DACRYOCYTES ARE A COMMON MORPHOLOGIC FEATURE OF AUTOIMMUNE AND MICROANGIOPATHIC HEMOLYTIC ANEMIA
Author(s): ,
Christoph Robier
Affiliations:
Central Laboratory,Hospital of the Brothers of St. John of God,Graz,Austria
,
Daniela Klescher
Affiliations:
Central Laboratory,Hospital of the Brothers of St. John of God,Graz,Austria
,
Gerhard Reicht
Affiliations:
Department of Internal Medicine,Hospital of the Brothers of St. John of God,Graz,Austria
,
Omid Amouzadeh-Ghadikolai
Affiliations:
Department of Psychiatry,Hospital of the Brothers of St. John of God,Graz,Austria
,
Franz Quehenberger
Affiliations:
Medical University of Graz,Graz,Austria
Manfred Neubauer
Affiliations:
Department of Internal Medicine,Hospital of the Brothers of St. John of God,Graz,Austria
(Abstract release date: 05/21/15) EHA Library. Robier C. 06/12/15; 102572; PB1950 Disclosure(s): Hospital of the Brothers of St. John of God, Graz, Austria
Central Laboratory
Christoph Robier
Christoph Robier
Contributions
Abstract
Abstract: PB1950

Type: Publication Only

Background
Dacryocytes are teardrop- or pear-shaped erythrocytes which are usually found in peripheral blood smears of patients with primary or secondary myelofibrosis as well as infiltrative neoplastic disorders of the bone marrow. Such a teardrop poikilocytosis has rarely been observed in blood smears of patients with autoimmune (AIHA) and microangiopathic hemolytic anaemia (MAHA). The  prevalence of dacryocytes in AIHA and MAHA has not been examined to date.

Aims
The aim of this study was to determine the prevalence of dacryocytes in peripheral blood films of patients with AIHA and MAHA.

Methods
We compared the dacryocyte counts in blood smears stained according to the May-Grünwald-Giemsa technique between 20 subjects with AIHA (n=9) and MAHA (n=11) with those from 21 controls having normal peripheral blood counts and lacking signs of hemolysis. The dacryocytes, defined as erythrocytes tapered to a point at one end, were counted as cells per 20 high power fields (HPF) at 630-fold magnification.

Results
In AIHA, MAHA and controls, dacryocytes were found in 89% (8/9), 91% (10/11) and 19% (4/21) of the blood smears, respectively. The medians and ranges of the dacryocyte counts per 20 HPF were 24 (0-89), 11 (0-50) and 0 (0-1).  The rates of dacryocyte positivity and the dacryocyte counts between the samples of hemolytic anemias and controls differed statistically highly significant (p<0.0001).

Summary
The results of this study indicate that dacryocytes are commonly apparent in blood smears of patients with AIHA and MAHA. Knowledge of this frequent feature may be beneficial in clinical routine diagnosis.

Keyword(s): Anemia, Autoimmune hemolytic anemia (AIHA), Red blood cell, Thrombotic microangiopathy
Abstract: PB1950

Type: Publication Only

Background
Dacryocytes are teardrop- or pear-shaped erythrocytes which are usually found in peripheral blood smears of patients with primary or secondary myelofibrosis as well as infiltrative neoplastic disorders of the bone marrow. Such a teardrop poikilocytosis has rarely been observed in blood smears of patients with autoimmune (AIHA) and microangiopathic hemolytic anaemia (MAHA). The  prevalence of dacryocytes in AIHA and MAHA has not been examined to date.

Aims
The aim of this study was to determine the prevalence of dacryocytes in peripheral blood films of patients with AIHA and MAHA.

Methods
We compared the dacryocyte counts in blood smears stained according to the May-Grünwald-Giemsa technique between 20 subjects with AIHA (n=9) and MAHA (n=11) with those from 21 controls having normal peripheral blood counts and lacking signs of hemolysis. The dacryocytes, defined as erythrocytes tapered to a point at one end, were counted as cells per 20 high power fields (HPF) at 630-fold magnification.

Results
In AIHA, MAHA and controls, dacryocytes were found in 89% (8/9), 91% (10/11) and 19% (4/21) of the blood smears, respectively. The medians and ranges of the dacryocyte counts per 20 HPF were 24 (0-89), 11 (0-50) and 0 (0-1).  The rates of dacryocyte positivity and the dacryocyte counts between the samples of hemolytic anemias and controls differed statistically highly significant (p<0.0001).

Summary
The results of this study indicate that dacryocytes are commonly apparent in blood smears of patients with AIHA and MAHA. Knowledge of this frequent feature may be beneficial in clinical routine diagnosis.

Keyword(s): Anemia, Autoimmune hemolytic anemia (AIHA), Red blood cell, Thrombotic microangiopathy

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