Heamatology
Contributions
Type: Publication Only
Background
It is common knowledge that the FBC has to be analyzed as soon as possible to get an accurate result. However due to the heavy workload and the increase demand for FBC, a delay before analysis is seen many times. Therefore it is vital to know the storage effects that are likely to affect the FBC.
Aims
FBC is a laboratory test which is routinely done as one of the first steps in the diagnostic workup. There is often a post collection interval before analysis of results where external factors such as storage temperature, and the duration of sample storage can affect the results. This study analyses the storage effects of components of FBC at different temperatures over time.
Methods
A descriptive cross sectional study was conducted in the laboratory of CSTH. K2EDTA blood samples were randomly taken from 102 apparently healthy individuals aged between 20 - 70 years. Baseline measurements were analyzed within 30 min of collection through a fully automated haematology analyzer Sysmex SX 500i. Samples were divided into 3 portions and each of them was kept at 4±2?C, 23±2?C and 31±2?C up to 48hrs. All were repeatedly analyzed after 6hrs, 24hrs, and 48hrs. Statistical analysis was conducted using SPSS 17.O
Results
Among parameters of the FBC, red cell count and haemoglobin were stable at 40 C, 23±2?C and 31±2?C throughout the study (48 hrs).White blood cell count (WBC) was increased at 31±2?C and 23±2?C. Machine refused to read the differential count of some samples after 24hr of storage. Neutrophil and Basophil counts were significantly increased with time throughout the study in all temperatures. Storage caused a significant decline in platelet count and an increment in MPV at all temperatures after 6hr. (P <0.05). At 40C MCV and RDW were decreased whereas it was significantly increased at RT and 23±2?C.
Summary
In order to prevent variability in FBC parameters, it is preferred to analyze the blood sample as soon as possible. For an accurate platelet count, samples should be analyzed within 6hr. Haemoglobin remains stable at any temperature even at 48 hr. Neutrophil count keeps increasing with storage time at different temperatures. Differential counts were rejected by the machine in most cases after 1day of storage.
Type: Publication Only
Background
It is common knowledge that the FBC has to be analyzed as soon as possible to get an accurate result. However due to the heavy workload and the increase demand for FBC, a delay before analysis is seen many times. Therefore it is vital to know the storage effects that are likely to affect the FBC.
Aims
FBC is a laboratory test which is routinely done as one of the first steps in the diagnostic workup. There is often a post collection interval before analysis of results where external factors such as storage temperature, and the duration of sample storage can affect the results. This study analyses the storage effects of components of FBC at different temperatures over time.
Methods
A descriptive cross sectional study was conducted in the laboratory of CSTH. K2EDTA blood samples were randomly taken from 102 apparently healthy individuals aged between 20 - 70 years. Baseline measurements were analyzed within 30 min of collection through a fully automated haematology analyzer Sysmex SX 500i. Samples were divided into 3 portions and each of them was kept at 4±2?C, 23±2?C and 31±2?C up to 48hrs. All were repeatedly analyzed after 6hrs, 24hrs, and 48hrs. Statistical analysis was conducted using SPSS 17.O
Results
Among parameters of the FBC, red cell count and haemoglobin were stable at 40 C, 23±2?C and 31±2?C throughout the study (48 hrs).White blood cell count (WBC) was increased at 31±2?C and 23±2?C. Machine refused to read the differential count of some samples after 24hr of storage. Neutrophil and Basophil counts were significantly increased with time throughout the study in all temperatures. Storage caused a significant decline in platelet count and an increment in MPV at all temperatures after 6hr. (P <0.05). At 40C MCV and RDW were decreased whereas it was significantly increased at RT and 23±2?C.
Summary
In order to prevent variability in FBC parameters, it is preferred to analyze the blood sample as soon as possible. For an accurate platelet count, samples should be analyzed within 6hr. Haemoglobin remains stable at any temperature even at 48 hr. Neutrophil count keeps increasing with storage time at different temperatures. Differential counts were rejected by the machine in most cases after 1day of storage.