THE EFFECT OF PROLIFERATION INHIBITION AND APOPTOSIS OF BRD4 INHIBITORS JQ1 ON PH POSITIVE ACUTE LYMPHOCYTIC LEUKEMIA CELL AND ITS MECHANISM
(Abstract release date: 05/21/15)
EHA Library. Liangming M. 06/12/15; 102587; PB1596
Disclosure(s): SHAN XI DA YI HOSPITALHEMATOLOGY
Ma Liangming
Contributions
Contributions
Abstract
Abstract: PB1596
Type: Publication Only
Background
It has been widely demonstrated that BRD4 knockdown or inhibition by JQ1 is associated with c-MYC downregulation and antileukemic activity. leading to suppression of the transcriptional program linked to proliferation and survival.
Aims
We evaluated the effect of proliferation inhibition and apoptosis of brd4 inhibitor JQ1 on Ph positive acute lymphocytic leukemia(Ph+ ALL) cell and its mechanism.
Methods
different concentrations of JQ1 are used on SUP - B15 cell?and proliferation inhibition level were detected by MTT assay?the cell apoptosis rate were determined by flow cytometry(FCM)?the expressions of BCR – ABLmRNA?brd4mRNA?mycmRNA?P53mRNA were detected by real-time fluorescent quantitative PCR(RT-PCR).
Results
different concentrations of JQ1 can all inhibit SUP - B15 cell proliferation, inducing cell apoptosis?apoptosis rate was significantly increased compared to control group, and depend of time and dose. half inhibitory concentration of 72h is about 1.0 umol/L.At the same time?JQ1 can down regulation BCR-ABLmRNA,brd4 mRNA, myc mRNA transcription levels, and up regulation the transcription level of p53mRNA .
Summary
Keyword(s): B cell acute lymphoblastic leukemia, C-myc, Leukemia cell line, P53
Type: Publication Only
Background
It has been widely demonstrated that BRD4 knockdown or inhibition by JQ1 is associated with c-MYC downregulation and antileukemic activity. leading to suppression of the transcriptional program linked to proliferation and survival.
Aims
We evaluated the effect of proliferation inhibition and apoptosis of brd4 inhibitor JQ1 on Ph positive acute lymphocytic leukemia(Ph+ ALL) cell and its mechanism.
Methods
different concentrations of JQ1 are used on SUP - B15 cell?and proliferation inhibition level were detected by MTT assay?the cell apoptosis rate were determined by flow cytometry(FCM)?the expressions of BCR – ABLmRNA?brd4mRNA?mycmRNA?P53mRNA were detected by real-time fluorescent quantitative PCR(RT-PCR).
Results
different concentrations of JQ1 can all inhibit SUP - B15 cell proliferation, inducing cell apoptosis?apoptosis rate was significantly increased compared to control group, and depend of time and dose. half inhibitory concentration of 72h is about 1.0 umol/L.At the same time?JQ1 can down regulation BCR-ABLmRNA,brd4 mRNA, myc mRNA transcription levels, and up regulation the transcription level of p53mRNA .
Summary
JQ1 as brd4 inhibitor can down regulation the expression of brd4, thus affecting its downstream gene myc and p53, at the same time it can changeover the express of BCR-ABL,further achieve inhibition of cell proliferation, inducing cell apoptosis.
Keyword(s): B cell acute lymphoblastic leukemia, C-myc, Leukemia cell line, P53
Abstract: PB1596
Type: Publication Only
Background
It has been widely demonstrated that BRD4 knockdown or inhibition by JQ1 is associated with c-MYC downregulation and antileukemic activity. leading to suppression of the transcriptional program linked to proliferation and survival.
Aims
We evaluated the effect of proliferation inhibition and apoptosis of brd4 inhibitor JQ1 on Ph positive acute lymphocytic leukemia(Ph+ ALL) cell and its mechanism.
Methods
different concentrations of JQ1 are used on SUP - B15 cell?and proliferation inhibition level were detected by MTT assay?the cell apoptosis rate were determined by flow cytometry(FCM)?the expressions of BCR – ABLmRNA?brd4mRNA?mycmRNA?P53mRNA were detected by real-time fluorescent quantitative PCR(RT-PCR).
Results
different concentrations of JQ1 can all inhibit SUP - B15 cell proliferation, inducing cell apoptosis?apoptosis rate was significantly increased compared to control group, and depend of time and dose. half inhibitory concentration of 72h is about 1.0 umol/L.At the same time?JQ1 can down regulation BCR-ABLmRNA,brd4 mRNA, myc mRNA transcription levels, and up regulation the transcription level of p53mRNA .
Summary
Keyword(s): B cell acute lymphoblastic leukemia, C-myc, Leukemia cell line, P53
Type: Publication Only
Background
It has been widely demonstrated that BRD4 knockdown or inhibition by JQ1 is associated with c-MYC downregulation and antileukemic activity. leading to suppression of the transcriptional program linked to proliferation and survival.
Aims
We evaluated the effect of proliferation inhibition and apoptosis of brd4 inhibitor JQ1 on Ph positive acute lymphocytic leukemia(Ph+ ALL) cell and its mechanism.
Methods
different concentrations of JQ1 are used on SUP - B15 cell?and proliferation inhibition level were detected by MTT assay?the cell apoptosis rate were determined by flow cytometry(FCM)?the expressions of BCR – ABLmRNA?brd4mRNA?mycmRNA?P53mRNA were detected by real-time fluorescent quantitative PCR(RT-PCR).
Results
different concentrations of JQ1 can all inhibit SUP - B15 cell proliferation, inducing cell apoptosis?apoptosis rate was significantly increased compared to control group, and depend of time and dose. half inhibitory concentration of 72h is about 1.0 umol/L.At the same time?JQ1 can down regulation BCR-ABLmRNA,brd4 mRNA, myc mRNA transcription levels, and up regulation the transcription level of p53mRNA .
Summary
JQ1 as brd4 inhibitor can down regulation the expression of brd4, thus affecting its downstream gene myc and p53, at the same time it can changeover the express of BCR-ABL,further achieve inhibition of cell proliferation, inducing cell apoptosis.
Keyword(s): B cell acute lymphoblastic leukemia, C-myc, Leukemia cell line, P53
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