SERUM PROFILE OF CYTOKINES, CYTOKINE RECEPTORS AND ADHESION MOLECULES IN PATIENTS WITH NEWLY DIAGNOSED ACUTE LYMPHOBLASTIC LEUKEMIA AND IN HEALTHY SUBJECTS
(Abstract release date: 05/19/16)
EHA Library. Horacek J. 06/09/16; 134500; PB1600
Prof. Dr. Jan M Horacek
Contributions
Contributions
Abstract
Abstract: PB1600
Type: Publication Only
Background
Cytokines and adhesion molecules have been studied as markers of immune system activation in various diseases including hematological malignancies. The knowledge gained from multiple cytokine and adhesion molecule analysis could allow better diagnosis and disease management.
Aims
The aim of our study was to evaluate serum profile of cytokines, cytokine receptors and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia (ALL) and in healthy subjects. Correlations between analytes were evaluated separately in both groups.
Methods
Serum samples of 30 newly diagnosed ALL patients (median age 46, range 22–75 years, 20 males) and 15 healthy subjects (median age 41, range 25–58 years, 11 males) were analyzed. We evaluated serum levels of 31 analytes, specifically 21 cytokines, 4 soluble cytokine receptors, 5 soluble adhesion molecules and Matrix Metalloproteinase-9. From cytokines, we measured Interleukins (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-15, IL-23), Epidermal Growth Factor (EGF), Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Interferon-γ (IFN-γ), Macrophage Inflammatory Protein-1α (MIP-1α), Monocyte Chemotactic Protein-1 (MCP-1), Tumour Necrosis Factor-α (TNF-α), Vascular Endothelial Growth Factor (VEGF) and soluble receptors for IL-2 (sIL-2Rα), IL-6 (sIL-6R), TNF-α type I and II (sTNFR-1,2). From soluble adhesion molecules, we measured E-Selectin (E-SEL), L-Selectin (L-SEL), P-Selectin (P-SEL), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1). All analytes were measured by biochip array technology on Evidence Investigator analyzer (Randox). Statistical evaluation was done by a professional statistician using software R 3.2.3 (R Core Team 2015). Probability values (p) < 0.05 were considered statistically significant.
Results
Comparing cytokine and adhesion molecule levels in newly diagnosed ALL and healthy subjects, we found significant increase in ALL in serum levels of IL-6, IL-8, IL-15, MIP-1α, MCP-1, sIL-2Rα, sIL-6R, sTNFR-1, sTNFR-2, L-SEL, ICAM-1 and VCAM-1 (p<0.01). Furthermore, we found in ALL significant decrease in serum levels of IL-3, IL-4 and GM-CSF (p<0.01). Serum levels of other evaluated analytes were without significant differences. In the group of ALL patients, we found statistically significant correlations between sTNFR-1 and sTNFR-2 (r=0.805; p<0.0001), IL-1α and IL-4 (r=0.700; p=0.008), sTNFR-2 and MIP-1α (r=0.657; p=0.037), sTNFR-2 and VCAM-1 (r=0.652; p=0.044). In the control group of healthy subjects, we found statistically significant correlations between EGF and IL-7 (r=0.876; p=0.009), EGF and IL-8 (r=0.856; p=0.022). Other correlations between analytes did not reach statistical significance.
Conclusion
Our results show that serum levels of some cytokines, cytokine receptors and adhesion molecules are significantly altered in patients with newly diagnosed ALL, reflecting activity of the disease. We found statistically significant correlations between some analytes within ALL and control group. Further studies are needed to establish if the alterations observed in the levels of these molecules could be used as a clinically relevant biomarker for ALL.The work was supported by a long-term organisation development plan 1011 (FMHS).
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Adhesion, Cytokine
Type: Publication Only
Background
Cytokines and adhesion molecules have been studied as markers of immune system activation in various diseases including hematological malignancies. The knowledge gained from multiple cytokine and adhesion molecule analysis could allow better diagnosis and disease management.
Aims
The aim of our study was to evaluate serum profile of cytokines, cytokine receptors and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia (ALL) and in healthy subjects. Correlations between analytes were evaluated separately in both groups.
Methods
Serum samples of 30 newly diagnosed ALL patients (median age 46, range 22–75 years, 20 males) and 15 healthy subjects (median age 41, range 25–58 years, 11 males) were analyzed. We evaluated serum levels of 31 analytes, specifically 21 cytokines, 4 soluble cytokine receptors, 5 soluble adhesion molecules and Matrix Metalloproteinase-9. From cytokines, we measured Interleukins (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-15, IL-23), Epidermal Growth Factor (EGF), Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Interferon-γ (IFN-γ), Macrophage Inflammatory Protein-1α (MIP-1α), Monocyte Chemotactic Protein-1 (MCP-1), Tumour Necrosis Factor-α (TNF-α), Vascular Endothelial Growth Factor (VEGF) and soluble receptors for IL-2 (sIL-2Rα), IL-6 (sIL-6R), TNF-α type I and II (sTNFR-1,2). From soluble adhesion molecules, we measured E-Selectin (E-SEL), L-Selectin (L-SEL), P-Selectin (P-SEL), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1). All analytes were measured by biochip array technology on Evidence Investigator analyzer (Randox). Statistical evaluation was done by a professional statistician using software R 3.2.3 (R Core Team 2015). Probability values (p) < 0.05 were considered statistically significant.
Results
Comparing cytokine and adhesion molecule levels in newly diagnosed ALL and healthy subjects, we found significant increase in ALL in serum levels of IL-6, IL-8, IL-15, MIP-1α, MCP-1, sIL-2Rα, sIL-6R, sTNFR-1, sTNFR-2, L-SEL, ICAM-1 and VCAM-1 (p<0.01). Furthermore, we found in ALL significant decrease in serum levels of IL-3, IL-4 and GM-CSF (p<0.01). Serum levels of other evaluated analytes were without significant differences. In the group of ALL patients, we found statistically significant correlations between sTNFR-1 and sTNFR-2 (r=0.805; p<0.0001), IL-1α and IL-4 (r=0.700; p=0.008), sTNFR-2 and MIP-1α (r=0.657; p=0.037), sTNFR-2 and VCAM-1 (r=0.652; p=0.044). In the control group of healthy subjects, we found statistically significant correlations between EGF and IL-7 (r=0.876; p=0.009), EGF and IL-8 (r=0.856; p=0.022). Other correlations between analytes did not reach statistical significance.
Conclusion
Our results show that serum levels of some cytokines, cytokine receptors and adhesion molecules are significantly altered in patients with newly diagnosed ALL, reflecting activity of the disease. We found statistically significant correlations between some analytes within ALL and control group. Further studies are needed to establish if the alterations observed in the levels of these molecules could be used as a clinically relevant biomarker for ALL.The work was supported by a long-term organisation development plan 1011 (FMHS).
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Adhesion, Cytokine
Abstract: PB1600
Type: Publication Only
Background
Cytokines and adhesion molecules have been studied as markers of immune system activation in various diseases including hematological malignancies. The knowledge gained from multiple cytokine and adhesion molecule analysis could allow better diagnosis and disease management.
Aims
The aim of our study was to evaluate serum profile of cytokines, cytokine receptors and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia (ALL) and in healthy subjects. Correlations between analytes were evaluated separately in both groups.
Methods
Serum samples of 30 newly diagnosed ALL patients (median age 46, range 22–75 years, 20 males) and 15 healthy subjects (median age 41, range 25–58 years, 11 males) were analyzed. We evaluated serum levels of 31 analytes, specifically 21 cytokines, 4 soluble cytokine receptors, 5 soluble adhesion molecules and Matrix Metalloproteinase-9. From cytokines, we measured Interleukins (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-15, IL-23), Epidermal Growth Factor (EGF), Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Interferon-γ (IFN-γ), Macrophage Inflammatory Protein-1α (MIP-1α), Monocyte Chemotactic Protein-1 (MCP-1), Tumour Necrosis Factor-α (TNF-α), Vascular Endothelial Growth Factor (VEGF) and soluble receptors for IL-2 (sIL-2Rα), IL-6 (sIL-6R), TNF-α type I and II (sTNFR-1,2). From soluble adhesion molecules, we measured E-Selectin (E-SEL), L-Selectin (L-SEL), P-Selectin (P-SEL), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1). All analytes were measured by biochip array technology on Evidence Investigator analyzer (Randox). Statistical evaluation was done by a professional statistician using software R 3.2.3 (R Core Team 2015). Probability values (p) < 0.05 were considered statistically significant.
Results
Comparing cytokine and adhesion molecule levels in newly diagnosed ALL and healthy subjects, we found significant increase in ALL in serum levels of IL-6, IL-8, IL-15, MIP-1α, MCP-1, sIL-2Rα, sIL-6R, sTNFR-1, sTNFR-2, L-SEL, ICAM-1 and VCAM-1 (p<0.01). Furthermore, we found in ALL significant decrease in serum levels of IL-3, IL-4 and GM-CSF (p<0.01). Serum levels of other evaluated analytes were without significant differences. In the group of ALL patients, we found statistically significant correlations between sTNFR-1 and sTNFR-2 (r=0.805; p<0.0001), IL-1α and IL-4 (r=0.700; p=0.008), sTNFR-2 and MIP-1α (r=0.657; p=0.037), sTNFR-2 and VCAM-1 (r=0.652; p=0.044). In the control group of healthy subjects, we found statistically significant correlations between EGF and IL-7 (r=0.876; p=0.009), EGF and IL-8 (r=0.856; p=0.022). Other correlations between analytes did not reach statistical significance.
Conclusion
Our results show that serum levels of some cytokines, cytokine receptors and adhesion molecules are significantly altered in patients with newly diagnosed ALL, reflecting activity of the disease. We found statistically significant correlations between some analytes within ALL and control group. Further studies are needed to establish if the alterations observed in the levels of these molecules could be used as a clinically relevant biomarker for ALL.The work was supported by a long-term organisation development plan 1011 (FMHS).
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Adhesion, Cytokine
Type: Publication Only
Background
Cytokines and adhesion molecules have been studied as markers of immune system activation in various diseases including hematological malignancies. The knowledge gained from multiple cytokine and adhesion molecule analysis could allow better diagnosis and disease management.
Aims
The aim of our study was to evaluate serum profile of cytokines, cytokine receptors and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia (ALL) and in healthy subjects. Correlations between analytes were evaluated separately in both groups.
Methods
Serum samples of 30 newly diagnosed ALL patients (median age 46, range 22–75 years, 20 males) and 15 healthy subjects (median age 41, range 25–58 years, 11 males) were analyzed. We evaluated serum levels of 31 analytes, specifically 21 cytokines, 4 soluble cytokine receptors, 5 soluble adhesion molecules and Matrix Metalloproteinase-9. From cytokines, we measured Interleukins (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-15, IL-23), Epidermal Growth Factor (EGF), Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Interferon-γ (IFN-γ), Macrophage Inflammatory Protein-1α (MIP-1α), Monocyte Chemotactic Protein-1 (MCP-1), Tumour Necrosis Factor-α (TNF-α), Vascular Endothelial Growth Factor (VEGF) and soluble receptors for IL-2 (sIL-2Rα), IL-6 (sIL-6R), TNF-α type I and II (sTNFR-1,2). From soluble adhesion molecules, we measured E-Selectin (E-SEL), L-Selectin (L-SEL), P-Selectin (P-SEL), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1). All analytes were measured by biochip array technology on Evidence Investigator analyzer (Randox). Statistical evaluation was done by a professional statistician using software R 3.2.3 (R Core Team 2015). Probability values (p) < 0.05 were considered statistically significant.
Results
Comparing cytokine and adhesion molecule levels in newly diagnosed ALL and healthy subjects, we found significant increase in ALL in serum levels of IL-6, IL-8, IL-15, MIP-1α, MCP-1, sIL-2Rα, sIL-6R, sTNFR-1, sTNFR-2, L-SEL, ICAM-1 and VCAM-1 (p<0.01). Furthermore, we found in ALL significant decrease in serum levels of IL-3, IL-4 and GM-CSF (p<0.01). Serum levels of other evaluated analytes were without significant differences. In the group of ALL patients, we found statistically significant correlations between sTNFR-1 and sTNFR-2 (r=0.805; p<0.0001), IL-1α and IL-4 (r=0.700; p=0.008), sTNFR-2 and MIP-1α (r=0.657; p=0.037), sTNFR-2 and VCAM-1 (r=0.652; p=0.044). In the control group of healthy subjects, we found statistically significant correlations between EGF and IL-7 (r=0.876; p=0.009), EGF and IL-8 (r=0.856; p=0.022). Other correlations between analytes did not reach statistical significance.
Conclusion
Our results show that serum levels of some cytokines, cytokine receptors and adhesion molecules are significantly altered in patients with newly diagnosed ALL, reflecting activity of the disease. We found statistically significant correlations between some analytes within ALL and control group. Further studies are needed to establish if the alterations observed in the levels of these molecules could be used as a clinically relevant biomarker for ALL.The work was supported by a long-term organisation development plan 1011 (FMHS).
Session topic: E-poster
Keyword(s): Acute lymphoblastic leukemia, Adhesion, Cytokine
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