Contributions
Type: Publication Only
Background
Bone marrow angiogenesis is involved in the pathogenesis and progression of certain haematological malignancies, like multiple myeloma (MM). Increased mast cell density (MCD) in bone marrow (BM) produces various mediators promoting MM progression with multiple manners.
Aims
The purpose of this study was to evaluate the involvement of BM MCD, and angiogenic cytokines such as angiopoietin-2(angiop-2), angiogenin (ang) and metalloproteinase-9(MMP-9) in MM disease progression.
Methods
We studied 70 newly diagnosed MM patients, 38 males, 32 females, mean age 57±14,5 years.According to ISS 20 were in stage I, 28 in stage II and 22 in stage III. 20 age and sex matched healthy volunteers were used as control group. The immunohistochemical expression stage of mast cell thryptase was measured in BM biopsy samples in order to estimate MCD, while angiop-2, MMP-9 and ang values were measured in serum by a solid phase sandwich ELISA.
Results
There are significant differences in levels of MCD, Angiop-2 and ANG (p<0,001 for all cases) but no significant differences in MMP-9(p=0,538) between patients and controls. All of the measured parameters were also in parallel with ISS stage(p<0,001 for all cases). Moreover BM MCD correlated positively with angiop-2, MMP-9, and ang (p<0,0001 for all cases).
Summary
Mast cells are increased in MM BM and they participate in many aspects of the disease. They release various mediators, increasing directly and indirectly myeloma growth.It has been also established that disease progression in MM is accompanied by increased BM angiogenesis. Overall mast cells and angiogenic growth factors seem to be important factors in MM biology and disease progression.
Keyword(s): Angiogenesis, Bone disease, Cytokine, Multiple myeloma
Session topic: Publication Only
Type: Publication Only
Background
Bone marrow angiogenesis is involved in the pathogenesis and progression of certain haematological malignancies, like multiple myeloma (MM). Increased mast cell density (MCD) in bone marrow (BM) produces various mediators promoting MM progression with multiple manners.
Aims
The purpose of this study was to evaluate the involvement of BM MCD, and angiogenic cytokines such as angiopoietin-2(angiop-2), angiogenin (ang) and metalloproteinase-9(MMP-9) in MM disease progression.
Methods
We studied 70 newly diagnosed MM patients, 38 males, 32 females, mean age 57±14,5 years.According to ISS 20 were in stage I, 28 in stage II and 22 in stage III. 20 age and sex matched healthy volunteers were used as control group. The immunohistochemical expression stage of mast cell thryptase was measured in BM biopsy samples in order to estimate MCD, while angiop-2, MMP-9 and ang values were measured in serum by a solid phase sandwich ELISA.
Results
There are significant differences in levels of MCD, Angiop-2 and ANG (p<0,001 for all cases) but no significant differences in MMP-9(p=0,538) between patients and controls. All of the measured parameters were also in parallel with ISS stage(p<0,001 for all cases). Moreover BM MCD correlated positively with angiop-2, MMP-9, and ang (p<0,0001 for all cases).
Summary
Mast cells are increased in MM BM and they participate in many aspects of the disease. They release various mediators, increasing directly and indirectly myeloma growth.It has been also established that disease progression in MM is accompanied by increased BM angiogenesis. Overall mast cells and angiogenic growth factors seem to be important factors in MM biology and disease progression.
Keyword(s): Angiogenesis, Bone disease, Cytokine, Multiple myeloma
Session topic: Publication Only