IS CD45 EXPRESSION LEVEL RELATED TO HIGH RISK DEFINITION IN CHILDHOOD PEDIATRIC B ALL ?
(Abstract release date: 05/21/15)
EHA Library. Albayrak D. 06/12/15; 103006; PB1619
Disclosure(s): Samsun Ondokuz Mayis University Faculty Of MedicinePediatric Hematology Department
Davut Albayrak
Contributions
Contributions
Abstract
Abstract: PB1619
Type: Publication Only
Background
Two report shows CD45 brightnes of childhood ALL blast is associated with poor prognosis. Borowitz et al showed that bright CD45 expression was associated with a poor prognosis in BCP-ALL patients treated according to theprotocols of the Pediatric Oncology Groupstudy group and Cario et al also in ALL-BFM-2000 protocol.
Aims
In the present study, we assessed the relation of brightness of CD45 expression in pediatric ALL patients treated according to he ALL-IC-BFM 2009 protocol.
Methods
The brightness of CD45 expression was compared in ten patients of high risk group and ten patient of standart and intermediate groups. In the ALL-IC BFM 2009 trial, the high-risk group was defined by poor prednisone response on treatment day 8, FCM blast ratio >%10 at day 15 and BCR-ABL OR MLL positivity. The brightness of CD45 expression was evaluated as mean flouresence index (MFI).
Results
CD45 MFI was 710,2 for blast, 13784,6 for normal lymphocytes, 0,056 for blast/lymphocytes in hr group, CD45 MFI was 590 for blast, 7904,4 for normal lymphocytes, 0,082 fr blast/lymphocytes in hr group. The differenceb between groups was not significantly different.
Summary
In conclusion, the CD45 brightnes of childhood BCP-ALL did nod have any additional contribution to high risk stratification. This result is different from previous reports. New reports is required to solve this debate.
Session topic: Publication Only
Type: Publication Only
Background
Two report shows CD45 brightnes of childhood ALL blast is associated with poor prognosis. Borowitz et al showed that bright CD45 expression was associated with a poor prognosis in BCP-ALL patients treated according to theprotocols of the Pediatric Oncology Groupstudy group and Cario et al also in ALL-BFM-2000 protocol.
Aims
In the present study, we assessed the relation of brightness of CD45 expression in pediatric ALL patients treated according to he ALL-IC-BFM 2009 protocol.
Methods
The brightness of CD45 expression was compared in ten patients of high risk group and ten patient of standart and intermediate groups. In the ALL-IC BFM 2009 trial, the high-risk group was defined by poor prednisone response on treatment day 8, FCM blast ratio >%10 at day 15 and BCR-ABL OR MLL positivity. The brightness of CD45 expression was evaluated as mean flouresence index (MFI).
Results
CD45 MFI was 710,2 for blast, 13784,6 for normal lymphocytes, 0,056 for blast/lymphocytes in hr group, CD45 MFI was 590 for blast, 7904,4 for normal lymphocytes, 0,082 fr blast/lymphocytes in hr group. The differenceb between groups was not significantly different.
Summary
In conclusion, the CD45 brightnes of childhood BCP-ALL did nod have any additional contribution to high risk stratification. This result is different from previous reports. New reports is required to solve this debate.
Session topic: Publication Only
Abstract: PB1619
Type: Publication Only
Background
Two report shows CD45 brightnes of childhood ALL blast is associated with poor prognosis. Borowitz et al showed that bright CD45 expression was associated with a poor prognosis in BCP-ALL patients treated according to theprotocols of the Pediatric Oncology Groupstudy group and Cario et al also in ALL-BFM-2000 protocol.
Aims
In the present study, we assessed the relation of brightness of CD45 expression in pediatric ALL patients treated according to he ALL-IC-BFM 2009 protocol.
Methods
The brightness of CD45 expression was compared in ten patients of high risk group and ten patient of standart and intermediate groups. In the ALL-IC BFM 2009 trial, the high-risk group was defined by poor prednisone response on treatment day 8, FCM blast ratio >%10 at day 15 and BCR-ABL OR MLL positivity. The brightness of CD45 expression was evaluated as mean flouresence index (MFI).
Results
CD45 MFI was 710,2 for blast, 13784,6 for normal lymphocytes, 0,056 for blast/lymphocytes in hr group, CD45 MFI was 590 for blast, 7904,4 for normal lymphocytes, 0,082 fr blast/lymphocytes in hr group. The differenceb between groups was not significantly different.
Summary
In conclusion, the CD45 brightnes of childhood BCP-ALL did nod have any additional contribution to high risk stratification. This result is different from previous reports. New reports is required to solve this debate.
Session topic: Publication Only
Type: Publication Only
Background
Two report shows CD45 brightnes of childhood ALL blast is associated with poor prognosis. Borowitz et al showed that bright CD45 expression was associated with a poor prognosis in BCP-ALL patients treated according to theprotocols of the Pediatric Oncology Groupstudy group and Cario et al also in ALL-BFM-2000 protocol.
Aims
In the present study, we assessed the relation of brightness of CD45 expression in pediatric ALL patients treated according to he ALL-IC-BFM 2009 protocol.
Methods
The brightness of CD45 expression was compared in ten patients of high risk group and ten patient of standart and intermediate groups. In the ALL-IC BFM 2009 trial, the high-risk group was defined by poor prednisone response on treatment day 8, FCM blast ratio >%10 at day 15 and BCR-ABL OR MLL positivity. The brightness of CD45 expression was evaluated as mean flouresence index (MFI).
Results
CD45 MFI was 710,2 for blast, 13784,6 for normal lymphocytes, 0,056 for blast/lymphocytes in hr group, CD45 MFI was 590 for blast, 7904,4 for normal lymphocytes, 0,082 fr blast/lymphocytes in hr group. The differenceb between groups was not significantly different.
Summary
In conclusion, the CD45 brightnes of childhood BCP-ALL did nod have any additional contribution to high risk stratification. This result is different from previous reports. New reports is required to solve this debate.
Session topic: Publication Only
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