T REGULATORY CELLS IN CHILDHOOD MALIGNANCIES
(Abstract release date: 05/21/15)
EHA Library. Stratigaki M. 06/12/15; 102863; PB1943
Dr. Maria Stratigaki
Contributions
Contributions
Abstract
Abstract: PB1943
Type: Publication Only
Background
T regulatory cells (T regs) participate in the maintenance of immunological self-tolerance and according recent studies play an essential role for the control of antitumor immune responses. The utility of T regs as prognostic factors and targeted treatment strategies in different cancers is under immense investigation.
Aims
The study of T regulatory cells’ subpopulations CD4+CD25high and CD4+CD25highFOXP3+ in the peripheral blood of children with malignancies (lymphomas and soft tissue sarcomas) at diagnosis, during therapy and after treatment completion as well as of children with autoimmune cytopenias.
Methods
Mononuclear cells were isolated from peripheral blood (PBMCs) of children with malignancies at diagnosis (Md, n=9), during therapy (T, n=8) and at the end of treatment (ET, n=26) as well as of children with autoimmune cytopenia (CP, n=6) by density gradient centrifugation. A multi-color flow cytometry panel was used for the validation of T reg subpopulations using CD4-PC5, CD25-PE and the intracellular FOXP3-FITC monoclonal antibodies. The panel for the estimation of CD4/CD8 ratio included CD45-PC7, CD3-FITC, CD4-PE and CD8-PC5.
Results
The findings of our study revealed that the levels of CD4+CD25high and CD4+CD25highFOXP3+ subpopulations of T regulatory cells were low at diagnosis of malignancies. According to our analysis an increase of CD4+CD25high and CD4+CD25highFOXP3+ levels (0.5±0.094 vs 1.08±0.187, p=0.015 and 0.390± 0.087 vs 0.84±0.173, p=0.049 respectively) were observed during treatment administration and a new reduction of the above subpopulations after treatment completion (1.08±0.187 vs 0.62±0.062, p=0.034 and 0.84±0.173 vs 0.46±0.063, p=0.031 respectively). T reg CD4+CD25highFOXP3+ cells were also elevated during treatment compared to cytopenias (0.84±0.173 vs 0.36±0.065, p=0.041). No statistically significant differences were estimated from the comparison between the group of cytopenias and the diagnosis of malignancies as well as the end of treatment. In addition CD4/CD8 ratio was significantly higher at diagnosis of malignancies compared to treatment period and the end of treatment group (2.13±0.299 vs 1.29±0.207, p=0.043 and 2.13±0.299 vs 1.4±0.085, p=0.019 respectively).
Summary
In childhood malignancies studied the levels of T regulatory cells were estimated low at diagnosis and after treatment completion whereas were elevated under treatment as a result related to immunosuppression.
Keyword(s): Childhood, Lymphoma, Malignancy, T regulatory cells
Session topic: Publication Only
Type: Publication Only
Background
T regulatory cells (T regs) participate in the maintenance of immunological self-tolerance and according recent studies play an essential role for the control of antitumor immune responses. The utility of T regs as prognostic factors and targeted treatment strategies in different cancers is under immense investigation.
Aims
The study of T regulatory cells’ subpopulations CD4+CD25high and CD4+CD25highFOXP3+ in the peripheral blood of children with malignancies (lymphomas and soft tissue sarcomas) at diagnosis, during therapy and after treatment completion as well as of children with autoimmune cytopenias.
Methods
Mononuclear cells were isolated from peripheral blood (PBMCs) of children with malignancies at diagnosis (Md, n=9), during therapy (T, n=8) and at the end of treatment (ET, n=26) as well as of children with autoimmune cytopenia (CP, n=6) by density gradient centrifugation. A multi-color flow cytometry panel was used for the validation of T reg subpopulations using CD4-PC5, CD25-PE and the intracellular FOXP3-FITC monoclonal antibodies. The panel for the estimation of CD4/CD8 ratio included CD45-PC7, CD3-FITC, CD4-PE and CD8-PC5.
Results
The findings of our study revealed that the levels of CD4+CD25high and CD4+CD25highFOXP3+ subpopulations of T regulatory cells were low at diagnosis of malignancies. According to our analysis an increase of CD4+CD25high and CD4+CD25highFOXP3+ levels (0.5±0.094 vs 1.08±0.187, p=0.015 and 0.390± 0.087 vs 0.84±0.173, p=0.049 respectively) were observed during treatment administration and a new reduction of the above subpopulations after treatment completion (1.08±0.187 vs 0.62±0.062, p=0.034 and 0.84±0.173 vs 0.46±0.063, p=0.031 respectively). T reg CD4+CD25highFOXP3+ cells were also elevated during treatment compared to cytopenias (0.84±0.173 vs 0.36±0.065, p=0.041). No statistically significant differences were estimated from the comparison between the group of cytopenias and the diagnosis of malignancies as well as the end of treatment. In addition CD4/CD8 ratio was significantly higher at diagnosis of malignancies compared to treatment period and the end of treatment group (2.13±0.299 vs 1.29±0.207, p=0.043 and 2.13±0.299 vs 1.4±0.085, p=0.019 respectively).
Summary
In childhood malignancies studied the levels of T regulatory cells were estimated low at diagnosis and after treatment completion whereas were elevated under treatment as a result related to immunosuppression.
Keyword(s): Childhood, Lymphoma, Malignancy, T regulatory cells
Session topic: Publication Only
Abstract: PB1943
Type: Publication Only
Background
T regulatory cells (T regs) participate in the maintenance of immunological self-tolerance and according recent studies play an essential role for the control of antitumor immune responses. The utility of T regs as prognostic factors and targeted treatment strategies in different cancers is under immense investigation.
Aims
The study of T regulatory cells’ subpopulations CD4+CD25high and CD4+CD25highFOXP3+ in the peripheral blood of children with malignancies (lymphomas and soft tissue sarcomas) at diagnosis, during therapy and after treatment completion as well as of children with autoimmune cytopenias.
Methods
Mononuclear cells were isolated from peripheral blood (PBMCs) of children with malignancies at diagnosis (Md, n=9), during therapy (T, n=8) and at the end of treatment (ET, n=26) as well as of children with autoimmune cytopenia (CP, n=6) by density gradient centrifugation. A multi-color flow cytometry panel was used for the validation of T reg subpopulations using CD4-PC5, CD25-PE and the intracellular FOXP3-FITC monoclonal antibodies. The panel for the estimation of CD4/CD8 ratio included CD45-PC7, CD3-FITC, CD4-PE and CD8-PC5.
Results
The findings of our study revealed that the levels of CD4+CD25high and CD4+CD25highFOXP3+ subpopulations of T regulatory cells were low at diagnosis of malignancies. According to our analysis an increase of CD4+CD25high and CD4+CD25highFOXP3+ levels (0.5±0.094 vs 1.08±0.187, p=0.015 and 0.390± 0.087 vs 0.84±0.173, p=0.049 respectively) were observed during treatment administration and a new reduction of the above subpopulations after treatment completion (1.08±0.187 vs 0.62±0.062, p=0.034 and 0.84±0.173 vs 0.46±0.063, p=0.031 respectively). T reg CD4+CD25highFOXP3+ cells were also elevated during treatment compared to cytopenias (0.84±0.173 vs 0.36±0.065, p=0.041). No statistically significant differences were estimated from the comparison between the group of cytopenias and the diagnosis of malignancies as well as the end of treatment. In addition CD4/CD8 ratio was significantly higher at diagnosis of malignancies compared to treatment period and the end of treatment group (2.13±0.299 vs 1.29±0.207, p=0.043 and 2.13±0.299 vs 1.4±0.085, p=0.019 respectively).
Summary
In childhood malignancies studied the levels of T regulatory cells were estimated low at diagnosis and after treatment completion whereas were elevated under treatment as a result related to immunosuppression.
Keyword(s): Childhood, Lymphoma, Malignancy, T regulatory cells
Session topic: Publication Only
Type: Publication Only
Background
T regulatory cells (T regs) participate in the maintenance of immunological self-tolerance and according recent studies play an essential role for the control of antitumor immune responses. The utility of T regs as prognostic factors and targeted treatment strategies in different cancers is under immense investigation.
Aims
The study of T regulatory cells’ subpopulations CD4+CD25high and CD4+CD25highFOXP3+ in the peripheral blood of children with malignancies (lymphomas and soft tissue sarcomas) at diagnosis, during therapy and after treatment completion as well as of children with autoimmune cytopenias.
Methods
Mononuclear cells were isolated from peripheral blood (PBMCs) of children with malignancies at diagnosis (Md, n=9), during therapy (T, n=8) and at the end of treatment (ET, n=26) as well as of children with autoimmune cytopenia (CP, n=6) by density gradient centrifugation. A multi-color flow cytometry panel was used for the validation of T reg subpopulations using CD4-PC5, CD25-PE and the intracellular FOXP3-FITC monoclonal antibodies. The panel for the estimation of CD4/CD8 ratio included CD45-PC7, CD3-FITC, CD4-PE and CD8-PC5.
Results
The findings of our study revealed that the levels of CD4+CD25high and CD4+CD25highFOXP3+ subpopulations of T regulatory cells were low at diagnosis of malignancies. According to our analysis an increase of CD4+CD25high and CD4+CD25highFOXP3+ levels (0.5±0.094 vs 1.08±0.187, p=0.015 and 0.390± 0.087 vs 0.84±0.173, p=0.049 respectively) were observed during treatment administration and a new reduction of the above subpopulations after treatment completion (1.08±0.187 vs 0.62±0.062, p=0.034 and 0.84±0.173 vs 0.46±0.063, p=0.031 respectively). T reg CD4+CD25highFOXP3+ cells were also elevated during treatment compared to cytopenias (0.84±0.173 vs 0.36±0.065, p=0.041). No statistically significant differences were estimated from the comparison between the group of cytopenias and the diagnosis of malignancies as well as the end of treatment. In addition CD4/CD8 ratio was significantly higher at diagnosis of malignancies compared to treatment period and the end of treatment group (2.13±0.299 vs 1.29±0.207, p=0.043 and 2.13±0.299 vs 1.4±0.085, p=0.019 respectively).
Summary
In childhood malignancies studied the levels of T regulatory cells were estimated low at diagnosis and after treatment completion whereas were elevated under treatment as a result related to immunosuppression.
Keyword(s): Childhood, Lymphoma, Malignancy, T regulatory cells
Session topic: Publication Only
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