at a younger age (median 6.51 vs 2.41 years, p=0.047). There was no correlation between the level of expression of CTSB in leukemia cells and WBC at baseline (p=0.31). The CTSB expression level in leukemia cells had no effect on the activity of L-asparaginase after the first dose (median of 89.42 IU/L vs 93 IU/L, p=0.226), early response (status bone marrow 15th day of induction, p=0.256), the response to steroid therapy (p = 0.53) and the status of the CNS (p = 0.37).
Conclusion
In summary, this pilot study may suggest the potential importance of CTSB in acute lymphoblastic leukemia in children. Further analysis in a larger study group is needed.
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Children
Type: Publication Only
Background
Cathepsins belong to the ubiquitous family of lysosomal cysteine proteases responsible for the conversion and degradation of intracellular proteins. In many types of cancer increased overexpression of cathepsin B (CTSB) was observed, in some solid tumors, overexpression of cathepsin B and L is considered a negative prognostic factor. Published pilot data showing a relationship between an increased expression of cathepsin B and L (CTSB, CTSL) and a worse outcome in acute myeloblastic leukemia.
Aims
The aim of this study was to evaluate the potential significance of CTSB expression in childhood ALL.
Methods
CTSB expression was evaluated in bone marrow cells collected at the time of diagnosis in 32 children aged 1.4 to 17.9 years, mean 7.16, 17 boys with newly diagnosed acute lymphoblastic leukemia who were treated at a single center according the protocol ALL IC 2002. In the bone marrow samples taken at the diagnosis RNA was isolated, in a further step of reverse transcription reactions were carried out using a commercially available kit (Applied Biosystems, USA). Determination of the expression was performed by real-time PCR.
Results
For the further analysis the expression above the upper quartile was defined as high. It was observed that the children with high CTSB expression were diagnosed at a younger age (median 6.51 vs 2.41 years, p=0.047). There was no correlation between the level of expression of CTSB in leukemia cells and WBC at baseline (p=0.31). The CTSB expression level in leukemia cells had no effect on the activity of L-asparaginase after the first dose (median of 89.42 IU/L vs 93 IU/L, p=0.226), early response (status bone marrow 15th day of induction, p=0.256), the response to steroid therapy (p = 0.53) and the status of the CNS (p = 0.37).
Conclusion
In summary, this pilot study may suggest the potential importance of CTSB in acute lymphoblastic leukemia in children. Further analysis in a larger study group is needed.
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Children
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