PREVALANCE OF CHROMOSALLY-INTEGRATED HERPESVIRUS 6 IN PATIENTS WITH APLASTIC ANEMIA.
(Abstract release date: 05/19/16)
EHA Library. Bakker M. 06/09/16; 132555; E1006
Mr. Martijn Bakker
Contributions
Contributions
Abstract
Abstract: E1006
Type: Eposter Presentation
Background
Aplastic anemia (AA) is a rare heterogeneous bone marrow failure syndrome. It is hypothesized that in most cases AA is an immune-mediated disease. One third of patients, especially those who do not respond to immunosuppressive therapy, have shortening of telomeres in there leucocytes. Mutations in genes responsible for telomere repair proteins are present in some of these patients. Human herpesvirus 6 is one of the herpesviruses which establish latent infection. It is shown that reactivation of latent HHV-6 can have direct suppressive effects on hematopoietic progenitors which can be the cause of bone marrow suppression after hematopoietic stemcell transplantation. Besides latent infection, HHV-6 can integrate in the human DNA at the telomeric region which results in germ-line transmission of the HHV-6 genome. Chromosomally integrated HHV-6 (ciHHV-6) can be found in less than 1% of the population.
Aims
We performed an exploratory study to investigate the prevalence of ciHHV-6 in patients with AA in order to determine whether ciHHV-6 represents a risk factor for developing AA.
Methods
Between 2008 and 2015 101 patients were treated for AA at the University Medical Center Groningen in The Netherlands. Whole blood samples of 53 of these patients that were initially used for clinical purposes were available for analysis by quantitative PCR (qPCR) for the presence of HHV-6 DNA.
Results
Of the 53 samples 11 patients had detectable HHV-6 DNA. Ten patients had a low HHV-6 viral load with median concentration of 1.9 x102 copies/ml (range 1.2 x106-2.3 x 104 copies/ml). One patient had a high viral load with 5.2 x106 copies/ml suggestive of ciHHV-6. Viral chromosomal integration was subsequently proved by demonstrating HHV-6 DNA in hair follicles of this patient. In our cohort the prevalence of ciHHV-6 was 1.9%, which is not above the expected incidence of the general population.
Conclusion
The prevalence of ciHHV-6 in patients with AA is similar to that of the general population. It is therefore unlikely that ciHHV-6 is a risk factor for AA.
Session topic: E-poster
Keyword(s): Aplastic anemia, Herpesvirus, Telomere
Type: Eposter Presentation
Background
Aplastic anemia (AA) is a rare heterogeneous bone marrow failure syndrome. It is hypothesized that in most cases AA is an immune-mediated disease. One third of patients, especially those who do not respond to immunosuppressive therapy, have shortening of telomeres in there leucocytes. Mutations in genes responsible for telomere repair proteins are present in some of these patients. Human herpesvirus 6 is one of the herpesviruses which establish latent infection. It is shown that reactivation of latent HHV-6 can have direct suppressive effects on hematopoietic progenitors which can be the cause of bone marrow suppression after hematopoietic stemcell transplantation. Besides latent infection, HHV-6 can integrate in the human DNA at the telomeric region which results in germ-line transmission of the HHV-6 genome. Chromosomally integrated HHV-6 (ciHHV-6) can be found in less than 1% of the population.
Aims
We performed an exploratory study to investigate the prevalence of ciHHV-6 in patients with AA in order to determine whether ciHHV-6 represents a risk factor for developing AA.
Methods
Between 2008 and 2015 101 patients were treated for AA at the University Medical Center Groningen in The Netherlands. Whole blood samples of 53 of these patients that were initially used for clinical purposes were available for analysis by quantitative PCR (qPCR) for the presence of HHV-6 DNA.
Results
Of the 53 samples 11 patients had detectable HHV-6 DNA. Ten patients had a low HHV-6 viral load with median concentration of 1.9 x102 copies/ml (range 1.2 x106-2.3 x 104 copies/ml). One patient had a high viral load with 5.2 x106 copies/ml suggestive of ciHHV-6. Viral chromosomal integration was subsequently proved by demonstrating HHV-6 DNA in hair follicles of this patient. In our cohort the prevalence of ciHHV-6 was 1.9%, which is not above the expected incidence of the general population.
Conclusion
The prevalence of ciHHV-6 in patients with AA is similar to that of the general population. It is therefore unlikely that ciHHV-6 is a risk factor for AA.
Session topic: E-poster
Keyword(s): Aplastic anemia, Herpesvirus, Telomere
Abstract: E1006
Type: Eposter Presentation
Background
Aplastic anemia (AA) is a rare heterogeneous bone marrow failure syndrome. It is hypothesized that in most cases AA is an immune-mediated disease. One third of patients, especially those who do not respond to immunosuppressive therapy, have shortening of telomeres in there leucocytes. Mutations in genes responsible for telomere repair proteins are present in some of these patients. Human herpesvirus 6 is one of the herpesviruses which establish latent infection. It is shown that reactivation of latent HHV-6 can have direct suppressive effects on hematopoietic progenitors which can be the cause of bone marrow suppression after hematopoietic stemcell transplantation. Besides latent infection, HHV-6 can integrate in the human DNA at the telomeric region which results in germ-line transmission of the HHV-6 genome. Chromosomally integrated HHV-6 (ciHHV-6) can be found in less than 1% of the population.
Aims
We performed an exploratory study to investigate the prevalence of ciHHV-6 in patients with AA in order to determine whether ciHHV-6 represents a risk factor for developing AA.
Methods
Between 2008 and 2015 101 patients were treated for AA at the University Medical Center Groningen in The Netherlands. Whole blood samples of 53 of these patients that were initially used for clinical purposes were available for analysis by quantitative PCR (qPCR) for the presence of HHV-6 DNA.
Results
Of the 53 samples 11 patients had detectable HHV-6 DNA. Ten patients had a low HHV-6 viral load with median concentration of 1.9 x102 copies/ml (range 1.2 x106-2.3 x 104 copies/ml). One patient had a high viral load with 5.2 x106 copies/ml suggestive of ciHHV-6. Viral chromosomal integration was subsequently proved by demonstrating HHV-6 DNA in hair follicles of this patient. In our cohort the prevalence of ciHHV-6 was 1.9%, which is not above the expected incidence of the general population.
Conclusion
The prevalence of ciHHV-6 in patients with AA is similar to that of the general population. It is therefore unlikely that ciHHV-6 is a risk factor for AA.
Session topic: E-poster
Keyword(s): Aplastic anemia, Herpesvirus, Telomere
Type: Eposter Presentation
Background
Aplastic anemia (AA) is a rare heterogeneous bone marrow failure syndrome. It is hypothesized that in most cases AA is an immune-mediated disease. One third of patients, especially those who do not respond to immunosuppressive therapy, have shortening of telomeres in there leucocytes. Mutations in genes responsible for telomere repair proteins are present in some of these patients. Human herpesvirus 6 is one of the herpesviruses which establish latent infection. It is shown that reactivation of latent HHV-6 can have direct suppressive effects on hematopoietic progenitors which can be the cause of bone marrow suppression after hematopoietic stemcell transplantation. Besides latent infection, HHV-6 can integrate in the human DNA at the telomeric region which results in germ-line transmission of the HHV-6 genome. Chromosomally integrated HHV-6 (ciHHV-6) can be found in less than 1% of the population.
Aims
We performed an exploratory study to investigate the prevalence of ciHHV-6 in patients with AA in order to determine whether ciHHV-6 represents a risk factor for developing AA.
Methods
Between 2008 and 2015 101 patients were treated for AA at the University Medical Center Groningen in The Netherlands. Whole blood samples of 53 of these patients that were initially used for clinical purposes were available for analysis by quantitative PCR (qPCR) for the presence of HHV-6 DNA.
Results
Of the 53 samples 11 patients had detectable HHV-6 DNA. Ten patients had a low HHV-6 viral load with median concentration of 1.9 x102 copies/ml (range 1.2 x106-2.3 x 104 copies/ml). One patient had a high viral load with 5.2 x106 copies/ml suggestive of ciHHV-6. Viral chromosomal integration was subsequently proved by demonstrating HHV-6 DNA in hair follicles of this patient. In our cohort the prevalence of ciHHV-6 was 1.9%, which is not above the expected incidence of the general population.
Conclusion
The prevalence of ciHHV-6 in patients with AA is similar to that of the general population. It is therefore unlikely that ciHHV-6 is a risk factor for AA.
Session topic: E-poster
Keyword(s): Aplastic anemia, Herpesvirus, Telomere
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