Pathophysiology
Contributions
Type: Publication Only
Background
Chronic Idiopathic Thrombocytopenic Purpura (ITP) is an autoimmune disease characterised by a low platelet count determined by an increased platelet destruction or a decreased platelet production. Oxidative stress, defined as an imbalance between the reactive oxygen species and the antioxidant defence system, plays an important role in the pathophysiology of autoimmune diseases determined by DNA, protein and lipid oxidation.
Aims
To evaluate if oxidative stress is involved in the pathogenesis of chronic ITP.
Methods
We studied 28 patients with chronic ITP hospitalized in the Clinic of Hematology of Craiova (Romania) between 2012 and 2014 (group A). The A group was compared to 30 healthy people who represented the control group (group B). Both free oxygen radicals and total antioxidant capacity were evaluated by FORT (Free Oxygen Radicals testing) and FORD (Free Oxygen Radical Defence) tests from a single drop of capillary blood, at the time of diagnosis, before the administration of any drug. The normal value of FORT was less than 2.3 mmol/L H2O2 and the normal value of FORD was in between 1.07 - 1.53 mmol/L. The statistical analysis was performed and a p value ≤ 0.001 was considered significant.
Results
A statistically significant difference was determined for both FORT and FORD levels between the two groups. The FORT and FORD levels were higher in group A compared with group B.
Summary
We believe that oxidative stress is involved in the pathophysiology of the chronic ITP. When free oxygen radicals become excessive and surpass the total antioxidant defence capacity, they are destructive and attack the fundamental cellular components such as proteins that may be highly immunogenic and induce autoantibody production which is involved in platelet destruction.
Keyword(s): Idiopathic thombocytopenic purpura (ITP), Platelet count, Reactive oxygen species
Session topic: Publication Only
Type: Publication Only
Background
Chronic Idiopathic Thrombocytopenic Purpura (ITP) is an autoimmune disease characterised by a low platelet count determined by an increased platelet destruction or a decreased platelet production. Oxidative stress, defined as an imbalance between the reactive oxygen species and the antioxidant defence system, plays an important role in the pathophysiology of autoimmune diseases determined by DNA, protein and lipid oxidation.
Aims
To evaluate if oxidative stress is involved in the pathogenesis of chronic ITP.
Methods
We studied 28 patients with chronic ITP hospitalized in the Clinic of Hematology of Craiova (Romania) between 2012 and 2014 (group A). The A group was compared to 30 healthy people who represented the control group (group B). Both free oxygen radicals and total antioxidant capacity were evaluated by FORT (Free Oxygen Radicals testing) and FORD (Free Oxygen Radical Defence) tests from a single drop of capillary blood, at the time of diagnosis, before the administration of any drug. The normal value of FORT was less than 2.3 mmol/L H2O2 and the normal value of FORD was in between 1.07 - 1.53 mmol/L. The statistical analysis was performed and a p value ≤ 0.001 was considered significant.
Results
A statistically significant difference was determined for both FORT and FORD levels between the two groups. The FORT and FORD levels were higher in group A compared with group B.
Summary
We believe that oxidative stress is involved in the pathophysiology of the chronic ITP. When free oxygen radicals become excessive and surpass the total antioxidant defence capacity, they are destructive and attack the fundamental cellular components such as proteins that may be highly immunogenic and induce autoantibody production which is involved in platelet destruction.
Keyword(s): Idiopathic thombocytopenic purpura (ITP), Platelet count, Reactive oxygen species
Session topic: Publication Only