THE EFFECTS OF VARIOUS BLOOD PROTEINS ON THE ZETA POTENTIAL OF POLYMER NANOPARTICLES AND PROTEIN CORONA FORMATION: AN ANALYSIS BY TUNABLE RESISTIVE PULSE SENSING
(Abstract release date: 05/19/16)
EHA Library. Sivakumaran M. 06/09/16; 132672; E1123
Dr. M Sivakumaran
Contributions
Contributions
Abstract
Abstract: E1123
Type: Eposter Presentation
Background
Nanoparticles are increasingly used as delivery vehicles for targeted drug delivery, gene delivery, imaging and theranostics. Upon introduction of nanoparticles into biological fluids such as blood, the particles interact with the constituents of the environment, in particular with proteins. The interaction of nanoparticles with the proteins leads to formation of a protein-corona and change in the surface charge of the particles. These changes significantly affect the pharmacokinetic, pharmacodynamic and toxicological properties of the nanoparticles.Tunable Resistive Pulse Sensing (TRPS) technology, based on Coulter principle, enables particle-by-particle measurement of the size and zeta potential of nanoparticles during their passage through a tunable pore (Langmuir (2016) 32,1082-1090.
Aims
To ascertain the effects of three predominant proteins in blood, namely albumin, immunoglobulin and fibrinogen, in biologically relevant concentrations on the zeta potential of carboxylated polymer nanoparticles (CPNPs) in saline, serum and plasma at room temperature and 37oC using TRPS
Methods
The particle size and zeta potential of carboxylated polymer nanoparticles were studied in phosphate buffered saline, albumin (40g/l), immunoglobulin (20g/l), fibrinogen (4g/l), normal human serum and normal human plasma at room temperature and 37oC using TRPS (qNano, Izon Sciences, Oxford, UK). The kinetics of the protein corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma was then studied by monitoring changes in size and zeta potential of the particles using qNano.
Results
We observed a significant difference in distributions and zeta values between room temperature and 37oC assay. The effect was protein dependent, and the largest difference between the two temperatures was recorded for the γ-globulin protein where the mean zeta potential changed from -16.7 mV to -9.0 mV for 25 and 37oC, respectively. This method was further applied to monitor particles placed into serum and/or plasma. A temperature dependent change was again observed with serum showing a 4.9 mV difference in zeta potential between samples incubated at 25oC and 37oC, this shift was larger than that observed for samples in plasma (0.4 mV). Finally we monitored the kinetics of the corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma.
Conclusion
The technology presented offers an interesting insight into protein corona structure and kinetics of formation measured in biologically relevant solutions i.e. high protein, high salt levels and its particle-by-particle analysis gives a measure of the distribution of particle zeta potential that may offer a better understanding of the behaviour of nanoparticles used for drug/gene delivery in blood.
Session topic: E-poster
Keyword(s): Gene therapy, Nanoparticle, Plasma
Type: Eposter Presentation
Background
Nanoparticles are increasingly used as delivery vehicles for targeted drug delivery, gene delivery, imaging and theranostics. Upon introduction of nanoparticles into biological fluids such as blood, the particles interact with the constituents of the environment, in particular with proteins. The interaction of nanoparticles with the proteins leads to formation of a protein-corona and change in the surface charge of the particles. These changes significantly affect the pharmacokinetic, pharmacodynamic and toxicological properties of the nanoparticles.Tunable Resistive Pulse Sensing (TRPS) technology, based on Coulter principle, enables particle-by-particle measurement of the size and zeta potential of nanoparticles during their passage through a tunable pore (Langmuir (2016) 32,1082-1090.
Aims
To ascertain the effects of three predominant proteins in blood, namely albumin, immunoglobulin and fibrinogen, in biologically relevant concentrations on the zeta potential of carboxylated polymer nanoparticles (CPNPs) in saline, serum and plasma at room temperature and 37oC using TRPS
Methods
The particle size and zeta potential of carboxylated polymer nanoparticles were studied in phosphate buffered saline, albumin (40g/l), immunoglobulin (20g/l), fibrinogen (4g/l), normal human serum and normal human plasma at room temperature and 37oC using TRPS (qNano, Izon Sciences, Oxford, UK). The kinetics of the protein corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma was then studied by monitoring changes in size and zeta potential of the particles using qNano.
Results
We observed a significant difference in distributions and zeta values between room temperature and 37oC assay. The effect was protein dependent, and the largest difference between the two temperatures was recorded for the γ-globulin protein where the mean zeta potential changed from -16.7 mV to -9.0 mV for 25 and 37oC, respectively. This method was further applied to monitor particles placed into serum and/or plasma. A temperature dependent change was again observed with serum showing a 4.9 mV difference in zeta potential between samples incubated at 25oC and 37oC, this shift was larger than that observed for samples in plasma (0.4 mV). Finally we monitored the kinetics of the corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma.
Conclusion
The technology presented offers an interesting insight into protein corona structure and kinetics of formation measured in biologically relevant solutions i.e. high protein, high salt levels and its particle-by-particle analysis gives a measure of the distribution of particle zeta potential that may offer a better understanding of the behaviour of nanoparticles used for drug/gene delivery in blood.
Session topic: E-poster
Keyword(s): Gene therapy, Nanoparticle, Plasma
Abstract: E1123
Type: Eposter Presentation
Background
Nanoparticles are increasingly used as delivery vehicles for targeted drug delivery, gene delivery, imaging and theranostics. Upon introduction of nanoparticles into biological fluids such as blood, the particles interact with the constituents of the environment, in particular with proteins. The interaction of nanoparticles with the proteins leads to formation of a protein-corona and change in the surface charge of the particles. These changes significantly affect the pharmacokinetic, pharmacodynamic and toxicological properties of the nanoparticles.Tunable Resistive Pulse Sensing (TRPS) technology, based on Coulter principle, enables particle-by-particle measurement of the size and zeta potential of nanoparticles during their passage through a tunable pore (Langmuir (2016) 32,1082-1090.
Aims
To ascertain the effects of three predominant proteins in blood, namely albumin, immunoglobulin and fibrinogen, in biologically relevant concentrations on the zeta potential of carboxylated polymer nanoparticles (CPNPs) in saline, serum and plasma at room temperature and 37oC using TRPS
Methods
The particle size and zeta potential of carboxylated polymer nanoparticles were studied in phosphate buffered saline, albumin (40g/l), immunoglobulin (20g/l), fibrinogen (4g/l), normal human serum and normal human plasma at room temperature and 37oC using TRPS (qNano, Izon Sciences, Oxford, UK). The kinetics of the protein corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma was then studied by monitoring changes in size and zeta potential of the particles using qNano.
Results
We observed a significant difference in distributions and zeta values between room temperature and 37oC assay. The effect was protein dependent, and the largest difference between the two temperatures was recorded for the γ-globulin protein where the mean zeta potential changed from -16.7 mV to -9.0 mV for 25 and 37oC, respectively. This method was further applied to monitor particles placed into serum and/or plasma. A temperature dependent change was again observed with serum showing a 4.9 mV difference in zeta potential between samples incubated at 25oC and 37oC, this shift was larger than that observed for samples in plasma (0.4 mV). Finally we monitored the kinetics of the corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma.
Conclusion
The technology presented offers an interesting insight into protein corona structure and kinetics of formation measured in biologically relevant solutions i.e. high protein, high salt levels and its particle-by-particle analysis gives a measure of the distribution of particle zeta potential that may offer a better understanding of the behaviour of nanoparticles used for drug/gene delivery in blood.
Session topic: E-poster
Keyword(s): Gene therapy, Nanoparticle, Plasma
Type: Eposter Presentation
Background
Nanoparticles are increasingly used as delivery vehicles for targeted drug delivery, gene delivery, imaging and theranostics. Upon introduction of nanoparticles into biological fluids such as blood, the particles interact with the constituents of the environment, in particular with proteins. The interaction of nanoparticles with the proteins leads to formation of a protein-corona and change in the surface charge of the particles. These changes significantly affect the pharmacokinetic, pharmacodynamic and toxicological properties of the nanoparticles.Tunable Resistive Pulse Sensing (TRPS) technology, based on Coulter principle, enables particle-by-particle measurement of the size and zeta potential of nanoparticles during their passage through a tunable pore (Langmuir (2016) 32,1082-1090.
Aims
To ascertain the effects of three predominant proteins in blood, namely albumin, immunoglobulin and fibrinogen, in biologically relevant concentrations on the zeta potential of carboxylated polymer nanoparticles (CPNPs) in saline, serum and plasma at room temperature and 37oC using TRPS
Methods
The particle size and zeta potential of carboxylated polymer nanoparticles were studied in phosphate buffered saline, albumin (40g/l), immunoglobulin (20g/l), fibrinogen (4g/l), normal human serum and normal human plasma at room temperature and 37oC using TRPS (qNano, Izon Sciences, Oxford, UK). The kinetics of the protein corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma was then studied by monitoring changes in size and zeta potential of the particles using qNano.
Results
We observed a significant difference in distributions and zeta values between room temperature and 37oC assay. The effect was protein dependent, and the largest difference between the two temperatures was recorded for the γ-globulin protein where the mean zeta potential changed from -16.7 mV to -9.0 mV for 25 and 37oC, respectively. This method was further applied to monitor particles placed into serum and/or plasma. A temperature dependent change was again observed with serum showing a 4.9 mV difference in zeta potential between samples incubated at 25oC and 37oC, this shift was larger than that observed for samples in plasma (0.4 mV). Finally we monitored the kinetics of the corona reorientation for particles initially placed into serum and then adding 5%(V/V) plasma.
Conclusion
The technology presented offers an interesting insight into protein corona structure and kinetics of formation measured in biologically relevant solutions i.e. high protein, high salt levels and its particle-by-particle analysis gives a measure of the distribution of particle zeta potential that may offer a better understanding of the behaviour of nanoparticles used for drug/gene delivery in blood.
Session topic: E-poster
Keyword(s): Gene therapy, Nanoparticle, Plasma
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