SERUM CYTOKINE AND CHEMOKINE LEVELS IN CHILDREN WITH IMMUNE THROMBOCYTOPENIA
(Abstract release date: 05/19/16)
EHA Library. KOMITOPOULOU A. 06/09/16; 132553; E1004
Disclosure(s): No disclosure
Dr. Anna KOMITOPOULOU
Contributions
Contributions
Abstract
Abstract: E1004
Type: Eposter Presentation
Background
Immune thrombocytopenia (ITP) is an acquired immune disorder derived from disruption of platelet production and removal from blood circulation, usually as a consequence of autoantibody and cytotoxic T-lymphocyte development against them, which ultimately results to the phagocytosis of the trapped platelets from monocytes. Onset of ITP in children is often triggered by viral infections, infectious diseases or vaccination. In pediatric patients with ITP, as in adults, impaired immunity by deregulation of the balance of Th1/Th2 (T-helper) and of Tregs (regulatory T cells) is referred. The cytokines which are secreted by activated lymphocytes control the immune system either by stimulating or inhibiting the activity of cells but simultaneously they are involved in the production of antiplatelet antibodies or other cytokines and/or chemokines.
Aims
The aim of our study was to determine the serum levels of specific Th cytokines and chemokines in children with ITP and to correlate them with clinical parameters.
Methods
The group of patients consisted of 18 children with ITP (8 male,10 female) of a mean age at investigation 5.46 years. ITP was chronic in 6/18 children, while acute/persistent was in 12/18 (duration of thrombocytopenia>12 or <12 months, respectively). None but one patient had received any treatment during the last 3 months. The control group was constituted by 37 children of preschool age. Serum levels of cytokines IL-1a, TNF-a, IL-1b, IL-10, IL-12p40, hsIL-6 and chemokines of macrophages such as IL-8 (CXCL8) and MCP-1 (CCL2) were measured in patients and controls using the cytokine-chemokine- MAP technology which is a bead based multiplex assay using the Luminex. Inform consent was obtained from the parents of both groups.
Results
There was no significant difference in the concentrations of IL-1a, Il-1b, IL-10, IL12p40 and the chemokine IL-8 among the two groups, except of the concentration of TNF-a which was found quite lower among patients and controls. Furthermore, only the levels of TNF-a differed remarkably between children with acute/persistent compared to children with chronic ITP (p<0.05). A significant reduction of hsIL-6 (1.0±0.2 vs 1.6±0.2pg/mL, p<0.05) and MCP-1 (252.7±20.0 vs 408.5±22.2pg/mL, p<0.001) was noticed in children with ITP.
Conclusion
The current study did not seem to confirm the hypothesis of the overall increase of proinflammatory cytokines in children with ITP. The higher values of TNF-a in the initial phase of the disease could be interpreted as a response to inflammation. The finding of the dramatically reduced chemotactic protein MCP-1 (CCL2), possibly reveals deficient response in the ability to control inflammation, a conclusion reinforced by a recent study in murine models of human ITP1. The exact role of various cytokines/chemokines in the deregulation of the immune system, sush as occurs in autoimmune diseases, remains to be investigated, particularly in children which are often on inflammatory process. Well-designed studies with a larger number of children with ITP and larger subsets of patients at different stages of the disease, as well, which will investigate a wider range of cytokines, may provide useful answers in the future. 1Leontyev et al. Transfusion, 2014; 54:2871.
Session topic: E-poster
Keyword(s): Chemokine, Children, Cytokine, Immune thrombocytopenia (ITP)
Type: Eposter Presentation
Background
Immune thrombocytopenia (ITP) is an acquired immune disorder derived from disruption of platelet production and removal from blood circulation, usually as a consequence of autoantibody and cytotoxic T-lymphocyte development against them, which ultimately results to the phagocytosis of the trapped platelets from monocytes. Onset of ITP in children is often triggered by viral infections, infectious diseases or vaccination. In pediatric patients with ITP, as in adults, impaired immunity by deregulation of the balance of Th1/Th2 (T-helper) and of Tregs (regulatory T cells) is referred. The cytokines which are secreted by activated lymphocytes control the immune system either by stimulating or inhibiting the activity of cells but simultaneously they are involved in the production of antiplatelet antibodies or other cytokines and/or chemokines.
Aims
The aim of our study was to determine the serum levels of specific Th cytokines and chemokines in children with ITP and to correlate them with clinical parameters.
Methods
The group of patients consisted of 18 children with ITP (8 male,10 female) of a mean age at investigation 5.46 years. ITP was chronic in 6/18 children, while acute/persistent was in 12/18 (duration of thrombocytopenia>12 or <12 months, respectively). None but one patient had received any treatment during the last 3 months. The control group was constituted by 37 children of preschool age. Serum levels of cytokines IL-1a, TNF-a, IL-1b, IL-10, IL-12p40, hsIL-6 and chemokines of macrophages such as IL-8 (CXCL8) and MCP-1 (CCL2) were measured in patients and controls using the cytokine-chemokine- MAP technology which is a bead based multiplex assay using the Luminex. Inform consent was obtained from the parents of both groups.
Results
There was no significant difference in the concentrations of IL-1a, Il-1b, IL-10, IL12p40 and the chemokine IL-8 among the two groups, except of the concentration of TNF-a which was found quite lower among patients and controls. Furthermore, only the levels of TNF-a differed remarkably between children with acute/persistent compared to children with chronic ITP (p<0.05). A significant reduction of hsIL-6 (1.0±0.2 vs 1.6±0.2pg/mL, p<0.05) and MCP-1 (252.7±20.0 vs 408.5±22.2pg/mL, p<0.001) was noticed in children with ITP.
Conclusion
The current study did not seem to confirm the hypothesis of the overall increase of proinflammatory cytokines in children with ITP. The higher values of TNF-a in the initial phase of the disease could be interpreted as a response to inflammation. The finding of the dramatically reduced chemotactic protein MCP-1 (CCL2), possibly reveals deficient response in the ability to control inflammation, a conclusion reinforced by a recent study in murine models of human ITP1. The exact role of various cytokines/chemokines in the deregulation of the immune system, sush as occurs in autoimmune diseases, remains to be investigated, particularly in children which are often on inflammatory process. Well-designed studies with a larger number of children with ITP and larger subsets of patients at different stages of the disease, as well, which will investigate a wider range of cytokines, may provide useful answers in the future. 1Leontyev et al. Transfusion, 2014; 54:2871.
Session topic: E-poster
Keyword(s): Chemokine, Children, Cytokine, Immune thrombocytopenia (ITP)
Abstract: E1004
Type: Eposter Presentation
Background
Immune thrombocytopenia (ITP) is an acquired immune disorder derived from disruption of platelet production and removal from blood circulation, usually as a consequence of autoantibody and cytotoxic T-lymphocyte development against them, which ultimately results to the phagocytosis of the trapped platelets from monocytes. Onset of ITP in children is often triggered by viral infections, infectious diseases or vaccination. In pediatric patients with ITP, as in adults, impaired immunity by deregulation of the balance of Th1/Th2 (T-helper) and of Tregs (regulatory T cells) is referred. The cytokines which are secreted by activated lymphocytes control the immune system either by stimulating or inhibiting the activity of cells but simultaneously they are involved in the production of antiplatelet antibodies or other cytokines and/or chemokines.
Aims
The aim of our study was to determine the serum levels of specific Th cytokines and chemokines in children with ITP and to correlate them with clinical parameters.
Methods
The group of patients consisted of 18 children with ITP (8 male,10 female) of a mean age at investigation 5.46 years. ITP was chronic in 6/18 children, while acute/persistent was in 12/18 (duration of thrombocytopenia>12 or <12 months, respectively). None but one patient had received any treatment during the last 3 months. The control group was constituted by 37 children of preschool age. Serum levels of cytokines IL-1a, TNF-a, IL-1b, IL-10, IL-12p40, hsIL-6 and chemokines of macrophages such as IL-8 (CXCL8) and MCP-1 (CCL2) were measured in patients and controls using the cytokine-chemokine- MAP technology which is a bead based multiplex assay using the Luminex. Inform consent was obtained from the parents of both groups.
Results
There was no significant difference in the concentrations of IL-1a, Il-1b, IL-10, IL12p40 and the chemokine IL-8 among the two groups, except of the concentration of TNF-a which was found quite lower among patients and controls. Furthermore, only the levels of TNF-a differed remarkably between children with acute/persistent compared to children with chronic ITP (p<0.05). A significant reduction of hsIL-6 (1.0±0.2 vs 1.6±0.2pg/mL, p<0.05) and MCP-1 (252.7±20.0 vs 408.5±22.2pg/mL, p<0.001) was noticed in children with ITP.
Conclusion
The current study did not seem to confirm the hypothesis of the overall increase of proinflammatory cytokines in children with ITP. The higher values of TNF-a in the initial phase of the disease could be interpreted as a response to inflammation. The finding of the dramatically reduced chemotactic protein MCP-1 (CCL2), possibly reveals deficient response in the ability to control inflammation, a conclusion reinforced by a recent study in murine models of human ITP1. The exact role of various cytokines/chemokines in the deregulation of the immune system, sush as occurs in autoimmune diseases, remains to be investigated, particularly in children which are often on inflammatory process. Well-designed studies with a larger number of children with ITP and larger subsets of patients at different stages of the disease, as well, which will investigate a wider range of cytokines, may provide useful answers in the future. 1Leontyev et al. Transfusion, 2014; 54:2871.
Session topic: E-poster
Keyword(s): Chemokine, Children, Cytokine, Immune thrombocytopenia (ITP)
Type: Eposter Presentation
Background
Immune thrombocytopenia (ITP) is an acquired immune disorder derived from disruption of platelet production and removal from blood circulation, usually as a consequence of autoantibody and cytotoxic T-lymphocyte development against them, which ultimately results to the phagocytosis of the trapped platelets from monocytes. Onset of ITP in children is often triggered by viral infections, infectious diseases or vaccination. In pediatric patients with ITP, as in adults, impaired immunity by deregulation of the balance of Th1/Th2 (T-helper) and of Tregs (regulatory T cells) is referred. The cytokines which are secreted by activated lymphocytes control the immune system either by stimulating or inhibiting the activity of cells but simultaneously they are involved in the production of antiplatelet antibodies or other cytokines and/or chemokines.
Aims
The aim of our study was to determine the serum levels of specific Th cytokines and chemokines in children with ITP and to correlate them with clinical parameters.
Methods
The group of patients consisted of 18 children with ITP (8 male,10 female) of a mean age at investigation 5.46 years. ITP was chronic in 6/18 children, while acute/persistent was in 12/18 (duration of thrombocytopenia>12 or <12 months, respectively). None but one patient had received any treatment during the last 3 months. The control group was constituted by 37 children of preschool age. Serum levels of cytokines IL-1a, TNF-a, IL-1b, IL-10, IL-12p40, hsIL-6 and chemokines of macrophages such as IL-8 (CXCL8) and MCP-1 (CCL2) were measured in patients and controls using the cytokine-chemokine- MAP technology which is a bead based multiplex assay using the Luminex. Inform consent was obtained from the parents of both groups.
Results
There was no significant difference in the concentrations of IL-1a, Il-1b, IL-10, IL12p40 and the chemokine IL-8 among the two groups, except of the concentration of TNF-a which was found quite lower among patients and controls. Furthermore, only the levels of TNF-a differed remarkably between children with acute/persistent compared to children with chronic ITP (p<0.05). A significant reduction of hsIL-6 (1.0±0.2 vs 1.6±0.2pg/mL, p<0.05) and MCP-1 (252.7±20.0 vs 408.5±22.2pg/mL, p<0.001) was noticed in children with ITP.
Conclusion
The current study did not seem to confirm the hypothesis of the overall increase of proinflammatory cytokines in children with ITP. The higher values of TNF-a in the initial phase of the disease could be interpreted as a response to inflammation. The finding of the dramatically reduced chemotactic protein MCP-1 (CCL2), possibly reveals deficient response in the ability to control inflammation, a conclusion reinforced by a recent study in murine models of human ITP1. The exact role of various cytokines/chemokines in the deregulation of the immune system, sush as occurs in autoimmune diseases, remains to be investigated, particularly in children which are often on inflammatory process. Well-designed studies with a larger number of children with ITP and larger subsets of patients at different stages of the disease, as well, which will investigate a wider range of cytokines, may provide useful answers in the future. 1Leontyev et al. Transfusion, 2014; 54:2871.
Session topic: E-poster
Keyword(s): Chemokine, Children, Cytokine, Immune thrombocytopenia (ITP)
{{ help_message }}
{{filter}}