A NEW METHOD FOR ACTIVATING PLATELETS IN PLATELET-RICH PLASMA TO USE IN REGENERATIVE MEDICINE: A CYCLE OF FREEZING AND THAWING
(Abstract release date: 05/19/16)
EHA Library. Gursel O. 06/09/16; 133122; E1573
Assoc. Prof. Orhan Gursel
Contributions
Contributions
Abstract
Abstract: E1573
Type: Eposter Presentation
Background
Platelet-rich plasma (PRP) is a platelet-derived product, which exhibits regenerative properties and have been widely used in regenerative medicine. Platelet-rich plasma can be used with or without previous platelet activation, for what purpose is to be used. There are different ways of activating platelets. Activating platelets by freezing cycles is a new, not widely known and applied method.
Aims
The objective of the study was to assess whether and to which amount a cycle of freezing/thawing may affect the amount of growth factors in autologous platelet-rich plasma.
Methods
Fifteen ml of whole blood was obtained by venipuncture and collected in two citrated tubes from 20 healthy, male volunteer donors to obtain PRP. After preparation of PRP with standard methods, they were divided into two groups. First group was activated by adding 10% calcium gluconate to finalize PRP with volume ratio of 1:10. Second group was cryopreserved at -80°C for 24 hours. Of the obtained PRP, insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB), basic fibroblast growth factor (bFGF) concentrations were measures using the ELISA kit following the manufacturer’s instructions.
Results
The mean platelet count of the donors and the autologous PRPs were 238,5 ± 44,7 x 103 / µl and 544,7 ± 161,5 x 103 / µl, respectively. Overall, there was an increase in the concentration of the insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB) and basic fibroblast growth factor (bFGF) after a cycle of freezing and thawing. But only the increase in PDGF levels were significant (218,90 ± 30 and 78,95 ± 49,42 respectively; p: 0,0001). There was not a statistically significant difference between the GF levels of freeze thawed and calcium activated PRPs. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in multivariate model. In summary overall, there was an increase in the concentration of the three GFs, but only PDGF was significant. There was not a significant difference between the GF levels of post-freezed and calcium activated PRP. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in the multivariate model.
Conclusion
With its features of being simple, inexpensive and easy for standardization; a cycle of freezing/thawing may be the method of choice in the future for PRP activation procedure, especially for obtaining PRPs rich in PDGF, without inducing fibrin matrix. This study is done with the support of the Turkish Society of Hematology, with the approval number of 2014-8.
Session topic: E-poster
Keyword(s): Plasma, Platelet activation
Type: Eposter Presentation
Background
Platelet-rich plasma (PRP) is a platelet-derived product, which exhibits regenerative properties and have been widely used in regenerative medicine. Platelet-rich plasma can be used with or without previous platelet activation, for what purpose is to be used. There are different ways of activating platelets. Activating platelets by freezing cycles is a new, not widely known and applied method.
Aims
The objective of the study was to assess whether and to which amount a cycle of freezing/thawing may affect the amount of growth factors in autologous platelet-rich plasma.
Methods
Fifteen ml of whole blood was obtained by venipuncture and collected in two citrated tubes from 20 healthy, male volunteer donors to obtain PRP. After preparation of PRP with standard methods, they were divided into two groups. First group was activated by adding 10% calcium gluconate to finalize PRP with volume ratio of 1:10. Second group was cryopreserved at -80°C for 24 hours. Of the obtained PRP, insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB), basic fibroblast growth factor (bFGF) concentrations were measures using the ELISA kit following the manufacturer’s instructions.
Results
The mean platelet count of the donors and the autologous PRPs were 238,5 ± 44,7 x 103 / µl and 544,7 ± 161,5 x 103 / µl, respectively. Overall, there was an increase in the concentration of the insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB) and basic fibroblast growth factor (bFGF) after a cycle of freezing and thawing. But only the increase in PDGF levels were significant (218,90 ± 30 and 78,95 ± 49,42 respectively; p: 0,0001). There was not a statistically significant difference between the GF levels of freeze thawed and calcium activated PRPs. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in multivariate model. In summary overall, there was an increase in the concentration of the three GFs, but only PDGF was significant. There was not a significant difference between the GF levels of post-freezed and calcium activated PRP. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in the multivariate model.
Conclusion
With its features of being simple, inexpensive and easy for standardization; a cycle of freezing/thawing may be the method of choice in the future for PRP activation procedure, especially for obtaining PRPs rich in PDGF, without inducing fibrin matrix. This study is done with the support of the Turkish Society of Hematology, with the approval number of 2014-8.
Session topic: E-poster
Keyword(s): Plasma, Platelet activation
Abstract: E1573
Type: Eposter Presentation
Background
Platelet-rich plasma (PRP) is a platelet-derived product, which exhibits regenerative properties and have been widely used in regenerative medicine. Platelet-rich plasma can be used with or without previous platelet activation, for what purpose is to be used. There are different ways of activating platelets. Activating platelets by freezing cycles is a new, not widely known and applied method.
Aims
The objective of the study was to assess whether and to which amount a cycle of freezing/thawing may affect the amount of growth factors in autologous platelet-rich plasma.
Methods
Fifteen ml of whole blood was obtained by venipuncture and collected in two citrated tubes from 20 healthy, male volunteer donors to obtain PRP. After preparation of PRP with standard methods, they were divided into two groups. First group was activated by adding 10% calcium gluconate to finalize PRP with volume ratio of 1:10. Second group was cryopreserved at -80°C for 24 hours. Of the obtained PRP, insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB), basic fibroblast growth factor (bFGF) concentrations were measures using the ELISA kit following the manufacturer’s instructions.
Results
The mean platelet count of the donors and the autologous PRPs were 238,5 ± 44,7 x 103 / µl and 544,7 ± 161,5 x 103 / µl, respectively. Overall, there was an increase in the concentration of the insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB) and basic fibroblast growth factor (bFGF) after a cycle of freezing and thawing. But only the increase in PDGF levels were significant (218,90 ± 30 and 78,95 ± 49,42 respectively; p: 0,0001). There was not a statistically significant difference between the GF levels of freeze thawed and calcium activated PRPs. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in multivariate model. In summary overall, there was an increase in the concentration of the three GFs, but only PDGF was significant. There was not a significant difference between the GF levels of post-freezed and calcium activated PRP. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in the multivariate model.
Conclusion
With its features of being simple, inexpensive and easy for standardization; a cycle of freezing/thawing may be the method of choice in the future for PRP activation procedure, especially for obtaining PRPs rich in PDGF, without inducing fibrin matrix. This study is done with the support of the Turkish Society of Hematology, with the approval number of 2014-8.
Session topic: E-poster
Keyword(s): Plasma, Platelet activation
Type: Eposter Presentation
Background
Platelet-rich plasma (PRP) is a platelet-derived product, which exhibits regenerative properties and have been widely used in regenerative medicine. Platelet-rich plasma can be used with or without previous platelet activation, for what purpose is to be used. There are different ways of activating platelets. Activating platelets by freezing cycles is a new, not widely known and applied method.
Aims
The objective of the study was to assess whether and to which amount a cycle of freezing/thawing may affect the amount of growth factors in autologous platelet-rich plasma.
Methods
Fifteen ml of whole blood was obtained by venipuncture and collected in two citrated tubes from 20 healthy, male volunteer donors to obtain PRP. After preparation of PRP with standard methods, they were divided into two groups. First group was activated by adding 10% calcium gluconate to finalize PRP with volume ratio of 1:10. Second group was cryopreserved at -80°C for 24 hours. Of the obtained PRP, insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB), basic fibroblast growth factor (bFGF) concentrations were measures using the ELISA kit following the manufacturer’s instructions.
Results
The mean platelet count of the donors and the autologous PRPs were 238,5 ± 44,7 x 103 / µl and 544,7 ± 161,5 x 103 / µl, respectively. Overall, there was an increase in the concentration of the insulin-like growth factor-1 (IGF-1) platelet-derived growth factor (PDGF-BB) and basic fibroblast growth factor (bFGF) after a cycle of freezing and thawing. But only the increase in PDGF levels were significant (218,90 ± 30 and 78,95 ± 49,42 respectively; p: 0,0001). There was not a statistically significant difference between the GF levels of freeze thawed and calcium activated PRPs. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in multivariate model. In summary overall, there was an increase in the concentration of the three GFs, but only PDGF was significant. There was not a significant difference between the GF levels of post-freezed and calcium activated PRP. A cycle of freezing/thawing was the only independent factor associated with growth factor yield in the multivariate model.
Conclusion
With its features of being simple, inexpensive and easy for standardization; a cycle of freezing/thawing may be the method of choice in the future for PRP activation procedure, especially for obtaining PRPs rich in PDGF, without inducing fibrin matrix. This study is done with the support of the Turkish Society of Hematology, with the approval number of 2014-8.
Session topic: E-poster
Keyword(s): Plasma, Platelet activation
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