INVESTIGATION OF SEROUS EFFUSIONS WITH FLOW CYTOMETRY
(Abstract release date: 05/21/15)
EHA Library. Iltar U. 06/12/15; 102927; PB1677
Utku Iltar
Contributions
Contributions
Abstract
Abstract: PB1677
Type: Publication Only
Background
In current clinical practice serous effusions are evaluated by cytopathologic, chemical and microbiologic examinations. As well as infections and inflammatory diseases, serous body fluids can also be observed in hematopoietic and solid neoplastic diseases. Flow cytometry(FC) is well established as a critical and quick diagnostik tool used routinely in the diagnosis of hematopoietic neoplasms by fine-needle aspiration and an alternative diagnostic method for the evaluation of serous body fluids.
Aims
The aim of this study was to investigate immunophenotypic analysis of serous body fluids by FC.
Methods
During the time period of 2010-2014, a total of 79 consecutive serous effusion specimens (45 pleural, 33 peritoneal, 1 pericardial ) of 76 cases (40 female, 36 male, 24-84 years of age) were investigated with the six-color FC (FACSCanto II, BD Biosystems, San Jose, USA) in the Division of Hematology Laboratory at Akdeniz University Hospital. We retrospectively analyzed these datas.
Results
FC was positive for definitive immunophenotypic evidence of a hematopoietic malignancy in 3 of 45 pleural fluid specimens (Diffuse Large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case), T-cell Acute Lymphoblastic Leukemia / Lymphoma(1 case)) and 3 of 33 peritoneal fluid specimens (T-cell prolymphocytic leukemia(1 case), diffuse large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case)(Figure 1)). Subsequent tissue studies confirmed the diagnosis of a hematopoietic neoplasm in these seperate six cases.
Summary
Cytopathologic evaluation may lay long especially in the diagnosis of aggressive lymphomas which requires urgent evaluation and treatment. Immunophenotypic analysis of serous body fluids by FC can provide better access to early diagnosis and also accurate clinical staging.
Keyword(s): Flow cytometry, Lymphoma
Session topic: Publication Only
Type: Publication Only
Background
In current clinical practice serous effusions are evaluated by cytopathologic, chemical and microbiologic examinations. As well as infections and inflammatory diseases, serous body fluids can also be observed in hematopoietic and solid neoplastic diseases. Flow cytometry(FC) is well established as a critical and quick diagnostik tool used routinely in the diagnosis of hematopoietic neoplasms by fine-needle aspiration and an alternative diagnostic method for the evaluation of serous body fluids.
Aims
The aim of this study was to investigate immunophenotypic analysis of serous body fluids by FC.
Methods
During the time period of 2010-2014, a total of 79 consecutive serous effusion specimens (45 pleural, 33 peritoneal, 1 pericardial ) of 76 cases (40 female, 36 male, 24-84 years of age) were investigated with the six-color FC (FACSCanto II, BD Biosystems, San Jose, USA) in the Division of Hematology Laboratory at Akdeniz University Hospital. We retrospectively analyzed these datas.
Results
FC was positive for definitive immunophenotypic evidence of a hematopoietic malignancy in 3 of 45 pleural fluid specimens (Diffuse Large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case), T-cell Acute Lymphoblastic Leukemia / Lymphoma(1 case)) and 3 of 33 peritoneal fluid specimens (T-cell prolymphocytic leukemia(1 case), diffuse large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case)(Figure 1)). Subsequent tissue studies confirmed the diagnosis of a hematopoietic neoplasm in these seperate six cases.
Summary
Cytopathologic evaluation may lay long especially in the diagnosis of aggressive lymphomas which requires urgent evaluation and treatment. Immunophenotypic analysis of serous body fluids by FC can provide better access to early diagnosis and also accurate clinical staging.
Keyword(s): Flow cytometry, Lymphoma
Session topic: Publication Only
Abstract: PB1677
Type: Publication Only
Background
In current clinical practice serous effusions are evaluated by cytopathologic, chemical and microbiologic examinations. As well as infections and inflammatory diseases, serous body fluids can also be observed in hematopoietic and solid neoplastic diseases. Flow cytometry(FC) is well established as a critical and quick diagnostik tool used routinely in the diagnosis of hematopoietic neoplasms by fine-needle aspiration and an alternative diagnostic method for the evaluation of serous body fluids.
Aims
The aim of this study was to investigate immunophenotypic analysis of serous body fluids by FC.
Methods
During the time period of 2010-2014, a total of 79 consecutive serous effusion specimens (45 pleural, 33 peritoneal, 1 pericardial ) of 76 cases (40 female, 36 male, 24-84 years of age) were investigated with the six-color FC (FACSCanto II, BD Biosystems, San Jose, USA) in the Division of Hematology Laboratory at Akdeniz University Hospital. We retrospectively analyzed these datas.
Results
FC was positive for definitive immunophenotypic evidence of a hematopoietic malignancy in 3 of 45 pleural fluid specimens (Diffuse Large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case), T-cell Acute Lymphoblastic Leukemia / Lymphoma(1 case)) and 3 of 33 peritoneal fluid specimens (T-cell prolymphocytic leukemia(1 case), diffuse large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case)(Figure 1)). Subsequent tissue studies confirmed the diagnosis of a hematopoietic neoplasm in these seperate six cases.
Summary
Cytopathologic evaluation may lay long especially in the diagnosis of aggressive lymphomas which requires urgent evaluation and treatment. Immunophenotypic analysis of serous body fluids by FC can provide better access to early diagnosis and also accurate clinical staging.
Keyword(s): Flow cytometry, Lymphoma
Session topic: Publication Only
Type: Publication Only
Background
In current clinical practice serous effusions are evaluated by cytopathologic, chemical and microbiologic examinations. As well as infections and inflammatory diseases, serous body fluids can also be observed in hematopoietic and solid neoplastic diseases. Flow cytometry(FC) is well established as a critical and quick diagnostik tool used routinely in the diagnosis of hematopoietic neoplasms by fine-needle aspiration and an alternative diagnostic method for the evaluation of serous body fluids.
Aims
The aim of this study was to investigate immunophenotypic analysis of serous body fluids by FC.
Methods
During the time period of 2010-2014, a total of 79 consecutive serous effusion specimens (45 pleural, 33 peritoneal, 1 pericardial ) of 76 cases (40 female, 36 male, 24-84 years of age) were investigated with the six-color FC (FACSCanto II, BD Biosystems, San Jose, USA) in the Division of Hematology Laboratory at Akdeniz University Hospital. We retrospectively analyzed these datas.
Results
FC was positive for definitive immunophenotypic evidence of a hematopoietic malignancy in 3 of 45 pleural fluid specimens (Diffuse Large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case), T-cell Acute Lymphoblastic Leukemia / Lymphoma(1 case)) and 3 of 33 peritoneal fluid specimens (T-cell prolymphocytic leukemia(1 case), diffuse large B-cell lymphoma(1 case), Burkitt's lymphoma(1 case)(Figure 1)). Subsequent tissue studies confirmed the diagnosis of a hematopoietic neoplasm in these seperate six cases.
Summary
Cytopathologic evaluation may lay long especially in the diagnosis of aggressive lymphomas which requires urgent evaluation and treatment. Immunophenotypic analysis of serous body fluids by FC can provide better access to early diagnosis and also accurate clinical staging.
Keyword(s): Flow cytometry, Lymphoma
Session topic: Publication Only
{{ help_message }}
{{filter}}