VASCULAR ENDOTHELIAL CELL-RELATING MOLECULES EXPRESSED IN AN ADULT HUMAN DERMAL FIBROBLAST WHEN CULTURED WITH INTERLEUKIN-1-BETA
(Abstract release date: 05/19/16)
EHA Library. Sumiyoshi R. 06/09/16; 132688; E1139
Dr. Ritsu Sumiyoshi
Contributions
Contributions
Abstract
Abstract: E1139
Type: Eposter Presentation
Background
We previously reported that when an adult human dermal fibroblast (HDF) was cultured with interleukin (IL)-1-beta (b), vascular endothelial growth factor (VEGF)-A, erythropoietin (EPO), anti-human VEGF-C antibody (Ab) and anti-human tumor necrosis factor (TNF)-alpha (a) Ab, hematopoiesis-related genes expressed; however, culturing cells were morphologically fibroblasts (56th ASH). We also reported that hemogenic endothelium-relating molecules expressed in HDF with poly (I:C) culture and an electrical stimulation (44th ISEH).
Aims
In this report we observed the biological effects of IL-1-b to HDF for up to one month-culture.
Methods
HDF was cultured with CELRENA medium, used for induced pluripotent stem (iPS) cell-culture, on 0.1 % gelatin-coated plate (porcine skin). Cultures were added with recombinant human IL-1-b for up to four weeks with splitting each one week. Morphological changes and the expression of vascular endothelial cell-related genes, and hematopoiesis-related ones were observed.
Results
When HDF was cultured with IL-1-b, CD31, CD34, and CD41 molecules expressed for two weeks’ culture, which are vascular endothelial cell-markers. Also, VEGF-receptor type-1, and -2, and VE-cadherin expressed significantly. Morphologically, HDF was changed to an endothelial cell. When HDF was further cultured, blast cells were attached to an endothelial cell, and SCL, and GATA-2 molecule expressed significantly.
Conclusion
Recent reports reveal that a kind of vascular endothelial cells, hemogenic endothelium, can be converted into a hematopoietic cell. We identified that IL-1-b worked an important role in vascular endothelial cell-formation.
Session topic: E-poster
Keyword(s): CD34, Vasculogenesis
Type: Eposter Presentation
Background
We previously reported that when an adult human dermal fibroblast (HDF) was cultured with interleukin (IL)-1-beta (b), vascular endothelial growth factor (VEGF)-A, erythropoietin (EPO), anti-human VEGF-C antibody (Ab) and anti-human tumor necrosis factor (TNF)-alpha (a) Ab, hematopoiesis-related genes expressed; however, culturing cells were morphologically fibroblasts (56th ASH). We also reported that hemogenic endothelium-relating molecules expressed in HDF with poly (I:C) culture and an electrical stimulation (44th ISEH).
Aims
In this report we observed the biological effects of IL-1-b to HDF for up to one month-culture.
Methods
HDF was cultured with CELRENA medium, used for induced pluripotent stem (iPS) cell-culture, on 0.1 % gelatin-coated plate (porcine skin). Cultures were added with recombinant human IL-1-b for up to four weeks with splitting each one week. Morphological changes and the expression of vascular endothelial cell-related genes, and hematopoiesis-related ones were observed.
Results
When HDF was cultured with IL-1-b, CD31, CD34, and CD41 molecules expressed for two weeks’ culture, which are vascular endothelial cell-markers. Also, VEGF-receptor type-1, and -2, and VE-cadherin expressed significantly. Morphologically, HDF was changed to an endothelial cell. When HDF was further cultured, blast cells were attached to an endothelial cell, and SCL, and GATA-2 molecule expressed significantly.
Conclusion
Recent reports reveal that a kind of vascular endothelial cells, hemogenic endothelium, can be converted into a hematopoietic cell. We identified that IL-1-b worked an important role in vascular endothelial cell-formation.
Session topic: E-poster
Keyword(s): CD34, Vasculogenesis
Abstract: E1139
Type: Eposter Presentation
Background
We previously reported that when an adult human dermal fibroblast (HDF) was cultured with interleukin (IL)-1-beta (b), vascular endothelial growth factor (VEGF)-A, erythropoietin (EPO), anti-human VEGF-C antibody (Ab) and anti-human tumor necrosis factor (TNF)-alpha (a) Ab, hematopoiesis-related genes expressed; however, culturing cells were morphologically fibroblasts (56th ASH). We also reported that hemogenic endothelium-relating molecules expressed in HDF with poly (I:C) culture and an electrical stimulation (44th ISEH).
Aims
In this report we observed the biological effects of IL-1-b to HDF for up to one month-culture.
Methods
HDF was cultured with CELRENA medium, used for induced pluripotent stem (iPS) cell-culture, on 0.1 % gelatin-coated plate (porcine skin). Cultures were added with recombinant human IL-1-b for up to four weeks with splitting each one week. Morphological changes and the expression of vascular endothelial cell-related genes, and hematopoiesis-related ones were observed.
Results
When HDF was cultured with IL-1-b, CD31, CD34, and CD41 molecules expressed for two weeks’ culture, which are vascular endothelial cell-markers. Also, VEGF-receptor type-1, and -2, and VE-cadherin expressed significantly. Morphologically, HDF was changed to an endothelial cell. When HDF was further cultured, blast cells were attached to an endothelial cell, and SCL, and GATA-2 molecule expressed significantly.
Conclusion
Recent reports reveal that a kind of vascular endothelial cells, hemogenic endothelium, can be converted into a hematopoietic cell. We identified that IL-1-b worked an important role in vascular endothelial cell-formation.
Session topic: E-poster
Keyword(s): CD34, Vasculogenesis
Type: Eposter Presentation
Background
We previously reported that when an adult human dermal fibroblast (HDF) was cultured with interleukin (IL)-1-beta (b), vascular endothelial growth factor (VEGF)-A, erythropoietin (EPO), anti-human VEGF-C antibody (Ab) and anti-human tumor necrosis factor (TNF)-alpha (a) Ab, hematopoiesis-related genes expressed; however, culturing cells were morphologically fibroblasts (56th ASH). We also reported that hemogenic endothelium-relating molecules expressed in HDF with poly (I:C) culture and an electrical stimulation (44th ISEH).
Aims
In this report we observed the biological effects of IL-1-b to HDF for up to one month-culture.
Methods
HDF was cultured with CELRENA medium, used for induced pluripotent stem (iPS) cell-culture, on 0.1 % gelatin-coated plate (porcine skin). Cultures were added with recombinant human IL-1-b for up to four weeks with splitting each one week. Morphological changes and the expression of vascular endothelial cell-related genes, and hematopoiesis-related ones were observed.
Results
When HDF was cultured with IL-1-b, CD31, CD34, and CD41 molecules expressed for two weeks’ culture, which are vascular endothelial cell-markers. Also, VEGF-receptor type-1, and -2, and VE-cadherin expressed significantly. Morphologically, HDF was changed to an endothelial cell. When HDF was further cultured, blast cells were attached to an endothelial cell, and SCL, and GATA-2 molecule expressed significantly.
Conclusion
Recent reports reveal that a kind of vascular endothelial cells, hemogenic endothelium, can be converted into a hematopoietic cell. We identified that IL-1-b worked an important role in vascular endothelial cell-formation.
Session topic: E-poster
Keyword(s): CD34, Vasculogenesis
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