Laboratory of Haematology
Contributions
Type: Publication Only
Background
Background.Multiple myeloma (MM) is a malignant proliferation of monoclonal plasma cells, resulting in a variety of clinical manifestations including osteolytic bone lesions, anemia, hypercalcemia and renal failure. Angiogenesis, the formation of new blood vessels from existing blood vessels,is an essential component in the growth and progression of MM.
Fms-like tyrosine kinase 3 (Flt3) ligand (Flt3-L) is a potent hematopoietic cytokine that is probably involved in early B cell development and it is expressed by endothelial cells.
Aims
Aims.The aim of the present study was to evaluate serum levels of circulating FLT3-L in newly diagnosed MM patients and to estimate if there is any relationship between FLT3-L and known markers of angiogenesis such as ,TNFa,HGF, and the expression of CD105 on endothelial cells.
Methods
Methods.We studied 56 newly diagnosed MM patients (26 female and 30 male with mean age 64.5±12.3 years), according to ISS .15 had stage- I disease,19 stage -II and 22 stage- III. We also studied 20 healthy persons as control group.TNFa ,HGF and FLT3-L serum levels,were determinate by a solid-phase sandwich ELISA,using commercially available kits.CD105 expression was determinate by immunohistochemical methods.
Results
Results.FLT3-L,TNFa,HGF and expression of CD105 values,were significantly higher in MM patients compare to controls(p<0,001 in all cases),TNFa,FLT3-L,HGF and CD105 values,were also significantly higher with advancing disease stage(p<0,001 in all cases).
We also found significant correlations between FLT3-L serum levels with expression of CD105 values (r=0,459,p<0,0001),TNFa (r=0,458,p<0,0001) and HGF (r=0,537,p<0,0001).
Summary
Conclusion.There is a growing evidence that FLT3-L has a significant role in the progression of MM.Serum levels of the angiogenic cytokines TNFa,HGF and the expression of CD105 wich reflect bone marrow neovascularisation,are increased in MM patients and correlated strongly with FLT3-L values this findings indicates that FLT3-L serum levels could be used as a potential tumor marker for disease severty and angiogenesis.
Keyword(s): Angiogenesis, Cytokine, Multiple myeloma
Type: Publication Only
Background
Background.Multiple myeloma (MM) is a malignant proliferation of monoclonal plasma cells, resulting in a variety of clinical manifestations including osteolytic bone lesions, anemia, hypercalcemia and renal failure. Angiogenesis, the formation of new blood vessels from existing blood vessels,is an essential component in the growth and progression of MM.
Fms-like tyrosine kinase 3 (Flt3) ligand (Flt3-L) is a potent hematopoietic cytokine that is probably involved in early B cell development and it is expressed by endothelial cells.
Aims
Aims.The aim of the present study was to evaluate serum levels of circulating FLT3-L in newly diagnosed MM patients and to estimate if there is any relationship between FLT3-L and known markers of angiogenesis such as ,TNFa,HGF, and the expression of CD105 on endothelial cells.
Methods
Methods.We studied 56 newly diagnosed MM patients (26 female and 30 male with mean age 64.5±12.3 years), according to ISS .15 had stage- I disease,19 stage -II and 22 stage- III. We also studied 20 healthy persons as control group.TNFa ,HGF and FLT3-L serum levels,were determinate by a solid-phase sandwich ELISA,using commercially available kits.CD105 expression was determinate by immunohistochemical methods.
Results
Results.FLT3-L,TNFa,HGF and expression of CD105 values,were significantly higher in MM patients compare to controls(p<0,001 in all cases),TNFa,FLT3-L,HGF and CD105 values,were also significantly higher with advancing disease stage(p<0,001 in all cases).
We also found significant correlations between FLT3-L serum levels with expression of CD105 values (r=0,459,p<0,0001),TNFa (r=0,458,p<0,0001) and HGF (r=0,537,p<0,0001).
Summary
Conclusion.There is a growing evidence that FLT3-L has a significant role in the progression of MM.Serum levels of the angiogenic cytokines TNFa,HGF and the expression of CD105 wich reflect bone marrow neovascularisation,are increased in MM patients and correlated strongly with FLT3-L values this findings indicates that FLT3-L serum levels could be used as a potential tumor marker for disease severty and angiogenesis.
Keyword(s): Angiogenesis, Cytokine, Multiple myeloma